If a QuantiTect Primer Assay has more than one version number, does this mean that earlier assay versions may not work?
In most cases, earlier versions of the QuantiTect Primer Assays do work:
- earlier versions may detect both RNA and genomic DNA sequences, while the latest version may be specific for RNA sequences
- there may be only a difference of one nucleotide between the latest and the previous assay version
- earlier versions may not detect all transcript variants, while the latest version can
Previous assay versions can still be used if they work in your experiments. However, we recommend using the latest version. In very rare cases, original versions of the assay may not perform due to significant updates of transcript sequence information in Entrez or Ensembl, which our QuantiTect Primer Assays are based on.
(To find a QuantiTect Primer Assay for your target gene of interest, please visit our GeneGlobe data base).