How can I use the SureSilencing shRNA Plasmids for stable transfection and knock down in a cell line that is already resistant to both neomycin and puromycin?

SureSilencing shRNA Plasmids with other selectable markers are not available at this time, nor do they have convenient restriction sites to reclone the U1-based shRNA expression cassette into another vector expression system. However, you may use a different vector system with another selectable marker (hygromycin), order our SureSilencing shRNA Plasmids with the GFP marker, and perform a co-transfection with these two plasmids in a 10:1 selection marker-to-GFP plasmid molar ratio.

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