What is the composition of buffer OCL?
The composition of Buffer OCL is:
- 10 mM Tris-Cl pH 7.5
- 140 mM NaCl
- 5 mM KCl 1% Nonidet P-40*
Add before use (optional): 1000 U/ml RNase inhibitor; 1 mM DTT
*Nonidet P-40 is no longer manufactured. It can be replaced with Nonidet P-40 Substitute (Fluka, cat. no.74385) or Igepal® CA-630 (SIGMA, cat. no. I 3021).
Buffer OCL is used for direct mRNA purification from the cytoplasm of cultured cells with the Oligotex Direct mRNA Kits.