What method(s) do you recommend as an alternative way to validate the mutations identified on the somatic mutation PCR arrays?

Sanger sequencing and pyrosequencing can be used to validate the mutations identified on the arrays. However, one needs to bear in mind that the detection sensitivity of Sanger sequencing is around 20%, and the detection sensitivity of pyrosequencing is around 5%, while RT-PCR based arrays can detect 1% or lower mutations. Therefore, mutations occurring below the detection limit of Sanger sequencing and pyrosequencing will not be verified by these two methods.

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