What is the SureSilencing shRNA Plasmids guarantee?
We guarantee that at least two of the four SureSilencing shRNA Plasmids in a set will knock down the expression of the target gene by at least 70 percent by real-time qRT-PCR in target shRNA-transfected cells relative to negative control shRNA-transfected cells upon FACS-based enrichment for GFP expression or selection for neomycin, hygromycin, or puromycin resistance. Please follow the recommendations in this User Manual in order to insure the optimal level of knockdown from these plasmids and the best method for detecting knockdown. If you encountered that every plasmid failed to knock down the gene expression as described, please contact a Technical Support representative to discuss your results and be prepared to provide your results in an Excel file as an email attachment. If a product failure is verified, we will send you another set of plasmids with four pre-designed shRNA constructs for free.