What are the features and benefits of the QIAexpress 6xHis Tag System?
|The interaction of the 6xHis tag with Ni-NTA matrices is conformation independent||One-step purification can be carried out under native or denaturing conditions|
|Mild elution conditions can be used||Binding, washing, and elution are highly reproducible, and have no effect on protein structure. Pure protein products are ready for direct use in downstream applications|
|The 6xHis tag is much smaller than other commonly used tags||6xHis tags can be used in any expression system. The Tag does not interfere with the structure and function of the recombinant protein|
|The 6xHis tag is uncharged at physiological pH||The 6xHis tag does not interfere with secretion|
|The 6xHis tag is poorly immunogenic||The recombinant protein can be used without prior removal of the tag as an antigen to generate antibodies against the protein of interest|
|Using Factor Xa Protease, 6xHis tag can be easily and efficiently removed||The detagged protein can be used for crystallographical or NMR studies where removal of the 6xHis tag may be preferred|
|Some QIAexpress vectors feature a 6xHis-dihydrofolate reductase tag (6xHis-DHFR tag)||Small peptides fused to the 6xHis DHFR tag are stabilized while being expressed. The 6xHis-DHFR tag is not highly immunogenic in mouse and rat, so that peptides fused to the tag can be used directly for immunizations or epitope mapping|