What is the composition of Buffer B1?
Buffer B1 is used in combination with lysozyme or lysostaphin and proteinase K for the efficient lysis of bacteria prior to DNA purification using QIAGEN Genomic-tips. Please note this buffer is not recommended for any purification procedures using QIAGEN’s silica-membrane-based spin columns.
Buffer B1 (Bacterial Lysis Buffer 1) consists of 50 mM Tris•Cl pH 8.0; 50 mM EDTA pH 8.0; 0.5% Tween 20; 0.5% Triton-X100.
How to prepare Buffer B1: Dissolve 18.61 g Na2EDTA•2H2O and 6.06 g Tris base in 800 ml distilled water. Add 50 ml 10% Tween 20 solution and 50 ml 10% Triton X-100 solution. Adjust the pH to 8.0 with HCl. Adjust the volume to 1 liter with distilled water.