Why is there no vacuum-only protocol for the RNeasy Plus 96 Kit?

During the gDNA elimination step of the RNeasy Plus 96 procedure, the flow-through of the gDNA Eliminator 96 plate has to be collected. If the plate were to be processed in a vacuum manifold, there would be a substantial risk of cross-contamination due to foaming of the lysate. Also, plate drying and RNA elution are more efficient with a centrifuge than with a vacuum manifold.



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