What are the advantages of REPLI-g over conventional DNA sample processing and amplification methods?
One of the greatest strengths of REPLI-g whole genome amplification (WGA) is extremely low amplification bias at very high levels of amplification. DOP and PEP suffer from allele drop out and high levels of loci bias at greater than 1000-fold amplification. REPLI-g WGA is based on multiple displacement amplification (MDA) and can maintain accurate loci and allele representation even at 1 million-fold amplification with quality gDNA. This advantage comes from the intrinsic high processivity and fidelity of the Phi29 DNA polymerase.
MDA is a method that enhances simple, rapid, isothermal whole-genome amplification. It is carried out directly on crude lysed cells, whole blood, buccal swabs, and other biological samples, eliminating costly, time-consuming DNA purification steps.
REPLI-g WGA significantly increases the amount of DNA from specific samples, allowing to conduct a large number of downstream genetic tests. Conventional methods of sample processing limit the number of tests that can be done, as the amount of DNA available for testing is often small.