Can I use QIAGEN's pQE vectors as templates in the EasyXpress Biotin Labeling Kits?
Yes, QIAGEN's pQE vectors for recombinant 6xHis-tag protein expression have been used successfully with the EasyXpress Site-Specific Biotin Kit and the EasyXpress Random Biotin Kit. They contain the phage T5 promoter, recognized by E. coli polymerase. Alternatively, any T7 promoter based construct could be used. Such vectors provide mRNA-stabilizing secondary structures in the 5' and 3' untranslated regions that play an important role in increasing the efficiency of expression.