I do not see any miRNA expression for my miRNAs of interest after performing single cell miRNA expression analysis with the miScript Single Cell qPCR Kit and miScript miRNA PCR Arrays or miScript Primer Assays. What could be the cause?
To rule out experimental setup errors, first review all handbook protocols. Following this, ensure that real-time PCR quality control has been performed on a representative number of samples/cells, as controls are specifically designed for each reaction associated with the miScript Single Cell qPCR Kit workflow. Once proper performance of the workflow has been confirmed, the possible causes could be that no cell was added during cell lysis, or the assessed miRNAs are not expressed in the particular cell.