Do the SureSilencing shRNA Plasmids contain inducible promoters?

No, SureSilencing shRNA Plasmids with inducible promoters are not available. These promoters are meant to control the timing of silencing particularly for stable shRNA transfections for essential genes or for differentiation model systems. However, they tend to be too leaky for RNA interference. That is, they still express a certain amount of the shRNA of interest even under repressed conditions. We do not recommend a specific vector system for this application. However, you are welcome to screen our shRNA sequences in another cell model system, and then use the shRNA insert sequence information provided with the purchase of our plasmids to have the necessary oligonucleotides synthesize for cloning into your inducible expression system of choice.

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