Can DNA isolated with MaXtract tubes be used for downstream applications such as restriction digestion, labeling, blotting, PCR, and automated sequencing?
DNA purified by organic extraction can principally be used for these downstream reactions. It should be noted that phenol, like all organic solvents, alters the active centre of enzymes, thus deactivating them. An ethanol precipitation should therefore generally be carried out prior to enzyme controlled downstream reactions. Potential phenol residue is thereby effectively removed.
The use of MaXtract Low and High Density tubes has the advantage that no recontamination of the sample by organic solvents or proteins occurs.