How does the built-in QC control for complete bisulfite conversion of DNA work on PyroMark Q24?
Data generated with the methylation analysis software (detection and quantification of CpG sites) on PyroMark Q24 contains unique features that act as quality control for complete bisulfite conversion of DNA. When the assay encounters a C not followed by a G (C unmethylated), that C should be fully converted to T if the bisulfite treatment upfront was successful. Subsequently, it should be presented in the pyrogram as T = 100%. This acts as a useful quality control for full conversion of unmethylated C residues during bisulfite treatment and PCR.