When running RNA samples on the QIAxcel System or QIAxcel Advanced, the standard RNA denaturation process has to be followed prior to running the sample.
- Add an equal volume of QX RNA Denaturation buffer to your RNA sample and/or QX RNA size Marker.
- Heat the solution at 70°C for 2 minutes on a heating block or in a PCR machine, then place on ice for 1 minute.
- Bring the total sample volume to 10 μl using QX RNA Dilution Buffer and mix the solution by gently pipetting up and down a few times.
- Analyze the samples immediately.