When running RNA samples using the QIAxcel System or QIAxcel Advanced, are there any special things to consider?

When running RNA samples on the QIAxcel System or QIAxcel Advanced, the standard RNA denaturation process has to be followed prior to running the sample.

  • Add an equal volume of QX RNA Denaturation buffer to your RNA sample and/or QX RNA size Marker.
  • Heat the solution at 70°C for 2 minutes on a heating block or in a PCR machine, then place on ice for 1 minute.
  • Bring the total sample volume to 10 μl using QX RNA Dilution Buffer and mix the solution by gently pipetting up and down a few times.
  • Analyze the samples immediately.

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