Can I optimize a dPCR assay on the QIAcuity using gradient temperature?

QIAcuity does not support temperature gradient in PCR cycling profile. However, optimization of a dPCR assay can be done by qPCR on a gradient cycler using the dPCR master mix and then transferred from qPCR to dPCR. 

Can’t find what you are looking for?

Browse the FAQ base with our FAQ search.