How much DNA starting sample is needed for analysis with a Somatic Mutations PCR Array? And Assay?

The recommended starting DNA sample for the Somatic Mutation PCR Arrays is 500 ng for a 96-well plate, and 200 ng for a 384-well plate. If using less, please proceed with Whole Genome Amplification (see User Manual for protocol); if using DNA isolated from FFPE samples, 500 ng – 2 ug is recommended for 96-well plates & 200 ng – 1.6 ug is recommended for 384-well plates. For Somatic Mutation Assays, it is recommend to start with 5 – 40 ng.

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