3273 - At what temperature can I perform the experiment with the NexTal Cubic Phase kits?
Temperature is the most important factor in the experiments with the NexTal Cubic Phase kits. In fact, in meso phase crystallization is very temperature sensitive. The working range is between 20 and 24°C, but, 22°C is the optimal temperature. Below these temperatures, MO crystal-like structures will form and will be very difficult to distinguish from protein crystals. Also, the chance of obtaining protein crystals will be very low. Lower temperatures can be used if the lipidic phase contains, for example, monoolein + 8% cholesterol. In this case, temperatures as low as 18°C are used. Note: All GPCR crystals reported in the literature so far were crystallized between 20°C to 24°C.