What is the minimum amount of genomic DNA required for analysis using EpiTect Methyl qPCR Arrays?

For digestion, we recommend using 0.5-4ug (0.125-1ug per digestion) of genomic DNA for EpiTect Methyl qPCR Arrays and 0.25-0.5ug (0.0625-0.125ug per digestion) for single qPCR assays. However, you should not use less than 0.25ug (0.0625ug per digestion) of genomic DNA for digestion. For PCR reactions, we recommend using 5-10ng per PCR reaction and at least 2ng genomic DNA should be included in each PCR reaction.

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