What denaturation temperature should be used in cycling conditions?

VeraSeq 2.0 DNA Polymerase is highly thermostable and it is recommended that denaturation should be performed at 98°C. The initial denaturation time can be extended from 30 seconds up to 3 minutes for difficult templates. For subsequent denaturation cycles, 5–10 seconds at 98°C is sufficient for most targets. 

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