How can I optimize Mg2+ conditions for a specific amplicon when using Phoenix Hot Start Taq and the supplied reaction buffer?

Supplied Phoenix Hot Start Taq buffers contain 2 mM Mg2+ in the final reaction. Therefore, final Mg2+ reaction concentration may be increased according to user preference with a concentrated solution containing Mg2+.

Note: Increasing final Mg2+ concentration may lower fidelity of the PCR (1).     

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