Can I use the PhosphoProtein Purification Kit to purify phosphorylated peptides of around 1 kD size?

We have not tested the PhosphoProtein Purification Kit with phosphorylated peptides, but we would predict that it should work fine. However, note that the Nanosep Ultrafiltration Columns for concentrating protein fractions will not be suitable due to their cutoff size of 10 kD. The peptides have to be solubilized in, or dialysed against the PhosphoProtein Lysis Buffer before applying the sample to the PhoshoProtein Purification Column.


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