Why are there various different QuantiTect Primer Assays for my gene (previous versions, transcript variants)?
QuantiTect Primer Assays are designed using sequences from the Entrez and Ensembl databases, which are both updated regularly. New sequence information may allow us to design improved assays that detect all transcript variants and/or avoid detection of genomic DNA.
Some assays may have been originally designed using sequences only from Entrez because the sequences were not available in Ensembl or because there were discrepancies between Entrez and Ensembl. Subsequent updates to Ensembl may have allowed us to design an improved assay.
For certain genes, we also offer QuantiTect Primer Assays that detect specific transcript variants. These assays can be used in experiments in which only a specific splice variant is to be detected.
To find a QuantiTect Primer Assay for your target gene of interest, please visit our GeneGlobe data base.