Why is there no QuantiTect Primer Assay for my gene of interest?

Our QuantiTect Primer Assays for the genomes of human, rat, mouse, and many other species cover >90% of the transcripts listed in the NCBI Reference Sequence (RefSeq) database (e.g., for human, >98% of the transcripts are covered). The RefSeq database is a comprehensive, integrated, nonredundant and curated collection of sequences, including genomic DNA, transcript RNA, and protein products for major research organisms.

Lack of certain QuantiTect Primer Assays for specific transcripts can be due to unreliable or missing sequence information, or due to the fact that no assay can be found which meets our high-stringency assay design criteria.

Regular updates to our QuantiTect Primer Assay database are performed every 8–12 weeks, depending on the organism, so a QuantiTect Primer Assay for your gene of interest may be available via GeneGlobe in the future.


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