QIAsymphony SP for FFPE sample preparation

For automated, high throughput DNA prep from FFPE tissue samples

Products

In the context of the GeneReader NGS System, the QIAsymphony SP, QIAsymphony DPS DNA Mini Kit and QIAsymphony GeneRead FFPE Treatment Kits are intended for Research Use Only. Not for use in diagnostic procedures.
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QIAsymphony GeneRead DNA FFPE Treatment Kit  (192)

Cat. No. / ID: 185306

For uracil-N-glycosylase treatment of FFPE tissue specimens using the QIAsymphony SP Tissue_LC_200_V7_DSP protocol
$462.00
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QIAsymphony SP

Cat. No. / ID: 9001297

QIAsymphony sample prep module: includes 1-year warranty on parts and labor
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QIAsymphony DSP DNA Mini Kit (192)

Cat. No. / ID: 937236

For 192 preps of 200 µl each: Includes 2 reagent cartridges and enzyme racks and accessories.
$1,110.00
The QIAsymphony DSP DNA Mini Kit (192) is intended for in vitro diagnostic use.

Features

  • Rapid purification of high-quality, ready-to-use DNA for use in research applications
  • Enzymatic removal of cytosine deamination artifacts for FFPE samples
  • Automation on the QIAsymphony Sample Preparation
  • Outstanding results in DNA sequencing applications

Product Details

With the QIAsymphony DSP DNA Mini Kit, in combination with the QIAsymphony GeneRead DNA FFPE Treatment Kit and QIAsymphony SP, it is possible to fully automate DNA extraction from up to 96 FFPE samples in parallel. Enzymatic removal of cytosine deamination artifacts from FFPE samples in combination with optimized protocols provide high yields of pure DNA, suitable for downstream sequencing applications in the GeneReader NGS System workflow. Novel, prefilled reagent cartridges reduce setup time and minimize manual handling. Bar code reading enables full tracking of reagents.

Principle

The QIAsymphony DSP DNA mini kit in combination with the QIAsymphony GeneRead DNA FFPE Treatment Kit can be used to purify DNA from FFPE tissues. This combines the speed and efficiency of silica-based purification of DNA with the convenient handling of magnetic particles (see figure " Schematic of the QIAsymphony SP principle"). Innovative, ready-to-run reagent cartridges are prefilled with all reagents required for the purification procedure and are automatically opened by the QIAsymphony SP, which helps to minimize the risk of environmental contamination (see figure " Prefilled, sealed reagent cartridges"). Worktable setup is rapid, which saves you valuable time. Simply place up to 2 reagent cartridges in the consumables drawer. The reagent cartridges can be either from the same kit or from different kits, enabling different purification procedures to be performed within the same run of 96 samples. Reagent volumes only for the selected number of samples are used, giving you complete cost control.
See figures

Procedure

The purification procedure is designed to ensure safe and reproducible handling of potentially infectious samples, and comprises 4 steps: lyse, bind, wash, and elute (see figure " QIAsymphony DSP DNA procedure"). Optimized protocols provide high quality nucleic acids with efficient removal of proteins, nucleases, and other impurities. Nucleic acids purified with QIAsymphony GeneRead DNA FFPE Treatment Kit and QIAsymphony DSP DNA Mini Kit is ready for direct use in downstream library preparation and sequencing applications on the GeneReader NGS System. The user can choose between different elution volumes.
See figures

Applications

Designed for use in the GeneReader NGS System workflow.

Supporting data and figures

Service Plans

QIAsymphony SP, Preventive Subscription

Cat. No. / ID: 9243545A

One on-site Preventive Maintenance or Inspection Service visit for the QIAsymphony SP, including travel, labor and parts. Includes a 10% discount on repair services during the Preventive Subscription period.

Resources

Application/Protocol Documents (27)
For use with the QIAsymphony AS (software version 4.0 or higher)
For use with the QIAsymphony AS (software version 4.0 or higher)
For use with the QIAsymphony AS (software version 4.0 or higher)
For use with the QIAsymphony AS (software version 4.0 or higher)
For use with the QIAsymphony AS (software version 4.0 or higher)
For use with the QIAsymphony AS (software version 4.0 or higher)
For use with the QIAsymphony AS (software version 4.0 or higher)
For use with the QIAsymphony AS (software version 4.0 or higher)
For use with the QIAsymphony AS (software version 4.0 or higher)
For use with the QIAsymphony AS (software version 4.0 or higher)
For use with the QIAsymphony AS (software version 4.0 or higher)
For use with the QIAsymphony AS (software version 4.0 or higher)
For use with the QIAsymphony AS (software version 4.0 or higher)
For use with the QIAsymphony AS (software version 4.0 or higher)
For use with the QIAsymphony AS (software version 4.0 or higher)
For use with the QIAsymphony AS (software version 4.0 or higher)
For use with the QIAsymphony AS (software version 4.0 or higher)
For use with the QIAsymphony AS (software version 4.0 or higher)
For use with the QIAsymphony AS (software version 4.0 or higher)
For use with the QIAsymphony AS (software version 4.0 or higher)
For use with the QIAsymphony AS (software version 4.0 or higher)
For use with the QIAsymphony AS (software version 4.0 or higher)
For use with the QIAsymphony AS (software version 4.0 or higher)
For use with the QIAsymphony AS (software version 4.0 or higher)
For use with the QIAsymphony AS (software version 4.0 or higher)
For use with the QIAsymphony AS (software version 4.0 or higher)
For use with the QIAsymphony AS (software version 4.0 or higher)
Supporting Software and Tools (2)
For QIAsymphony software version 5.0.4
Work List Tool
SOFTWARE (1MB)
For QIAsymphony Software version 5.0
Protocol Files (8)
For use with the QIAsymphony SP/AS instruments (software version 4.0 or higher)
For use with the QIAsymphony SP/AS instruments (software version 4.0 or higher)
For use with the QIAsymphony SP/AS instruments (software version 4.0 or higher)
For use with the QIAsymphony SP/AS instruments (software version 5.0 or higher)
For use with the QIAsymphony SP/AS instruments (software version 4.0 or higher)
For use with the QIAsymphony SP/AS instruments (software version 4.0 or higher)
For use with the QIAsymphony SP instruments (software version 4.0 or higher)
Instrument User Manuals (5)
For use with QIAsymphony SP/AS instruments (software version 5.0)
For use with QIAsymphony SP/AS instruments (software version 5.0)
For use with QIAsymphony SP/AS instruments (software version 5.0)
For use with QIAsymphony SP/AS instruments (software version 5.0)
For use with QIAsymphony SP/AS instruments (software version 5.0)
Application/Protocol Documents for IVD Use (11)
DNA_Blood_200_V7_DSP protocol

QIAsymphony DSP DNA Kit Protocol Sheet_V2_In Vitro Diagnostic use according to the Regulation (EU) 2017/746 on in vitro diagnostics medical devices

DNA_Buffy_Coat_200_V7 DSP protocol

QIAsymphony DSP DNA Kit Protocol Sheet_V2_In Vitro Diagnostic use according to the Regulation (EU) 2017/746 on in vitro diagnostics medical devices

VirusBlood200_V5_DSP protocol

QIAsymphony DSP DNA Kit Protocol Sheet_V2_In Vitro Diagnostic use according to the Regulation (EU) 2017/746 on in vitro diagnostics medical devices

Tissue_LC_200_V7_DSP and Tissue_HC_200_V7_DSP protocols

QIAsymphony DSP DNA Kit Protocol Sheet_V2_In Vitro Diagnostic use according to the Regulation (EU) 2017/746 on in vitro diagnostics medical devices

For use with software version 4.0 or higher
For use with software version 4.0 or higher
For use with software version 4.0 or higher
For use with software version 4.0 or higher
For use with software version 4.0 or higher
For use with software version 4.0 or higher
For use with software version 4.0 or higher
Brochures & Guides (6)
Instrument Technical Documents (10)
QIAsymphony SP recovery procedures for DNA Investigator applications for software version 4.0 and HID 1.0
QIAsymphony SP recovery procedures for DNA Investigator applications for software version 4.0 and HID 1.0
QIAsymphony SP recovery procedures for DNA Investigator applications for software version 4.0 and HID 1.0
QIAsymphony SP recovery procedures for DNA Investigator applications for software version 4.0 and HID 1.0
QIAsymphony Cabinet SP/AS documents
QIAsymphony Cabinet SP/AS document
Software document for QIAsymphony software version 4.0.1
For use with QIAsymphony SP/AS instruments (software version 5.0)
Differences between QIAsymphony software versions
Labware Files and Documents (2)
Labware package SOW-516-0-08 for use with the QIAsymphony SP/AS instruments (software version 5.0)
Labware Documents (1)
For use with software version 5.0 or higher
Kit Handbooks (2)
QIAsymphony DSP DNA Kit Handbook_V2_In Vitro Diagnostic use according to the Regulation (EU) 2017/746 on in vitro diagnostics medical devices

FAQ

What RNA elution volumes are available with the QIAsymphony RNA Kit?

RNA processed on the QIAsymphony SP with the QIAsymphony RNA Kit can be eluted in 200, 100, or 50 µl.

Elution in smaller volumes will provide higher concentration, but lower overall yield. Total RNA yield with elution in 50 µl will be about 20% lower compared to elution in 100 µl.

 

FAQ ID -1943
What are the starting volumes of homogenate for RNA extraction on the QIAsymphony SP?

Using the Standard protocol for RNA extraction on the QIAsymphony SP, up to 400 µl of lysate can be processed while the Large-Volume protocol allows up to 800 µl of homogenate.

 

 

 

 

FAQ ID -1939
Can DNA yields be increased by prolonging incubation with proteinase K in Buffer ATL?

For most sample types, incubating longer than specified in the pretreatment protocol for the QIAsymphony DNA Investigator Kit does not increase yields. However, lysates can be incubated overnight without negatively affecting the quality of the extracted DNA.

 

FAQ ID -2032
Is there a charge for user training on the QIAsymphony SP System?

No. Sample handling, software, and introduction to maintenance for the QIAsymphony SP System are included in the installation training and are covered by the installation fee.

 

 

FAQ ID -1900
Are there any specific cleaning solutions recommended for the QIAsymphony SP?

The following disinfectants and detergents are recommended for cleaning the QIAsymphony SP:

  • Mikrozid® Tissues (Schülke & Mayr GmbH) — 150 tissues with 235 g agent (consists of 24g (94%) ethanol and 35g 1-propanol per 100g agent)
  • Mikrozid Liquid (Schülke & Mayr GmbH) — ethanol-based disinfectant for spraying onto surfaces (consists of 25 g ethanol and 35 g 1-propanol per 100 g agent solution)
  • RBS®-35 Detergent Concentrate (Pierce Chemical Co.) — anionic and nonionic surfactant based detergent for cleaning the touch screen and worktable door.
  • RNaseZap® (Ambion) — for complete removal of RNase contamination from glass and plastic surfaces
  • 0.1 M NaOH — for cleaning the worktable and racks to remove RNase contamination (alternative to RNaseZap)

Europe

  • Lysetol® AF (Gigsept® Instru AF) (Schülke & Mayr GmbH) — disinfectant for submerging worktable items (consists of 14 g cocospropylene-diamine-guanidine diacetate, 35 g phenoxypropanols, and 2.5 g benzalkonium chloride per 100 g Lysetol AF, with anticorrosion components, fragrance, and 15-30% nonionic surfactants

USA

  • DECON-QUAT® 100 (Veltek Associates, Inc.) — quaternary ammonium salt based disinfectant concentrate for submerging worktable items (contains 5% alkyldimethylbenzylammonium chloride and 5% alkyldimethylethylbenzylammonium chloride)

Note: If you want to use disinfectants different from those recommended, ensure that their compositions are similar to those described above.

FAQ ID -1913
Can I program my own protocols for the QIAsymphony SP?

No. Modified QIAGEN protocols or custom protocols for QIAsymphony SP can be developed to meet your specific requirements and purchased from QIAGEN.

 

 

 

FAQ ID -1904
Does an internal control have to be included for each QIAsymphony Virus/Bacteria protocol run?

No. The use of internal controls with the QIAsymphony Virus/Bacteria Kits is not mandatory. When using internal controls, they are added to the carrier RNA mixture (total volume of the mixture must be 120 µl).

 

FAQ ID -2040
Can uneven sample numbers be processed on the QIAsymphony SP module?

Yes. Any number of samples between 1 and 96 can be processed on the QIAsymphony SP.

 

 

FAQ ID -1906
Can the QIAsymphony RNA Kit be used to purify RNA from blood?

For purification of RNA from blood on the QIAsymphony SP, we recommend using the QIAsymphony PAXgene RNA Kit, since it includes stabilization of gene expression profiles.

 

 

FAQ ID -1945
Why do I have to use an ACS (Assay Control Set)?
The ACS specifies the combination of a protocol and a predefined carrier RNA–internal control mixture. The user can define an internal control mixture using the QIAsymphony Management Console. A predefined internal control-carrier RNA-Buffer AVE mixture can be automatically identified by the instrument using a bar code label or it can be chosen manually.
FAQ ID -2933
Which QIAsymphony Virus/Bacteria Kit size should be used for each protocol?

The QIAsymphony Virus/Bacteria Mini Kit is used with the 'Cellfree_200' and 'Complex_200' protocols.

The QIAsymphony Virus/Bacteria Midi Kit is used with the 'Cellfree_500’, 'Cellfree_1000', ‘Complex_400’ and 'Complex_800' protocols.

FAQ ID -2038
What is the part number for O-rings for QIAsymphony SP and AS instruments? How often should the O-rings be replaced?

Part number for O-rings for pipetting channels of QIAsymphony SP and AS instruments is 9019168. There are 32 O-rings included in one bag.

You will also require the tool to change the O-rings. Part number for this tool is: 9019164. This tool set includes one bag of 32 O-rings.

We recommend replacing O-rings on all pipetting channels, once a month.

              

FAQ ID -3055
What sample types can be processed using the tissue protocols and the QIAsymphony DSP DNA Mini Kit?
Tissue and FFPE tissue can be processed using the tissue protocols and the QIAsymphony DSP DNA Mini Kit on the QIAsymphony SP.
FAQ ID -2925
Can solid samples be processed with the QIAsymphony DNA Investigator Kit on the instrument?

No. All solid materials (e.g., swabs, blood card punches, chewing gum) must be removed completely from the lysate before performing automated extraction on the QIAsymphony SP. QIAshredder columns can be used to increase sample recovery from some solid materials. For more information, see the corresponding pretreatment protocol.

 

FAQ ID -2027
Is sample pretreatment required for purifying RNA on the QIAsymphony SP?

For purification of RNA on the QIAsymphony SP, tissue samples require mechanical disruption and homogenization in the supplied lysis buffer (Buffer RLT Plus), e.g., by using the TissueLyser or TissueRuptor. Cultured cells should be lysed by vortexing in the lysis buffer contained in the kit.

 

FAQ ID -1938
How can I create a report/logfile on my QIAsymphony SP system?

Please navigate to Tools/Instrument Report/Create + Save to USB.

FAQ ID - 3594
Does carrier RNA have to be added using the QIAsymphony Virus Blood 200 Protocol?

No. We do not recommend use of carrier RNA with the QIAsymphony Virus Blood 200 Protocol.

 

FAQ ID -2163
How does magnetic-particle carry-over in QIAsymphony eluates need to be handled?

In general, magnetic particles are not carried over into QIAsymphony eluates. However, if high amounts of genomic DNA are purified (large number of white blood cells in the sample) magnetic-particle carryover may be observed. If carryover does occur, magnetic particles in eluates will not affect most downstream applications. If magnetic particles need to be removed before performing downstream applications, tubes or plates containing eluates should first be placed in a suitable magnet and the eluates transferred to a clean tube.

 

FAQ ID -2166
How much starting material can be used with the QIAsymphony DSP DNA Mini Kit on the QIAsymphony SP?

Tissue: If no information about the expected yield is available, we recommend starting with 25 mg sample material. Depending on the yield obtained, the sample size can be increased in subsequent preparations to a maximum of 50 mg.

FFPE: Up to 4 sections, each with a thickness of up to 10 µm, or 8 sections with a thickness of up to 5 µm and a surface area of up to 250 mm2, can be combined in one preparation.

FAQ ID -2927
How should DNA isolated on the QIAsymphony be stored?

Eluates obtained on the QIAsymphony SP can be stored at 2–8°C for 3 days before use in downstream applications and should be placed at –20°C or –80°C for long-term storage.

 

 

FAQ ID -2165
What sample types can be processed using the QIAsymphony Virus/Bacteria protocols?

Plasma, serum, and CSF samples can be processed using the Virus Cellfree protocols of the QIAsymphony Virus/Bacteria Kits. Respiratory samples (sputum, BAL, aspirates, dried swabs, transport media), and urogenital samples (urine, urogenital swabs, transport media) can be processed using the Pathogen Complex protocols.

 

FAQ ID -2036
Do tissue samples require pretreatment before loading onto the QIAsymphony SP for DNA extraction?
Yes. Samples have to be completely lysed and homogenized prior to automated processing on the QIAsymphony SP platform using the QIAsymphony DSP DNA Mini Kit. Tissue samples are lysed by proteinase K digestion. FFPE tissue samples are deparaffinized with Deparaffinization Solution or xylene/ethanol and lysed by proteinase K digestion. Insoluble material is pelleted by centrifugation. Lysates are placed on the QIAsymphony SP for purification of DNA (see protocol sheets for details).
FAQ ID -2926
What are the expected DNA yields from tissue processed on the QIAsymphony SP?

DNA yields strongly depend on the type and source of the tissue processed on the QIAsymphony SP. Approximately 70–80 µg DNA can be isolated from 25 mg of spleen, 40–50 µg from 25 mg liver, and 10–15 µg from 50 mg muscle.

 

FAQ ID -1919
What sample input volumes can be used on the QIAsymphony SP?
200–1000 µl sample volumes can be processed on the QIAsymphony Sample Prep Module.
FAQ ID -1907
How much starting material can be used with the QIAsymphony DNA Mini Kit on the QIAsymphony SP?

The maximum amount of tissue that can be processed on the QIAsymphony SP using QIAsymphony DNA Mini Kit is 50 mg, and the maximum number of cultured cells is 1 x 107.

See details for upper limits of various starting materials in the table below:

 

Sample type Sample amount Protocol
Heart 25 mg Tissue Standard
Spleen 25 mg Tissue High Content
Lung 25 mg Tissue Standard
Liver 25 mg Tissue High Content
Kidney 25 mg Tissue Standard
Muscle 50 mg Tissue Standard
Mouse tail 0.8 cm Tissue Standard
Jurkat Cells 2 x 10e6 Tissue Standard
Jurkat Cells 1 x 10e7 Tissue High Content

FAQ ID -1917
How many samples can be processed with the QIAsymphony DNA Mini Kit and the Virus Blood 200 protocol?

A total of 192 samples can be processed with one QIAsymphony DNA Mini Kit and the Virus Blood 200 protocol (96 samples per reagent cartridge).

 

 

 

FAQ ID -2159
Can protocol runs on the QIAsymphony SP be interrupted and resumed?

QIAGEN protocols on the QIAsymphony SP can be stopped if there is an emergency by pressing "Pause". To confirm that you want to stop the protocol run, press "Stop". To cancel the stop protocol command, press "Continue".

Note: If a protocol run is stopped, the run cannot be restarted. Samples must be processed manually. For more information, refer to the QIAsymphony User Manual.

 

FAQ ID -1912
Is the QIAsymphony RNA Kit compatible with stabilized samples?

Yes, the QIAsymphony RNA Kit is compatible with stabilized samples. We recommend stabilizing tissue samples with RNAprotect Tissue Reagent or AllProtect Tissue Reagent, or by flash-freezing. Cultured cells can be lysed directly in cell culture dishes, or after pelleting. Alternatively, cells can be stabilized using RNAprotect Cell Reagent.


FAQ ID -1944
What sample types can be processed using the QIAsymphony DSP Virus/Pathogen protocols?
Plasma, serum, and CSF samples can be processed using the QIAsymphony DSP Virus/Pathogen cellfree protocols. Respiratory samples (sputum, BAL, aspirates, dried swabs, transport media), and urogenital samples (urine, urogenital swabs, transport media) can be processed using the pathogen complex protocols.
FAQ ID -2928
What sample volumes can be used with the Virus Blood 200 Protocol on the QIAsymphony?

Sample volumes of 200 µl can be processed with the Virus Blood 200 Protocol using the QIAsymphony DNA Mini Kit.

 

 

FAQ ID -2156
What sample volumes can be used with the tissue HC and LC protocols on the QIAsymphony SP?
After pretreatment, 220 µl of the lysates must be transferred to the QIAsymphony SP to ensure that 200 µl of sample is processed.
FAQ ID -2992
What are the recommended lysis times for tissue samples on the QIAsymphony SP?
Lysis time varies depending on the tissue type processed. For recommended lysis times refer to the tissue protocol sheets. If lysis is incomplete after recommended lysis time, as indicated by the presence of insoluble material or highly viscous lysates, lysis time can be prolonged or insoluble material can be removed by centrifugation. Overnight lysis is possible and does not affect the preparation.
FAQ ID -2993
Do samples require pretreatment before loading onto the QIAsymphony SP for DNA extraction?

Yes. Samples have to be completely lysed and homogenized prior to automated processing on the QIAsymphony SP platform using QIAsymphony DNA Kits.

Samples are lysed by Proteinase K digestion in lysis buffers optimized for tissue, cultured cells, and bacteria, respectively. For lysis of gram-positive bacteria, lysozyme is used in addition to Proteinase K. Lysed samples are homogenized by pipetting up and down, and insoluble material is removed by centrifugation (see the QIAsymphony DNA Handbook for details).

 

FAQ ID -1916
How should samples be stored before purification using the Virus Blood 200 Protocol on the QIAsymphony?

Samples should be processed on the QIAsymphony within 24 hours of collection. Samples should be transported and stored at 2–25°C.

 

 

 

FAQ ID -2157
Should sample lysate volumes be exactly 200 µl, 500 µl, or 1000 µl when loaded on the QIAsymphony SP?

No. The specified lysate volumes are optimal for QIAsymphony DNA Investigator Kit protocols. Lower volumes (150 µl, 400 µl, 900 µl, respectively) can be used.

However, to maximize yields, we recommend transferring as much of the lysate as possible. QIAshredder columns can be used to increase recovery from some sample types. For more information, see the corresponding pretreatment protocol.

 

FAQ ID -2031
What are the dimensions and weight of the QIAsymphony SP System?

The QIAsymphony SP instrument has the following weight and dimensions:

  • Weight: 178 kg (392.4 lb.); 220 kg (485 lb.) with packaging
  • Width: 130 cm (51.2 in.)
  • Depth: 75 cm (29.5 in.); 125 cm (49.2 in.) with drawer open
  • Height (door closed): 103 cm (40.6 in.)
  • Height (door open): 163 cm (64.2 in.)
FAQ ID -1901
Can the QIAsymphony SP Module be connected to a laboratory information management system (LIMS)?

Yes, QIAsymphony SP can be connected to a lab information management system. Result files can be exported in html format. At a later date, users will be able to import sample worklists to the QIAsymphony SP.

 

FAQ ID -1909
Which types and amounts of sample material can be processed for RNA extraction on the QIAsymphony SP?

The QIAsymphony RNA Kit enables purification of RNA from cultured cells and easy-to-lyse tissues, such as kidney, liver, and spleen on the QIAsymphony SP. A large-volume protocol is available that enables processing of larger sample amounts.

  • Cultured cells: up to 3x106 cells (standard protocol); up to 1x107 (large volume protocol)
  • Tissues: up to 20 mg of most tissues (standard protocol); up to 50 mg (large-volume protocol)

For particularly DNA-rich tissues, such as spleen or thymus, we recommend either using no more than half the maximum amount, or using DNase at a higher concentration (see the QIAsymphony RNA Handbook; additional DNase required).

FAQ ID -1937
What sample volumes can be processed with the QIAsymphony Virus/Bacteria Kits?

Sample volumes that can be processed with the QIAsymphony Virus/Bacteria Kits depend on the protocol:

  • 'Cellfree_200' and 'Complex_200':  200 µl
  • 'Complex_800':                            800 µl
  • 'Cellfree_1000':                           1000 µl

 

FAQ ID -2037
Which sample types can be processed using the QIAsymphony Virus Blood 200 protocol?

Human whole blood samples treated with EDTA or citrate can be processed using the QIAsymphony Virus Blood 200 protocol with the QIAsymphony DNA Mini Kit. We do not recommend using heparin-treated blood samples.

 

 

 

FAQ ID -2154
Will mitochondrial DNA also be isolated using the DNA Tissue protocols on the QIAsymphony SP?

Yes. The DNA Tissue protocols on the QIAsymphony SP purify genomic and mitochondrial DNA.

 

 

 

 

FAQ ID -1921
How can I determine if a tube is suitable for clot detection on QIAsymphony?

You can find this information in the respective labware list. All tubes which require an insert in the sample input carrier or plates are not suitable.

FAQ ID - 3590
Is mitochondrial DNA isolated with the QIAsymphony DNA Investigator Kit on the QIAsymphony SP?

Yes. QIAsymphony DNA Investigator Kit protocols purify both genomic and mitochondrial DNA.

 

 

 

FAQ ID -2030
How long can homogenates remain on the QIAsymphony SP without risk of RNA degradation?

Strongly denaturing conditions in the lysis buffer (Buffer RLT Plus) of the QIAsymphony RNA Kit keep RNases in an inactive state. Keeping homogenates on the QIAsymphony instrument at room temperature (20-25°C) for over 6 hours does not impair RNA integrity.

 

FAQ ID -1941
Which elution volumes can be selected using the QIAsymphony Virus Blood 200 protocol?

Final elution volumes of 60 µl, 85 µl, 110 µl, or 165 µl can be selected using the QIAsymphony Virus Blood 200 protocol. Please note that the initial elution volumes are 95 µl, 120 µl, 145 µl, or 200 µl. When calculating the amount of internal control(s) as well as the titer of the processed sample, it is necessary to take into consideration the initial volume of elution buffer that is used for each sample.

 

FAQ ID -2160
Is it possible to modify QIAsymphony protocols?

No. Protocols on the QIAsymphony SP cannot be modified by users. However, protocols allow elution volumes to be selected within a predefined range. Customized protocols for QIAsymphony SP will be available on request.

 

FAQ ID -1903
Which barcodes on sample tubes are accepted by the QIAsymphony SP?

Code 39, code 128 and subtypes, Codabar

FAQ ID - 3598
What sample types should be processed with the High Content (HC) protocol for DNA extraction on the QIAsymphony SP?

The High Content protocol for DNA extractions on the QIAsymphony SP is optimized for samples with an expected DNA yield of more than 30 µg. Up to 90 µg DNA can be purified from tissues with high cell densities (e.g., spleen) or large numbers of cultured cells.

 

FAQ ID -1918
Is clot detection possible on the QIAsymphony?
Yes, clot detection is possible on the QIAsymphony.
FAQ - ID 3588
How does QIAsymphony SP keep cellular samples in suspension prior to pipetting so that samples do not settle?
Samples are mixed before pipetting by the QIAsymphony SP Module.
FAQ ID -1905
Does the QIAsymphony SP system generate run reports and/or log files?

Yes. At the end of each protocol run on the QIAsymphony SP, a report file is generated that contains important information such as number of samples, liquid volumes measured during the load check, and run time. A log file is also generated that contains information about instrument actions, such as movement of the robotic arm.

 

 

FAQ ID -1911
What is the range of elution volumes on the QIAsymphony SP?

Depending on the application, elution volumes on the QIAsymphony SP range from 30 µl to 500 µl.

 

 

 

FAQ ID -1908
What kind of samples should be processed using the large-volume protocol with the QIAsymphony DNA Investigator Kit?

The large-volume protocol of the QIAsymphony DNA Investigator Kit is intended for samples that can not be submerged completely during lysis in volumes less than 1 ml, as well as samples that are very absorbent.

 

FAQ ID -2034
What are the expected yields of total RNA isolated with the QIAsymphony SP?

Please see expected yields of total RNA isolated with the QIAsymphony RNA Kit on the QIAsymphony SP in the table below:

 

Sample type Sample amount Typical RNA yield (µg)*

Cultured Cells

   
HeLa, Jurkat 1 x 10e6 cells 15

Rat tissues

   
Kidney 10 mg 10-25
Liver 10 mg 20-50
Lung 10 mg 5-10
Spleen 10 mg 25-35

*RNA yields strongly depend on the type and quality of the sample material.

FAQ ID -1942
What sample types can be processed using the tissue protocols for DNA extraction on the QIAsymphony SP?

Various types of tissue, cultured cells, and bacterial pellets can be processed using the tissue protocols for the QIAsymphony DNA Kits on the QIAsymphony SP.

 

 

 

 

FAQ ID -1915
Do QIAsymphony Virus/Bacteria samples require pretreatment before they are loaded onto the instrument?

Pre-treatment of samples used with the QIAsymphony Virus/Bacteria Kits can improve nucleic acid extraction for some sample types. Viscous samples (e.g., sputum, respiratory aspirates) might require liquefaction to enable pipetting. Pretreatment protocols are provided in the QIAsymphony Virus/Bacteria Handbook.

 

FAQ ID -2039
Is RNA co-purified with DNA using QIAsymphony DNA Tissue protocols?

Yes. Magnetic particles used in the QIAsymphony SP System will bind RNA and total DNA. If isolation of RNA-free DNA is required, RNase A must be added to the lysate before isolation of DNA.

 

FAQ ID -1920
What is the thermal output for a QIAsymphony SP and AS instrument?

Power consumption of a QIAsymphony SP instrument is 800 VA. Therefore, the approximate thermal output is 2729 BTU/h.

If the QIAsymphony SP is connected to a QIAsymphony AS, then the power consumption is 1400 VA. The thermal output is ~4777 BTU/h.

 

FAQ ID -3057
What is the maximum DNA yield from forensic samples processed with the QIAsymphony SP?

QIAsymphony DNA Investigator Kit protocols are optimized for maximum sensitivity in extraction of typical forensic-casework and reference sample types, which usually have low DNA content. Due to optimized bead capacity, maximum DNA yields are approximately 3 µg.

 

FAQ ID -2028
In which tubes is clot detection possible on QIAsymphony?
Only tubes with a diameter < 15 mm can be used for clot detection on the QIAsymphony.

FAQ ID - 3589

What sample types should be processed with the low content (LC) and high content (HC) protocols for DNA extraction on the QIAsymphony SP?
Tissues will give increased DNA yields when processed with the high content protocol, but the low content protocol in combination with a small elution volume (50µl) may be used if high DNA concentration is required. For FFPE tissue we recommend using the low content protocol.
FAQ ID -2989
Do all QIAsymphony DNA Investigator protocols use carrier RNA?

No. Carrier RNA is only used in casework protocols for the QIAsymphony DNA Investigator Kit to enable efficient extraction of minute quantities of DNA. Carrier RNA is not used in reference protocols.

 

FAQ ID -2033
Does an internal control have to be used with the QIAsymphony Virus Blood 200 Protocol?

No. The use of internal controls is not mandatory for use with the QIAsymphony Virus Blood 200 Protocol. If an internal control is not required, Buffer ATE without internal control is added to the samples. Buffer ATE must be placed in slot A of the sample drawer.

 

FAQ ID -2162
What are the expected DNA yields from tissue processed on the QIAsymphony SP?
DNA yields strongly depend on the type and source of the tissue processed on the QIAsymphony SP. For FFPE tissue samples, the DNA yield depends highly on the fixation and processing of the tissue material. QIAsymphony magnetic particles copurify RNA and DNA if both are present in the sample. RNA may influence photometric analysis and may lead to overestimated nucleic acid yields. If RNA-free DNA is required, add RNase A in the step indicated in the respective pretreatment protocol (see protocol sheets for details).
FAQ ID -2990
How can the QIAsymphony SP system be decontaminated?
To decontaminate the QIAsymphony SP, refer to the QIAsymphony User Manual.
FAQ ID -1914
Why do casework-sample protocols for the QIAsymphony DNA Investigator Kit start with different lysate volumes (200 µl, 500 µl, or 1000 µl)?

To ensure performance with the QIAsymphony DNA Investigator Kit, lysis volumes have been optimized according to sample type. In general, large or absorbent samples require larger buffer volumes than small and non-absorbent samples.

 

FAQ ID -2035
What is elution Buffer ATE of the QIAsymphony DNA Investigator Kit?

Buffer ATE of the QIAsymphony DNA Investigator Kit is a low-EDTA elution buffer optimized for long-term storage of DNA as well as performance in sensitive downstream applications. Alternatively, casework protocols using water for elution are available.

 

Buffer ATE contains a small amount of the preservative sodium azide. If the eluate is measured for absorbance the spectrophotometer should be blanked with the buffer ATE as sodium azide absorbs strongly at 230 nm. 

 

FAQ ID -2029
How many samples can be processed per kit or per reagent cartridge for RNA purification on the QIAsymphony SP?

Each QIAsymphony RNA Kit enables preparation of up to 192 samples (96 per reagent cartridge) using the standard protocol on the QIAsymphony SP. If only the large-volume protocol is used, the number of samples that can be prepared per kit is reduced to 96 (48 per reagent cartridge). Any combination of standard and large-volume sample preparation can be performed.

 

FAQ ID -1940
Will mitochondrial DNA also be isolated using the DNA tissue protocols and QIAsymphony DSP DNA Mini Kit on the QIAsymphony SP?
Yes. The DNA tissue protocols on the QIAsymphony SP purify genomic and mitochondrial DNA.
FAQ ID -2991
What is the target nucleic acid for the Virus Blood 200 Protocol on the QIAsymphony?

The target nucleic acid for the Virus Blood 200 Protocol on the QIAsymphony is viral DNA from released as well as cell-associated viruses, co-purified with genomic DNA from blood cells.

 

 

FAQ ID -2158