Freshly harvested tissue is submerged in Allprotect Tissue Reagent to immediately preserve the in vivo profile of DNA, RNA, and protein. Tissue samples in Allprotect Tissue Reagent are stabilized for long-term storage without freezing and can be stored at room temperature for up to 7 days or at 2–8°C for up to 12 months.
The reagent is part of QIAGEN’s range of sample technologies for systems biology, and can be used in combination with the AllPrep DNA/RNA/Protein Mini Kit, which allows simultaneous purification of DNA, RNA, and protein from the same precious sample. Allprotect stabilized tissues are also compatible with QIAGEN kits for purification of DNA, RNA, or protein, such as DNeasy, QIAamp, RNeasy, and Qproteome Kits. Functional protein for enzyme assays can be purified from Allprotect stabilized tissues using the Qproteome Mammalian Protein Prep Kit. There is no need to split the sample into 3 prior to purification, allowing maximum recovery of DNA, RNA, and protein. In addition, no organic solvents such as phenol and acetone are required. DNA, RNA, and protein are all prepared from the same source, eliminating the variation inherent in preparing these analytes from different samples.
QIAGEN sample technologies for individual purification of DNA, RNA, and protein are also available (see table). These include the Qproteome Mammalian Protein Prep Kit, which allows purification of functional protein from Allprotect stabilized tissues. Protein purified from Allprotect stabilized tissue with the Qproteome Mammalian Protein Prep Kit is suitable for all downstream applications.
QIAGEN kits compatible with Allprotect Tissue Reagent
|Genomic DNA, total RNA, and total protein
||AllPrep DNA/RNA/Protein Mini Kit
|Genomic DNA and total RNA
||AllPrep DNA/RNA Micro Kit
AllPrep DNA/RNA Mini Kit
AllPrep DNA/RNA/miRNA Universal Kit
||DNeasy Blood & Tissue Kit
QIAamp DNA Mini Kit
||RNeasy Plus Micro Kit* (for small tissue samples)
RNeasy Mini Kit (for easy-to-lyse tissues)
RNeasy Plus Mini Kit* (for easy-to-lyse tissues)
RNeasy Fibrous Tissue Mini Kit (for fibrous tissues)
RNeasy Lipid Tissue Mini Kit (for fatty tissues)
RNeasy Microarray Tissue Mini Kit
(for tissue samples for microarray analysis)
||Qproteome Mammalian Protein Prep Kit