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HiSpeed Plasmid Kits

For ultrafast purification of up to 750 µg transfection-grade plasmid or cosmid DNA
  • Less than 60 minutes prep time
  • Lysate clearing and isopropanol precipitation without centrifugation
  • No risk of DNA pellet loss during precipitation
  • Up to 750 µg yield of high-copy plasmid DNA
  • LyseBlue for optimum lysis and maximum DNA yield
  • Large-scale plasmid preparation without vacuum manifold

HiSpeed Plasmid Kits provide fast, large-scale, anion-exchange-based plasmid DNA preparation without centrifugation. The purified DNA is equivalent to that obtained by 2 x CsCl gradient centrifugation and is suitable for transfection-grade applications.

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Cat No./ID: 12643
HiSpeed Plasmid Midi Kit (25)
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25 HiSpeed Midi Tips, 25 QIAfilter Midi Cartridges, 25 QIAprecipitator Midi Modules plus Syringes, Reagents, Buffers
Cat No./ID: 12662
HiSpeed Plasmid Maxi Kit (10)
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10 HiSpeed Maxi Tips, 10 QIAfilter Maxi Cartridges, 10 QIAprecipitator Maxi Modules plus Syringes, Reagents, Buffers
Cat No./ID: 12663
HiSpeed Plasmid Maxi Kit (25)
Order Product
25 HiSpeed Maxi Tips, 25 QIAfilter Maxi Cartridges, 25 QIAprecipitator Maxi Modules plus Syringes, Reagents, Buffers
HiSpeed Plasmid Kits are intended for molecular biology applications. These products are not intended for the diagnosis, prevention, or treatment of a disease.


Product Details


HiSpeed Plasmid Kits contain QIAfilter Cartridges, HiSpeed Tips, and QIAprecipitator modules for fast large-scale plasmid preparation without vacuum manifold. Syringe-format QIAfilter and QIAprecipitator modules replace centrifugation steps in the classic anion-exchange procedure, making plasmid purification faster and more convenient. Up to 750 µg (maxi) or 200 µg (midi) high-copy plasmid DNA can be purified from 150 µl – 250 ml or 50 µl – 150 ml culture, respectively (culture volumes depend on plasmid copy number, size of insert, host strain, and culture medium). HiSpeed Tip design allows a very high flow rate, permitting DNA binding, washing, and elution steps for plasmid purification to proceed faster.

The unique anion-exchange resin in HiSpeed Tips is developed exclusively for the purification of nucleic acids. Its exceptional separation properties result in DNA purity equivalent or superior to that obtained by two successive rounds of CsCl gradient centrifugation.


QIAfilter Cartridges (see figure "QIAfilter Cartridge") are special filter units designed to replace the centrifugation step following alkaline lysis of bacterial cells. QIAfilter Cartridges completely remove SDS precipitates and clear bacterial lysates in a fraction of the time needed for centrifugation. Prepacked HiSpeed Tips operate by gravity flow and never run dry, minimizing the hands-on time required for plasmid preparation.

The unique QIAprecipitator module (see figure "QIAprecipitator module") replaces the centrifugation step traditionally used to collect isopropanol-precipitated DNA following purification. The QIAprecipitator module traps the precipitated DNA, while the isopropanol-buffer mixture flows through. The DNA is then simply eluted from the QIAprecipitator into a microcentrifuge tube with TE buffer or water. This unique module also eliminates the risk of pellet loss, which can occur during decanting of the supernatant following centrifugation.

Features HiSpeed Plasmid Maxi Kit HiSpeed Plasmid Midi Kit
Applications Transfection, cloning sequencing, gene silencing Transfection, cloning sequencing, gene silencing
Culture volume/starting material 150 µl – 250 ml culture volume 50 µl – 150 ml culture volume
Plasmid type High-copy, cosmid DNA High-copy, cosmid DNA
Processing Manual (filtration) Manual (filtration)
Sample per run 1 sample per run 1 sample per run 
Technology Anion-exchange technology Anion-exchange technology
Time per run 60 min 45 min 
Yield <750 µg <200 µg


Neutralized bacterial lysates are incubated in the QIAfilter Cartridge and cleared in seconds by filtration. The filtrate is applied to a HiSpeed tip for plasmid DNA purification (see flowchart "QIAGEN Plasmid Kit procedures"). Eluted DNA is mixed with isopropanol and applied to the QIAprecipitator module using the syringe provided. The concentrated and desalted DNA is then eluted from the QIAprecipitator directly into a microcentrifuge tube with TE buffer or water. 


DNA purified with HiSpeed Plasmid Kits yield excellent results in all applications, from cloning and sequencing to transfection and plasmid-mediated gene silencing.

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