DNeasy PowerSoil HTP 96 Kit

For the isolation of DNA from up to 384 soil samples in less than one day

Features

  • Fast, high-throughput format processes up to 250 mg of sample in 96 wells
  • Easy isolation of DNA from tough microbes using the PowerLyzer 24 Homogenizer
  • Completely eliminates PCR inhibitors with Inhibitor Removal Technology
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DNeasy PowerSoil HTP 96 Kit (384)

Cat. No. / ID: 12955-4

For the isolation of DNA from up to 384 soil samples in less than one day
$2,302.00
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DNeasy PowerSoil HTP 96 Kit
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The DNeasy PowerSoil HTP 96 Kit is intended for molecular biology applications. This product is not intended for the diagnosis, prevention, or treatment of a disease.

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Product Details

Processing large amounts of soil and other environmental samples is fast and easy with the DNeasy PowerSoil HTP 96 Kit. Using a 96-well format, difficult soil types from compost to marine sediment to manure can be successfully processed and the DNA isolated. The highly pure DNA resulting from processing with the DNeasy PowerSoil HTP 96 Kit is ready for downstream applications, including qPCR amplification.

Samples are broken down and homogenized on the 96-well bead beating plate. Cells are lysed by both mechanical and chemical means and the DNA is captured on a 96-well silica membrane spin column, washed and eluted. The DNA is then ready for downstream applications such as qPCR or next-generation sequencing.

PCR analysis on resulting DNA has detected a variety of organisms, from yeasts, molds and other fungi, algae, Gram (+/-) bacteria (including Bacillus subtilis, Bacillus anthracis), actinomycetes (including Streptomyces) and nematodes.

DNeasy PowerSoil HTP 96 Kit was formerly sold by MO BIO as PowerSoil-htp 96 Well Soil DNA Kit.

Specifications

FeaturesSpecifications
FormatSilica Spin Filter Plates
ProcessingBead beating
Binding capacityUp to 20 µg per prep
Sample size0.25 g
Throughput96-384 samples
Time per run or per prepProcess 384 samples in less than one day
Storage temperatureStore at room temperature(15-30°C)
Bead size0.7 mm garnet
Sample typesProcessed soil, fecal, water, food, insects, swabs with PCR inhibitors

Resources

Quick-Start Protocols (1)
Safety Data Sheets (2)
Download Safety Data Sheets for QIAGEN product components.
Download Safety Data Sheets for QIAGEN product components.

FAQ

Are there important considerations for plasma generation and urine handling?

It is strongly advised to follow the recommendations for preparing sample material provided in the corresponding Protocol Sheet to ensure reliable results.

Plasma: It is recommended to perform plasma separation immediately after blood collection when using EDTA or citrate as anticoagulant to prevent the release of genomic DNA into the plasma fraction.

Urine: Because circulating cell-free DNA in non-stabilized urine samples is rapidly degraded after sample collection due to high nuclease activity, eluates may contain no DNA or exhibit low DNA concentration. Therefore, it is recommended to stabilize urine samples. Even when using stabilized urine, it is recommended to perform a centrifugation step immediately after stabilization to prevent the release of genomic DNA from cells. Alternatively, non-stabilized urine samples can be processed immediately after collection and centrifugation using ATL-pretreatment and automated DNA extraction as described in the corresponding Protocol Sheet.

FAQ ID - 3699