Rotor-Gene Q MDx (US)

For in vitro diagnostic use with FDA cleared or approved nucleic acid tests in clinical laboratories


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Rotor-Gene Q MDx Platform (US)

Cat. No. / ID:  9002035

Real-time PCR cycler with 6 channels (the red and HRM channels are not intended for use with FDA cleared or approved nucleic acid tests), laptop computer, software, accessories, 1-year warranty on parts and labor
Service Options
The Rotor-Gene Q MDx instrument is intended for in vitro diagnostic use with FDA-cleared or -approved nucleic acid tests in clinical laboratories.

✓ 24/7 automatic processing of online orders

✓ Knowledgeable and professional Product & Technical Support

✓ Fast and reliable (re)-ordering


  • Optimal thermal and optical performance due to rotary format
  • Robust technology, requiring minimal maintenance
  • Easy-to-use software with advanced security features

Product Details

The Rotor-Gene Q MDx instrument, with Rotor-Gene Q software version 2.1.0 or higher, is a real-time nucleic acid amplification and detection system, which measures nucleic acid signals from amplified DNA using fluorescent detection.


Unique rotary design for outstanding performance

The Rotor-Gene Q MDx uses a sophisticated heating and cooling design to achieve optimal reaction conditions. The unique rotary format ensures optimal thermal and optical uniformity between samples, which is critical for precise and reliable analysis (see figure " Cross-section of the Rotor-Gene Q MDx"). Each tube spins in a chamber of moving air, keeping all samples at precisely the same temperature during rapid thermal cycling. Detection is similarly uniform. When each tube aligns with the detection optics, the sample is illuminated and the fluorescent signal is rapidly collected from a single, short optical pathway. This thermal and optical uniformity results in sensitive, precise, and fast real-time PCR analysis.

Robust optics for multiplex applications

With a choice of up to 6 excitation sources and 6 detection filters (the red and HRM channels are not intended for use with FDA cleared or approved nucleic acid tests), combined with a short, fixed optical path, the Rotor-Gene Q MDx can be used for multiplex reactions, ensuring minimum fluorescence variability between samples and eliminating the need for calibration or compensation.

Samples are excited from the bottom of the chamber by a light-emitting diode (see figure " Cross-section of the Rotor-Gene Q MDx"). Energy is transmitted through the thin walls at the base of the tube. Emitted fluorescence passes through emission filters on the side of the chamber and is then collected by a photomultiplier. The fixed optical path ensures consistent excitation for every sample, which means that there is no need to use a passive internal reference dye such as ROX.

Channels for optical detection
Channel Excitation (nm)Detection (nm)
Green 470 ± 10 510 ± 5
Yellow 530 ± 5 557 ± 5
Orange 585 ± 5  610 ± 5
Red* 625 ± 5 660 ± 10
Crimson 680 ± 5 712 high pass
HRM* 460 ± 20 510 ± 5
Minimum maintenance; maximum convenience

The Rotor-Gene Q MDx is engineered to reduce the need for maintenance, and to maximize ease of use. This saves time and costs, and keeps the instrument operational for continuous testing.

Convenient features of the Rotor-Gene Q MDx include:

  • Lifetime guarantee on highly stable LEDs, no expensive and time consuming lamp replacement, and no gradual performance loss
  • Cost-effective and easy temperature-accuracy verification
  • Optical calibration not required at installation, or when the instrument is moved
  • "Plug and play" connectivity between the computer and the instrument
  • No sample block to clean
  • No condensation or bubbles in the reaction due to rotation
  • Small, light, and robust instrument — simply place it where you want it
See figures


Nucleic acids are purified using the designated manual or automated purification kit that is validated for the downstream assay. After assay setup, the assays are then transferred to the Rotor-Gene Q MDx for PCR and data analysis.

Easy routine verification

Optical temperature verification (OTV) is a method that verifies the in-tube temperature in a Rotor-Gene Q MDx. While it is not required for the Rotor-Gene Q MDx, calibration of in-tube temperature can be a laboratory requirement. The OTV method provides a means for users to comply with this requirement, including if there are site-specific calibration intervals. OTV is performed using a Rotor-Disc OTV Kit. The full procedure takes less than 30 minutes and can be performed by any user.


The Rotor-Gene Q MDx instrument is intended for in vitro diagnostic use with FDA cleared or approved nucleic acid tests in clinical laboratories.


Software enables quantification and enhances data security

The user-friendly software provides simplicity for beginners as well as an open experimental platform for advanced users. Assay packages contain the required files to run and analyze individual types of assays.

Data security, from starting the run to exporting the results, is assured by audit trails, digital signatures, and individual user management. Each Rotor-Gene Q MDx instrument is supplied with an unlimited software user license for your institute, enabling you to access and analyze your test results on as many computers as you want — in the laboratory, or in the office.

Supporting data and figures


Heat dissipation/thermal loadAverage, 0.183 kW (632 BTU/hour); Peak, 0.458 kW (1578 BTU/hour)
Operating temperature18-30°C (64-86°F)
Humidity10-75% (noncondensing)
Power100-240 V AC, 50-60 Hz, <520 VA (peak); Power consumption <60 VA (standby); Mains supply voltage fluctuations are not to exceed 10% of the nominal supply voltages; F5a 250 V fuse
Place of operationFor indoor use only
Pollution level2
Optical System6 channels; Excitation sources: High energy light-emitting diodes; Detector: Photomultiplier; Acquisition time: 4 seconds
Overvoltage categoryII
Samples per run (throughput)Strip Tubes 0.1 ml (4 tubes) - 72 samples/run; Tubes 0.2 ml (not intended for use with FDA cleared or approved nucleic acid tests) - 36 samples/run; Rotor-Disc 72 (not intended for use with FDA cleared or approved nucleic acid tests) - 72 samples/run; Rotor-Disc 100 (not intended for use with FDA cleared or approved nucleic acid tests) - 100 samples/run
DimensionsWidth, 37 cm (14.6 in.); height, 28.6 cm (11.3 in.); Depth (without cables), 42 cm (16.5 in.); Depth (door open), 53.8 cm (21.2 in.)
SoftwareRotor-Gene Q software 2.1.0 or higher, supplied on the installation CD provided
Storage conditions15°C to 30°C (59°F to 86°F) in manufacturer's package; Max. 75% relative humidity (noncondensing)
TechnologyReal-time PCR cycler
Thermal performanceTemperature range, 35°C to 99°C (95°F to 210.2°F); Temperature accuracy, ±0.5°C; Temperature resolution, ±0.02°C (smallest programmable increment); Temperature uniformity, ±0.02°C (standard deviation)
Transportation conditions-25°C to 60°C (-13°F to 140°F) in manufacturer's package; Max. 75% relative humidity (noncondensing)
Warranty1 year on instrument; lifetime guarantee on excitation LEDs
Weight12.5 kg (27.6 lb.), standard configuration
AltitudeUp to 2000 m (6500 ft)


Instrument User Manuals (2)
For use with Rotor-Gene Q Software version 2.3.4
US Version; for use with Rotor-Gene Q Software version 2.3.5 or higher
Operating Software (1)
For use on the Rotor-Gene Q. Rotor-Gene Q software 2.3.5 is compatible with Windows 7 and Windows 10 operating systems
Safety Data Sheets (1)
Certificates of Analysis (1)


Please refer to section 'Guidelines for effective multiplex assays' under "Important Notes" in the Rotor-Gene Multiplex PCR Handbook for suitable combinations of reporter dyes, or visit our Multiplex real-time PCR Resource site for additional information.
FAQ ID -9028
What reaction volume is suitable for use in the Rotor-Gene Q?

Reaction volumes suitable for use on the Rotor-Gene Q are:

  • Rotor-Disc 100: 30 µl x 100-wells, 10-25 µl reaction volume
  • Rotor-Disc 72: 0.1 ml x 72-wells, 15-25 µl reaction volume
  • Strip Tubes 0.1 ml: 0.1 ml x 72-wells, 10-30 µl reaction volume, strips of 4 tubes and caps
  • PCR Tubes 0.2 ml: 0.2 ml x 36-wells, 15-50 µl reaction volume, individual tubes with caps
FAQ ID -9030
Why is no fluorescence signal detected in my run?

Please make data are collected in the appropriate fluorescent channel. Also check the gain is optimized.

If the issue persists, please send the original run file with extension .rex to QIAGEN Technical Service for further assistance.

FAQ ID -9023
What should I do if the Rotor-Disc OTV run does not pass?

Please send the original OTV run file to QIAGEN Technical Service for further assistance.

FAQ ID -9022
What kind of file is required for hardware-related trouble-shooting?
For hardware related issues, please send the support package to QIAGEN Technical Service. Within the Rotor-gene Q software, click Help and select Send Support Email. In the new window, select the file that relates to the issue and email it to QIAGEN Technical Service.
FAQ ID - 9024
Is regular calibration needed with the Rotor-gene instrument?
QIAGEN recommends the annual inspection service on Rotor-gene instruments, during which all application-critical modules of the Rotor-gene are inspected and tested and an OTV check is conducted. Performed tests and test results are documented in a GMP/GLP-compliant Report. In addition, the end users can perform the temperature calibration in the lab as needed using the Rotor-Disc OTV kit.

Note: The Rotor-Disc OTV kit requires the Rotor-Disc 72 Rotor and Rotor-Disc 72 locking ring.
FAQ ID -9025
Can I import a standard curve from a previous PCR run on the Rotor-Gene Q?

Use of endogenous control genes corrects for variation in RNA content, variation in reverse-transcription efficiency, possible RNA degradation or presence of inhibitors in the RNA sample, variation in nucleic acid recover, and differences in sample handling. The endogenous control gene ought to have consistent expression levels between samples and among treatment conditions, and ideally has a similar expression level to that of the genes of interest. Genes commonly used as references can be found at the QuantiTect Primer Assays as endogenous controls.

FAQ ID -9027
Must I fill blank positions with empty tubes when running sample numbers lower than the rotor capacity of the Rotor Gene Q?
Yes, all empty positions in the rotor of the Rotor-Gene Q have to be filled with empty tubes. This guarantees optimal temperature repartition in the Rotor-Gene Q chamber.
FAQ ID -9029