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References

References

  1. Freshney, R.I. (1993) Culture of Animal Cells, A Manual of Basic Technique, 3rd ed., New York: Wiley-Liss.
  2. Spector, D., Goldman, R.R., and Leinwand, L.A., eds. (1998) Cells: a Laboratory Manual. Cold Spring Harbor, NY: Cold Spring Harbor Laboratory Press.
  3. Drexler, H.G. et al., eds. (1997) DSMZ Catalog of Human and Animal Cell Lines. 6th ed.

Ausubel, F.M. et al. eds. (1991) Current protocols in molecular biology. New York: Wiley Interscience.

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Previous Freezing and viability staining of cells
    • Animal cell cultures
      • Primary cell cultures
      • Finite cell cultures
      • Continuous cell lines
    • Safety and handling considerations for animal cell culture
      • Legislation and regulatory guidelines
      • Safety considerations and biohazards
      • Handling cell cultures
      • Aseptic technique and minimization of aerosols
      • Laminar flow hoods
    • Contamination
      • Microbial contamination
      • Mycoplasmal infection — detection
      • Mycoplasmal infection — eradication
      • Cross-contamination of cell lines
    • Cell culture conditions
      • Media and serum
      • Incubation conditions
      • Cell culture vessel
      • Cell banking
      • Culture instability
    • Essential protocols for animal cell culture
      • Maintaining cell cultures
      • Cell thawing
      • Trypsinizing cells
      • Passaging cells
    • Counting cells
      • Cell counting using a hemocytometer
    • Freezing and viability staining of cells
      • Freezing of cells
      • Viability staining
    • References