Next-Generation Sequencing

NGS solutions for genomic surveillance of SARS-CoV-2
High-throughput NGS solutions for reliable detection of emerging SARS-CoV-2 variants

The emergence of new SARS-CoV-2 variants such as B.1.1.7 and B.1.351 is changing the trajectory of the pandemic. Cataloging these variants and evaluating the impact of mutations is critical for effective vaccine development and the implementation of a robust public health response. Partner with QIAGEN to expedite SARS-CoV-2 variant detection and phylogeographic tracking using reliable, high-throughput NGS technologies and integrated bioinformatics pipelines. From genomic surveillance to metatranscriptomics – uncover the multiple facets of SARS-CoV-2 biology with precision.

New benchmark study evaluates SARS-CoV-2 whole genome sequencing protocols for variant detection
A recent study by Liu et al. revealed that the QIAseq SARS-CoV-2 Primer Panel enabled preferential amplification of the SARS-CoV-2 genome by up to 100-fold in human samples, compared to human or bacterial genomes. In comparison with RNA-seq metagenomics-based technologies, it also achieved >100-fold higher coverage of the SARS-CoV-2 genome, depending on viral load and sequencing depth.
SARS-CoV-2 genomic surveillance

With SARS-CoV-2 rapidly mutating, there is an increased need for high-throughput whole viral genome sequencing to accelerate genomic surveillance for deeper phylogenetic and phylogeographic insights. Detect new SARS-CoV-2 variants with precision using high-throughput QIAseq NGS technology. Based on optimized publicly-available ARTIC V3 designs, the QIAseq SARS-CoV-2 Primer Panel provides targeted enrichment of the complete viral genome. By minimizing primer dropouts and primer-dimer effects, the panel ensures high coverage even at low copy number. Combine with the QIAseq FX DNA Library UDI Kit to multiplex up to 384 samples in a single run on high-throughput Illumina platforms. Track and visualize phylogenetic divergence with our integrated bioinformatics analysis pipelines. Expedite variant detection and real-time genomic surveillance with our scalable, single-day workflow, which is faster and has lower-input requirements than hybrid capture solutions.

SARS-CoV-2 metatranscriptomics

Deciphering the complex interplay between the host and its microbiota can unlock detailed insights into host, microbiome, virus and pathway responses. Highly abundant host and bacterial rRNA can be problematic in coronavirus metatranscriptomics studies, leading to wasted RNA-seq reads. To maximize unique gene expression reads, remove >95% of both host and bacterial rRNA in just 1 hour, while still retaining viral RNAs by using QIAseq FastSelect –rRNA HMR Kits and QIAseq FastSelect –5S/16S/23S Kits either separately or in combination. Then move on to stranded, high-quality library prep using the QIAseq Stranded Total RNA Lib Kit for sensitive detection of low-expression RNA molecules with increased complexity and transcript coverage. Benefit from the specificity and efficiency of the complete QIAseq workflow to expedite SARS-CoV-2 metatranscriptomics research.

Evaluate QIAseq SARS-CoV-2 NGS technologies in your lab – request a trial kit
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Analyze SARS-CoV-2 sequence data with ease

Make sense of your SARS-CoV-2 sequencing data with integrated bioinformatics. Intuitive and easy-to-use QIAGEN CLC Genomics Workbench enables visualization and comparison of multiple reads and variants simultaneously. From phylogenetic to metagenomic analyses, accelerate impactful insights with our integrated bioinformatics solutions for SARS-CoV-2 genomic surveillance.

Coronavirus research publications using QIAGEN technologies
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SARS-CoV-2 Whole Genome Sequencing Services

Ramp up SARS-CoV-2 genomic surveillance efforts by taking advantage of our comprehensive SARS-CoV-2 Whole Genome Sequencing Services. Extend your in-house resources with the expertise and custom services that you expect from QIAGEN.

All photos taken prior to COVID-19
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