Unravel host-microbiome interactions to advance coronavirus metatranscriptomics research
Deciphering the complex interplay between the host and its microbiota can unlock detailed insights into host, microbiome, virus and pathway responses. If you’re working with mixed human and bacterial samples from nasopharyngeal, lung and other tissues for SARS-CoV-2 metatranscriptomics research, highly abundant host and bacterial rRNA can be problematic, leading to wasted reads.
Improve RNA-seq sensitivity in SARS-CoV-2 metatranscriptomics
The more effective your rRNA removal strategy, the greater your ability to achieve usable reads in RNA-seq. To maximize unique gene expression reads, remove >95% of both host and bacterial rRNA in just 1 hour, while still retaining viral RNAs by using QIAseq FastSelect –rRNA HMR Kits and QIAseq FastSelect –5S/16S/23S Kits either separately or in combination. Then move on to stranded, high-quality library prep using the QIAseq Stranded Total RNA Lib Kit for sensitive detection of low-expression RNA molecules with increased complexity and transcript coverage.
Benefit from the specificity and efficiency of the complete QIAseq workflow to expedite SARS-CoV-2 metatranscriptomics research.
SARS-CoV-2 metatranscriptomics solutions
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QIAseq FastSelect –rRNA/Globin KitFor rapid rRNA and/or globin mRNA removal for RNA-seq library preparation from human, mouse, rat and other mammalian samples
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QIAseq FastSelect –5S/16S/23S KitFor rapid 5S/16S/23S rRNA removal for RNA-seq library preparation from bacterial RNA samples
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QIAseq Stranded Total RNA Lib KitFor preparation of stranded whole transcriptome RNA-seq libraries for NGS applications on Illumina instruments
Get started with a trial kit
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QIAseq FastSelect –rRNA/Globin Kit
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QIAseq FastSelect –5S/16S/23S Kit
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QIAseq Stranded Total RNA Lib Kit
Publications
Webinars
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rRNA removal for RNA-seq applications involving viruses, host responses and metatranscriptomicsSpeaker: Samuel Rulli, Ph.D., Associate Director, RNA-seq profiling, NGS assay technologies, QIAGEN -
Targeted RNA panels to identify SARS-CoV-2 and study host gene expressionSpeaker: Samuel Rulli, Ph.D., Associate Director, RNA-seq profiling, NGS assay technologies, QIAGEN