dsDNA Mass Moles Converter

This tool converts the mass of dsDNA to moles of dsDNA and moles of dsDNA to the mass of dsDNA.

Mass to Moles
Moles to Mass
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Mass to Moles Converter

moles of dsDNA [mol] = dsDNA mass [g] / DNA molecular weight [g/mol]
moles of dsDNA ends [mol] = moles of dsDNA [mol] x 2

DNA copy number [molecules] = moles of dsDNA [mol] x 6.022e23 [molecules/mol]
DNA molecular weight [g/mol] = (DNA length [bp] x 617.96 [g/mol per bp]) + 36.04 [g/mol]

Note: 617.96[g/mol] per bp is average molecule weight where sequence not given or if sequence is given sum of A: 617.44 [g/mol], T: 617.44 [g/mol], C: 618.43 [g/mol], G: 618.43 [g/mol]

Moles to Mass Converter

dsDNA mass [g] = DNA moles [mol] xDNA molecular weight [g/mol]
DNA molecular weight [g/mol] = (DNA length [bp] x 617.96 [g/mol per bp]) + 36.04 [g/mol]

Note: 617.96[g/mol] per bp is average molecule weight where sequence not given or if sequence is given sum of A: 617.44 [g/mol]; T: 617.44 [g/mol]; C: 618.43 [g/mol]; G: 618.43 [g/mol]

 

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This converter is highly used in molecular biology applications involving quantifying DNA samples based on their mass and converting them to moles. This conversion is vital for various experimental procedures, such as PCR (polymerase chain reaction), DNA sequencing, cloning and gene expression analysis.

Here are some specific applications where you may use the dsDNA Mass Moles Converter:

1. DNA quantification: Determining the DNA concentration in a sample is crucial for many experiments. The converter helps researchers calculate the amount of DNA in moles based on the mass of dsDNA.
2. PCR and qPCR: Polymerase chain reaction (PCR) is used to amplify specific DNA sequences, while quantitative PCR (qPCR) allows for quantifying DNA or RNA targets. The converter can accurately determine the amount of DNA to be used as a starting template or in calculating standard curves for absolute quantification.
3. DNA sequencing: Prior to DNA sequencing, the concentration and purity of the DNA sample need to be determined. The converter assists in converting the mass of dsDNA into moles, which is necessary for accurate quantification and subsequent library preparation for sequencing.
4. Cloning experiments: The converter helps researchers determine the DNA required for specific cloning reactions in molecular cloning. This ensures the appropriate stoichiometry of DNA fragments, such as vectors and inserts, for successful cloning.
5. Gene expression analysis: Techniques like quantitative reverse transcription PCR (qRT-PCR) and RNA-seq rely on accurate quantification of DNA or RNA. The converter aids in determining the amount of starting material required for such analyses.

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