
These good housekeepers polish, snip and trim
Reagents and reactants that are left behind can hinder downstream processes. Use the right enzyme preparation to clean up your samples before taking the next step. Speed up and enhance amplification and transcription with some not-so-secret additives.
Enzymes for cleavage and cleanup of residual reagents
Enzymatic cleanup of DNA samples with Exonuclease I, DNase I, RNase A, Proteinase K and other nucleases and proteases removes residual primers, nucleotides and enzymes prior to SNP analysis, next-generation sequencing, Sanger DNA sequencing, bioprocessing or other downstream procedures.
Proteinase K is a broad-spectrum endopeptidase widely used for the digestion of proteins, including DNases and RNases. A digestion step with the enzyme is routinely applied during nucleic acid preparation without affecting the integrity of isolated DNA or RNA. Proteinase K is active under a wide range of reaction conditions, including elevated temperatures and the presence of SDS.
Proteinase K NGS Grade is available for the most demanding applications, including preparation of nucleic acids for next-generation sequencing. Extensive purification yields a high-quality enzyme product with increased specific activity, significantly increased solubility (2.5 fold) and remarkable purity with DNA content ≤0.1 pg/mg. Proteinase K NGS Grade is free of exonucleases, endonucleases and ribonucleases.
The TAGZyme DAPase enzyme and TAGZyme system is used for His-tag removal from proteins containing an intrinsic DAPase stop point (expressed using the TAGZyme pQE-2 vector) or from proteins that contain an engineered glutamine stop point.
A comprehensive range of housekeeping enzymes is available for cleavage and cleanup of residual reagents.
Enzyme | Activity | Application |
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RP100B, RP101B, RP103B, RP107B Proteinase K (molecular biology grade) |
BLIRT Proteinase K (MB/PCR grade) is a subtilisin-related serine protease isolated from Parengyodontium album (Tritirachium album) |
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RP100N, RP101N, RP102N, RP103N Proteinase K Ultrapure (NGS grade) |
BLIRT Proteinase K (Ultrapure/NGS grade) is a subtilisin-related serine protease isolated from Parengyodontium album (Tritirachium album); extensive purification yields the highest quality enzyme product |
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19131 Proteinase K |
QIAGEN Proteinase K is a subtilisin-type protease isolated from the saprophytic fungus Tritirachium album |
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19155 Protease |
QIAGEN Protease is a serine protease isolated from a recombinant Bacillus strain; an economical alternative to Proteinase K |
Protease digestion in isolation of native DNA and RNA from a variety of sources |
X8010L Exonuclease I Coming soon |
Exonuclease I cleaves single-stranded DNA in the 3’→5’ |
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79254 RNase-free DNase |
Endonuclease that nonspecifically cleaves DNA to release di-, tri- and oligonucleotide products with 5’-phosphorylated and 3’-hydroxylated ends; acts on ssDNA and dsDNA, chromatin and RNA:DNA hybrids |
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EN31 dsDNase-HL BLIRT |
Heat-labile endonuclease derived from a cold-water eukaryote; high specific activity towards dsDNA leaving ssDNA or RNA undamaged; easily inactivated by moderate heat treatment |
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EN33 dsDNase BLIRT |
Endonuclease derived from marine amphipods; high specific activity towards dsDNA leaving ss DNA or RNA undamaged; highly active in a broad spectrum of temperatures, buffer conditions and pH |
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EN32 Saltonase (HL-Nuclease) BLIRT |
Cold-active, heat-labile endonuclease originating from psychrophilic bacteria; digests all DNA and RNA substrates in different buffer conditions and a broad range of temperatures, including low temperatures and environments with high salt content |
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Endoribonuclease that degrades ssRNA at C and U residues |
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34362 TAGzyme DAPase Enyzme |
TAGZyme DAPase (Recombinant dipeptidyl peptidase I) |
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Improving yields
Improved PCR yields and quality of templates may be achieved with the use of proteins that counteract PCR inhibitors and by adding specialized DNA-binding proteins to amplification and sequencing reactions. The DNA-binding protein, gene 32 protein from bacteriophage T4 (T4gp32), increases PCR amplification efficiency with a number of diverse templates. In addition, using E. coli single-stranded DNA-binding protein (SSB) in DNA sequencing reactions increases the resolution of sequencing runs.
An increased rate of in vitro transcription is made possible by treatment with inorganic pyrophosphatase, an essential component of reactions for RNA preparation. This enzyme cleaves pyrophosphate into two phosphate molecules and prevents pyrophosphate from precipitating with magnesium.
Reagent | Activity | Application |
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Y9030L E. coli ssDNA Binding Protein Coming soon |
Binds with high specificity to single-stranded DNA; thermostable |
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Y9130L T4 Gene 32 Protein Coming soon |
Stabilizes ssDNA regions |
Increases processivity of some DNA polymerases |
RP50, RP51 PCR Anti-Inhibitor |
Mixture of alkaline proteins to counteract substances that inhibit PCR originating in DNA template isolation |
Increases PCR yields with difficult DNA templates isolated from urine, saliva, sputum, blood, cell swabs, cerebrospinal fluid, biopsies, etc. |
Y9380L E. coli pyrophosphatase Coming soon |
An inorganic pyrophosphatase that catalyzes the hydrolysis of
inorganic pyrophosphate to form orthophosphate* |
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Y9370L Thermostable pyrophosphatase Coming soon |
Thermostable pyrophosphatase is a recombinant enzyme from Sulfolobus acidocaldarius that catalyzes the hydrolysis of inorganic pyrophosphate to produce orthophosphate† |
Useful for the enhancement of DNA replication in PCR |
UDG strand cleavage
UDG-mediated strand cleavage is an important tool in molecular biotechnology, allowing for controlled and location-specific cleavage of single- and double-stranded DNA chemically or enzymatically synthesized with single or multiple incorporations of deoxyuridine.
Enzyme | Activity | Application |
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19160, G5010L, EN19 |
Uracil DNA glycosylase (UDG) is involved in base excision repair; UDG creates abasic sites in uracil-containing DNA; degrades uracil-containing DNA; does not excise uracil from RNA or oligonucleotides |
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G5020L |
Thermolabile UDG creates abasic sites in uracil-containing DNA; degrades uracil-containing DNA; gene isolated from a marine bacterium; inactivated by incubation for 10 min at >50°C |
Thermolabile UDG can be used to eliminate PCR “carry over” contamination when dUTP is substituted for dTTP in the primary PCR reaction mix |
Y9180L 10X Uracil Cleavage System Coming soon |
The system consists of two enzyme components, Uracil DNA Glycosylase and Endonuclease VIII. When the enzymes are added sequentially to a reaction where a synthetic DNA fragment contains a deoxyuracil, a single nucleotide gap is generated at the location of the uracil residue |
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Y9080L Endonuclease VIII Coming soon |
Endonuclease VIII functions as both an N-glycosylase (by excising oxidative base lesions) and an AP lyase (by subsequently cleaving the phosphodiester backbone), leaving terminal phosphates at the 5’ and 3’ ends; participates in base excision repair of oxidatively generated DNA damage |
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Discover featured enzymes for enzymatic cleanup and improving yields
FAQs about reducing artifacts and reaction cleanup
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