Enzymes for Molecular Biology
PCR and DNA Amplification
Taqs like it hot and some like it hot from the start
Most in vitro amplification applications use a thermostable enzyme for the polymerase chain reaction (PCR). The most used PCR enzyme is Taq DNA polymerase. Taq DNA polymerase is derived from Thermus aquaticus, an extremely thermophilic eubacterium which grows at temperatures above 70°C. This means that the optimal enzymatic activity of Taq DNA polymerase is expressed at approximately 72°C.
Standard PCR or hot-start?
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