
Detect and discover with footprints and fragments
Enzymes give you the power to discover sites where proteins bind to DNA, to determine transcript abundance, reveal single base substitutions, map exons, visualize apoptosis and measure DNA damage in cells.
Enzyme footprinting characterizes DNA-protein interactions
DNA breaks indicate apoptosis and genotoxicity
RNase A digestion locates base substitutions and maps transcripts
The capacity of enzymes to synthesize or digest nucleic acids, cleave them at specific sites or excise individual bases from a chain makes it possible to apply these enzyme attributes to answer questions through detection, assay and analysis.
Enzyme | Activity | Assay |
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79254 RNase-free DNase (1500 Kunitz units) |
Endonuclease that nonspecifically cleaves DNA to release di-, tri- and oligonucleotide products with 5’-phosphorylated and 3’-hydroxylated ends; acts on ssDNA and dsDNA, chromatin and RNA:DNA hybrids |
DNA-protein interaction analysis (Footprinting assay for DNA-binding proteins) |
X8020L Exonuclease III Coming soon |
Exonuclease that excises bases from duplex DNA in 3’→5’ direction |
DNA-protein interaction analysis (Footprinting assay for DNA-binding proteins) |
Endoribonuclease that degrades ssRNA at C and U residues |
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P7070L |
DNA polymerase that does not require a template to amplify DNA; adds single nucleotides to the 3’-end of any DNA sequence |
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P7260L T7 DNA Polymerase Coming soon |
DNA polymerase with high rate of synthesis and replication fidelity; a two-subunit protein with a polymerase domain and a processivity factor (E. coli thioredoxin) |
In situ labeling of DNA damage (Apoptosis assay) |
P7060L |
DNA polymerase with 5’→3’ synthesis and 3’→5’ exonuclease activities | End-filling 5' protruding ends of DNA fragments with labeled dNTPs |
Y9080L |
Endonuclease VIII functions as both an N-glycosylase (by excising oxidative base lesions) and an AP lyase (by subsequently cleaving the phosphodiester backbone), leaving terminal phosphates at the 5’ and 3’ ends; participates in base excision repair of oxidatively generated DNA damage |
Measurement of DNA damage via single cell gel electrophoresis (Comet assay) |
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