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Enzymes for Molecular Biology

Enzymes for Assays & Detection

Enzymes give you the power to discover sites where proteins bind to DNA, to determine transcript abundance, reveal single base substitutions, map exons, visualize apoptosis and measure DNA damage in cells. 

The capacity of enzymes to synthesize or digest nucleic acids, cleave them at specific sites or excise individual bases from a chain makes it possible to apply these enzyme attributes to answer questions through detection, assay and analysis.

Enzyme Activity Assay
RNase-free DNase
(1500 Kunitz units)
Endonuclease that nonspecifically cleaves DNA to release di-, tri-
and oligonucleotide products with 5’-phosphorylated and
3’-hydroxylated ends; acts on ssDNA and dsDNA, chromatin
and RNA:DNA hybrids
DNA-protein interaction analysis (Footprinting assay for
DNA-binding proteins)
Exonuclease III
Coming soon
Exonuclease that excises bases from duplex DNA in 3’→5’
DNA-protein interaction analysis 
(Footprinting assay for DNA-binding proteins)

RNase A

Endoribonuclease that degrades ssRNA at C and U residues
  • Determining relative or absolute transcript abundance
  • Mapping mRNA termini and intron/exon boundaries
    (RNase protection assay)
  • Mapping single-base mutations in DNA or RNA via
    cleavage at a single base mismatch in an RNA:DNA hybrid

Transferase (TdT)
Coming soon

DNA polymerase that does not require a template to amplify DNA;
adds single nucleotides to the 3’-end of any DNA sequence
  • Adding a homopolymeric tail to 3’-OH DNA ends 
  • Labeling blunt ends of dsDNA breaks (TUNEL)
T7 DNA Polymerase
Coming soon
DNA polymerase with high rate of synthesis and replication
fidelity; a two-subunit protein with a polymerase domain and
a processivity factor (E. coli thioredoxin)
In situ labeling of DNA damage (Apoptosis assay)

Klenow Fragment
Coming soon

DNA polymerase with 5’→3’ synthesis and 3’→5’ exonuclease activities End-filling 5' protruding ends of DNA fragments with labeled dNTPs

Endonuclease VIII
Coming soon

Endonuclease VIII functions as both an N-glycosylase
(by excising oxidative base lesions) and an AP lyase (by
subsequently cleaving the phosphodiester backbone), leaving
terminal phosphates at the 5’ and 3’ ends; participates in base
excision repair of oxidatively generated DNA damage
Measurement of DNA damage via single cell gel
electrophoresis (Comet assay)
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