Enzymes for detection | DNA damage, exon mapping, protein binding

Detect and discover with footprints and fragments

Enzymes give you the power to discover sites where proteins bind to DNA, to determine transcript abundance, reveal single base substitutions, map exons, visualize apoptosis and measure DNA damage in cells.

Enzyme footprinting characterizes DNA-protein interactions

DNA breaks indicate apoptosis and genotoxicity

RNase A digestion locates base substitutions and maps transcripts

The capacity of enzymes to synthesize or digest nucleic acids, cleave them at specific sites or excise individual bases from a chain makes it possible to apply these enzyme attributes to answer questions through detection, assay and analysis.

Enzyme	Activity	Assay
79254 RNase-free DNase (1500 Kunitz units)	Endonuclease that nonspecifically cleaves DNA to release di-, tri- and oligonucleotide products with 5’-phosphorylated and 3’-hydroxylated ends; acts on ssDNA and dsDNA, chromatin and RNA:DNA hybrids	DNA-protein interaction analysis (Footprinting assay for DNA-binding proteins)
X8020L Exonuclease III Coming soon	Exonuclease that excises bases from duplex DNA in 3’→5’ direction	DNA-protein interaction analysis (Footprinting assay for DNA-binding proteins)
RP145, 19101 RNase A	Endoribonuclease that degrades ssRNA at C and U residues	Determining relative or absolute transcript abundance Mapping mRNA termini and intron/exon boundaries (RNase protection assay) Mapping single-base mutations in DNA or RNA via cleavage at a single base mismatch in an RNA:DNA hybrid
P7070L Terminal Deoxynucleotidyl Transferase (TdT) Coming soon	DNA polymerase that does not require a template to amplify DNA; adds single nucleotides to the 3’-end of any DNA sequence	Adding a homopolymeric tail to 3’-OH DNA ends Labeling blunt ends of dsDNA breaks (TUNEL)
P7260L T7 DNA Polymerase Coming soon	DNA polymerase with high rate of synthesis and replication fidelity; a two-subunit protein with a polymerase domain and a processivity factor (E. coli thioredoxin)	In situ labeling of DNA damage (Apoptosis assay)
P7060L Klenow Fragment Coming soon	DNA polymerase with 5’→3’ synthesis and 3’→5’ exonuclease activities	End-filling 5' protruding ends of DNA fragments with labeled dNTPs
Y9080L Endonuclease VIII Coming soon	Endonuclease VIII functions as both an N-glycosylase (by excising oxidative base lesions) and an AP lyase (by subsequently cleaving the phosphodiester backbone), leaving terminal phosphates at the 5’ and 3’ ends; participates in base excision repair of oxidatively generated DNA damage	Measurement of DNA damage via single cell gel electrophoresis (Comet assay)

Discover featured enzymes for assays and detection

Looking for more enzymes?

Subscribe now and be the first to get notified of our entire portfolio, coming soon!

FAQs about enzymes for assays and detection

Do you have any questions?

Need more detailed information about our enzymes?
Starting a new lab? Your nearest sales representative is waiting to hear from you.

Contact us