Cell and gene therapy

Digital PCR enables a range of gene therapy applications, including adeno-associated virus vector genome titer, lentiviral vector copy number measurement, and CAR-T cell therapy development and manufacturing. This is critical when developing effective and reproducible cell and gene therapies while ensuring patient safety.

Viral titer measurement

Discover how QIAcuity dPCR can generate the same level of accuracy and precision in viral titer quantification as the traditional ddPCR method at increased speed and overall higher throughput and scalability. Discover a complete workflow from viral vector lysis to quantification of residual DNA to vector genome titration and genome integrity determination with superior accuracy, reproducibility and speed.

Benefits of using nanoplate dPCR for AAV titration

• Consistent and robust determination of final titer thanks to efficient capsid lysis and residual DNA removal with our specialized kits
• Reduced errors and easy implementation with one protocol with minimal number of manual steps and only 10 minutes of hands-on time
• Higher accuracy and flexibility thanks to multiplexing with as many as 10 single target assays with different dye combinations; option to extend to 12-plex capacity with genes of interest (GOI) assays
• Precise assessment of genome integrity using up to 12 targets simultaneously enabled by our advanced QIAcuity software feature