Analysis of ESR1.
Analysis of ESR1.
High PCR success rate, even with bisulfite converted DNA.
High PCR success rate, even with bisulfite converted DNA.
High Pyrosequencing success rate of predesigned PyroMark CpG Assays.
High Pyrosequencing success rate of predesigned PyroMark CpG Assays.
Ready-to-use methylation assays.
Ready-to-use methylation assays.
Analysis of ESR1. [A] The GeneGlobe interface for PyroMark CpG Assays affords access to multiple targets in CpG islands, which allows the user to pick and choose the particular regions in a CpG island to analyze. [B] Pyrosequencing data show the analysis of two different positions within the CpG island associated with the gene ESR1.
High PCR success rate, even with bisulfite converted DNA. The PCR performance of PyroMark CpG Assays for 310 randomly selected genes was evaluated. A total of 305 assays (98%) resulted in the predicted PCR amplicon, usable as template for subsequent Pyrosequencing.
High Pyrosequencing success rate of predesigned PyroMark CpG Assays. The Pyrosequencing performance of PyroMark CpG Assays for 310 randomly selected genes was evaluated. A total of 305 assays (98%) resulted in the predicted PCR amplicon, usable as template for subsequent Pyrosequencing. From these assays, 281 resulted in successful Pyrosequencing results. Of these successful assays, 229 (75.1%) required no further review by the user, and 52 (17%) were successful after user review. Only 24 (7.9%) evaluated assays failed.
Ready-to-use methylation assays.

The GeneGlobe interface for searching and ordering PyroMark CpG Assays provides access to multiple targets in CpG islands, allowing users to pick and choose the exact regions in a CpG island for analysis. By combining different assays, users can quantify methylation of as many target sites as needed, whether these are consecutive or not.