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qPCR SG Mix (200)
Cat. No. / ID: AM01-020
Uses SG dsDNA-binding dye and is compatible with qPCR instruments that don't need ROX dye.
Components: 2 x 1 mL of 2x qPCR SG Mix, 2 x 1.5 mL of PCR-grade water
Formulation: 2x concentrated mix: TaqNova HS polymerase, dNTPs, 6 mM MgCl2, PCR enhancers, stabilizers, optimized buffer
Storage temperature: -20°C for long-term storage, 2-8°C for up to 1 month after thawing. Does not lose its activity after eight successive thaw cycles. Keep in the dark.
OEM Product
qPCR SG Mix
qPCR SG Mix ROX
qPCR Probe Mix
qPCR Probe Mix ROX
Quantity
200
2000
The qPCR Mixes is intended for molecular biology applications. This product is neither intended for the diagnosis, prevention or treatment of a disease, nor has it been validated for such use either alone or in combination with other products.
Let’s talk custom
Need this product modified? Contact us to get started with tailored OEM solutions.
Features
- Detects low copies of DNA or cDNA targets
- Produces consistent amplification across a wide dynamic range (6 logs)
- Eliminates non-specific amplification (tested up to 45 cycles)
- Detects molecular targets accurately as fast as 30 minutes
- No loss of activity after eight successive freeze cycles
Product Details
qPCR SG and Probe Mixes are ready-to-use, 2x concentrated master mix of TaqNova HS polymerase, which is a mixture of recombinant Taq polymerase expressed in E. coli and a particular monoclonal anti-Taq antibody.
The TaqNovaHS polymerase enables easy set-up of a hot-start PCR reaction at room temperature. The antibody binds reversibly to the enzyme, inhibiting polymerase activity at ambient temperatures. This prevents the extension of non-specifically annealed primers and primer dimers formed at low temperatures during PCR set-up. The antibody is released from the polymerase during the initial DNA denaturation step, thus providing total DNA polymerase activity during standard cycling conditions.
Performance
| Assay | Specification |
|---|---|
| DNase contamination | None detected |
Principle
Precisely optimized buffer components, reliable DNA polymerase and the appropriate selection of PCR enhancers provide a high performance, sensitivity and specificity over a broad dynamic range and give consistent results across all commonly used real-time PCR platforms.
Procedure
Quality Control
qPCR Mix is extensively tested for its performance in different Real-Time PCR assays. It is free of deoxyribonuclease contamination.
Applications
This is used for applications such as:
- qPCR or real-time PCR
- Gene expression analysis
- Mass screening and pathogen detection
- Characterization of genetically modified organisms (GMO)
Resources
Quick-Start Protocols (3)
Safety Data Sheets (1)
Certificates of Analysis (1)