MaXtract High Density

有機溶媒からの核酸のより安全で便利な抽出用

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MaXtract High Density (200 x 2 ml)

Cat. No. / ID:  129056

200 x 2 ml MaXtract High Density Tubes
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$294.00
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Quantity
200 x 2 ml
200 x 1.5 ml
100 x 15 ml
25 x 50 ml
MaXtract High Densityは分子生物学的アプリケーション用であり、疾病の診断、予防、あるいは治療に使用することはできません。

✓ 24/7 automatic processing of online orders

✓ Knowledgeable and professional Product & Technical Support

✓ Fast and reliable (re)-ordering

Features

  • 有機溶媒から核酸をより安全に抽出
  • 汚染物質のキャリーオーバーを低減
  • 高い核酸回収率
  • 便利な核酸回収

Product Details

MaXtract High Densityは、有機溶媒(フェノール/クロロホルムなど)からの核酸抽出を簡素化します。MaXtractゲルは、有機溶媒と核酸を含む水相の間に安定したバリアを形成し、有機溶液、タンパク質、その他の汚染物質のキャリーオーバーを防ぎながら、水相の回収を容易にします。

Performance

MaXtract High Densityは、従来の抽出方法と比較して、30%高い回収率を実現します。タンパク質やその他の汚染物質は、有機相や中間相に移動します。MaXtract High Densityゲルが形成するバリアには十分な耐久性があるため、これら汚染物質はバリアの下に閉じ込められたままになり、水相を除去する際のキャリーオーバーを防ぎます。水相はデカントやピペッティングで除去でき、従来の有機抽出法を使用した場合よりも最大30%高い核酸回収率が得られます。

MaXtract High Densityは、さまざまなサイズのチューブで提供され、100 µlから20 mlまでのサンプル量から抽出できます。

MaXtract High Densityによる相分離
水相フェノール:クロロホルムクロロホルム
<0.5 M NaCl、<1 mg/mlタンパク質はいはい
≥0.5 M NaClはいはい
≥1 mg/mlタンパク質はいはい
プラスミドDNA分離はいはい
ゲノムDNA分離はいはい
RNA分離はいはい

Principle

MaXtract High Densityゲルは、フェノール/クロロホルムなどの溶媒を使用する有機抽出から核酸を安全かつ迅速に回収します(フローチャート「 MaXtract procedure」参照)。
See figures

Procedure

核酸溶液と有機溶媒をMaXtractゲルの入ったチューブに加えるだけです。混合し、遠心分離すると、MaXtract High Densityゲルは安定したバリアを形成し、有機相と水相を分離します。このバリアは有害な有機溶媒と汚染物質を安全に捕捉し、核酸を–含む水相を新しいチューブに簡単にデカントまたはピペットで移すことができます(図「 安全で簡単な核酸抽出」を参照)。2回目の抽出が必要な場合は、チューブの最大容量を超えない限り、同じチューブで抽出できます。

MaXtract High Densityゲルを使用する分離は、水性媒体と有機媒体の密度差に依存します。有機層はゲルや水相よりも密度が高く、ゲルは水相よりも密度が高くなければなりません。

See figures

Applications

MaXtract High Densityは、プラスミドDNAまたは、ゲノムDNA、RNAの分離に非常に適しています。低融点アガロースからのDNAの回収など、特定のアプリケーションに最適化されたプロトコールもご利用いただけます。MaXtract High Densityゲルを用いて抽出した核酸は、数多くのダウンストリームアプリケーションに適しています。

Supporting data and figures

Resources

MSDS (1)
Download Safety Data Sheets for QIAGEN product components.
キットハンドブック (1)
For improved recovery of nucleic acids during organic extraction procedures
クイックスタートプロトコール (1)
MaXtract High Density
PDF (565KB)
Certificates of Analysis (1)
Technical Information and Important Notes (1)
Kit Handbooks (1)
For improved recovery of nucleic acids during organic extraction procedures
Quick-Start Protocols (1)
MaXtract High Density
PDF (565KB)

FAQ

Can phenol/chloroform be placed in the MaXtract tube for extraction at a later time?

Yes. The organic phase can be placed in MaXtract Low and High Density tubes without any problem 3-4 hours before preparation of the samples. Make sure to perform the centrifugation step first, so that the gel collects at the bottom of the tube.

 

FAQ ID -1299
Can MaXtract tubes be autoclaved prior to use?

No. After autoclaving, the MaXtract gel no longer has the same properties. Since the MaXtract tubes are manufactured and filled in a fully automated process, autoclaving is not required.

 

 

FAQ ID -1305
Can DNA isolated with MaXtract tubes be used for downstream applications such as restriction digestion, labeling, blotting, PCR, and automated sequencing?

DNA purified by organic extraction can principally be used for these downstream reactions. It should be noted that phenol, like all organic solvents, alters the active centre of enzymes, thus deactivating them. An ethanol precipitation should therefore generally be carried out prior to enzyme controlled downstream reactions. Potential phenol residue is thereby effectively removed.

The use of MaXtract Low and High Density tubes has the advantage that no recontamination of the sample by organic solvents or proteins occurs.

 

FAQ ID -1303
What factors determine if denatured proteins accumulate underneath, or inside the gel of the MaXtract Low and High Density Tubes?

Generally, this is determined by the density of the denatured proteins and the gel type in the MaXtract Tubes. If proteins show a higher density than the gel, they will accumulate underneath in the organic phase. If they have the same density as the MaXtract gel, they will collect in the gel. The quantity and ratio of phenol:chloroform can also influence the behavior of the proteins.

 

FAQ ID -1312
Do you have a protocol for purification of total RNA from fatty tissues using QIAzol Lysis Reagent and MaXtract High Density?

Yes, we have the following protocols:

  • Purification of total RNA from fatty tissues using QIAzol Lysis Reagent, MaXtract High Density, and the TissueRuptor (RY29).
  • Purification of total RNA from fatty tissues using QIAzol Lysis Reagent, MaXtract High Density, and the TissueLyser (RY30).
  • Purification of total RNA from fatty tissues using the RNeasy Lipid Tissue Mini Kit and MaXtract High Density (RY31).
FAQ ID -1550
Is it possible to use MaXtract for an organic extraction with a mixture of phenol and BCP (1 bromine chlorpropane)?

Yes, this application is possible with both MaXtract Low and High Density Tubes. A sufficient quantity of BCP should be used when using MaXtract High Density, in order to achieve a stable gel phase. BCP increases the density of the organic phase in the same way as chloroform.

 

-3
Does the addition of sample and organic solution to the MaXtract Tubes require a specific pipetting sequence?

No. The functional efficiency of the MaXtract Low and High Density Tubes does not depend on the pipetting sequence.

 

FAQ ID -1307
Can frozen sample material pulverized in liquid nitrogen be directly added to MaXtract tubes?

If organic solvents are added immediately following the filling of MaXtract Low and High Density Tubes with the frozen sample, it should not have any negative effect on the functionality of the tubes.

 

FAQ ID -1301
Can the MaXtract High Density Tube be used with QIAzol to isolate RNA?
FAQ ID -1304
Which density gel type is contained in the yellow or green MaXtract Tubes?

MaXtract High Density gel is found in the yellow tubes, and MaXtract Low Density gel is contained in the green tubes.

 

FAQ ID -1315
Can several extraction steps be performed in the same MaXtract tube?

Yes, if a sufficiently large MaXtract tube has been selected for the extraction it is possible to perform multiple purification steps in this tube. Sample and organic solvent are placed in the MaXtract tube, mixed and centrifuged. Organic solvent is then added again to the aqueous phase separated by the gel, followed by mixing and centrifuging. Following the last extraction step, the sample is transferred to a new tube for storage.

For the purification of samples with high protein content, we recommend using a new MaXtract tube for each extraction step.

 

FAQ ID -1302
When isolating DNA from plant cells using CTAB, should MaXtract Low or High Density be used?

CTAB as a rule increases the density of the aqueous phase. Therefore, MaXtract High Density should be used for the organic extraction of DNA from CTAB samples.

Depending on the amount of CTAB used, the density of the sample can become too high for the MaXtract High Density Tube, leading to a gel barrier above the aqueous phase. In this case, puncture the gel with the tip of a pipette and dilute the aqueous phase with either distilled water or TE buffer. Mix again and then centrifuge. However, we recommend to repeat the separation in a new MaXtract tube.

 

FAQ ID -1308
Can a phenol-chloroform ratio different from 1:1 also be used with MaXtract Low and High Density?

A phenol-chloroform ratio of 1:1 is advantageous for the phase separation when using MaXtract Low and High Density, since the MaXtract interphase has the greatest stability at this ratio.

Methods using a phenol-chloroform ratio of as much as 6:1 are also possible with MaXtract. However, MaXtract can partly precipitate onto the bottom of the tube in this case.

If the phenol-chloroform ratio is greater than 1:1, it is necessary to use MaXtract Low Density Tubes. Note that this MaXtract type is not compatible with applications in which the aqueous phase has a high density (e.g., isolation of plasmid DNA and RNA).

 

FAQ ID -1309
If both MaXtract Low Density and High Density is suitable for an application, which type is preferable to use?

In this case, both MaXtract Low and High Density Tubes can be used with the same success. We principally recommend testing both MaXtract types.

 

 

FAQ ID -1310
Are MaXtract tubes siliconized?

No, MaXtract Low and High Density tubes are not coated in any way.

 

FAQ ID -1298