EZ1 RNA Tissue Mini Kit

プレパック試薬カートリッジで高純度トータルRNA の自動精製

S_1294_9_LS_QF_EZ1_2_RNA_Tissue_Mini_Kit_48

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EZ1&2 RNA Tissue Mini Kit (48)

Cat. No. / ID:  959034

For 48 preps: Reagent Cartridges (Tissue RNA), Disposable Filter-Tips, Disposable Tip-Holders, Sample Tubes (2 ml), Elution Tubes (1.5 ml), Buffer RLT, RNase-free DNase I, RNase-free water
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$674.00
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The name of this kit was changed from "EZ1 RNA Tissue Mini Kit" to "EZ1&2 RNA Tissue Mini Kit" to demonstrate that it is compatible with the new EZ2 Connect instruments as well as the EZ1 Advanced XL. No changes were made to the well-established kit technology.
EZ1 RNA Tissue Mini Kitは分子生物学的アプリケーション用であり、疾病の診断、予防、あるいは治療に使用することはできません。

✓ 24/7 automatic processing of online orders

✓ Knowledgeable and professional Product & Technical Support

✓ Fast and reliable (re)-ordering

Features

  • 最大30 µgのトータルRNA精製
  • 10 mg までの標準的な組織サンプルから信頼できる操作
  • RNaseフリーのセットアップで信頼できる結果
  • 溶出容量が調節可能
  • プログラム済みのICカードを利用して簡単にプロトコールをセットアップ

Product Details

EZ1 RNA Tissue Mini Kit には磁性粒子テクノロジーを用いて溶解しやすい組織サンプル(最高10 mg)からトータルRNAを自動精製するために必要な試薬やプラスチックウェアが全て入っています。プレパックカートリッジ試薬なので、ワークステーションへのセッティングも迅速かつ簡便に行なえます。EZ1 RNA Tissue Mini KitはEZ1 Advanced XLおよびEZ1 AdvancedあるいはBioRobot EZ1で使用可能です。

Performance

EZ1 RNA Tissue Mini Kit で精製したトータルRNAは、収量と高い品質が一定であって再現可能です(図" 効率的な精製および再現性の高い収量" と " 一定した高純度RNA")。

See figures

Principle

EZ1 RNA Tissue Mini Kitでの自動化精製には、EZ1 Advanced XLおよび EZ1 Advanced XL RNA Card、EZ1 Advancedおよび EZ1 Advanced RNA Card、あるいはBioRobot EZ1および EZ1 RNA Cardが必要です。EZ1 cardsにはオペレーターのためのスクリーン上での解説および精製操作用のコマンドが含まれています。

シリカゲルでコーティングされた磁性粒子テクノロジーにより、使いやすいワークステーションを用いて1回のランで1~14サンプルから(EZ1 Advanced XL)あるいは1~6サンプルから(EZ1 AdvancedおよびBioRobot EZ1)の精製が可能です。充填済みの試薬カートリッジとセンサーによるワークステーションドアの自動ロックにより、実験環境からのコンタミのリスクやRNaseによる分解を抑えることができます。自動化精製により再現性の高い結果と、広範囲にわたるスタートサンプル量から直線性のある収量が得られます。広範囲な組織タイプから高品質なトータルRNAを精製する柔軟性を持つため、様々な遺伝子発現解析アプリケーションのためのサンプルのRNA調製が可能です。EZ1 RNA Tissue Mini Kit を使用した場合、RNA収量は、サンプルの重量、組織の種類、細胞の発育状態などの多くの変数に左右されます。

Procedure

組織をまずBuffer RLTで溶解・ホモジナイズします。EZ1 RNA自動化操作に従いEZ1 AdvancedあるいはBioRobot EZ1でRNAがワンステップで精製されます。微量なDNAにも影響をうけるある種のRNAアプリケーションには、インテグレートされているDNase分解ステップによりゲノムDNAを除去します。トータルRNAは200 µl、100 µl、50 µl で溶出可能で、ダウンストリームアプリケーションに即使用可能です。

Applications

EZ1 RNA Tissue Mini Kitを用いて得られた高純度RNAは、次のような多くのアプリケーションに最適です。

  • リアルタイムRT-PCRテクノロジーを用いた遺伝子発現解析
  • マイクロアレイテクノロジーを用いた遺伝子発現解析
  • 腫瘍学研究

Supporting data and figures

Specifications

FeaturesSpecifications
ApplicationsPCR, real-time RT-PCR, microarray
Time per run or per prep40 minutes
TechnologyMagnetic particles
Elution volume50–200 µl
ProcessingAutomated
FormatSample tube
StabilizationYes
For automated processingEZ2 Connect instruments or EZ1 Advanced XL
Main sample typeTissue samples
Sample amount5–10 µg
Yield<30 µg
Purification of total RNA, miRNA, poly A+ mRNA, DNA or proteinRNA

Resources

パンフレット (2)
MSDS (1)
Download Safety Data Sheets for QIAGEN product components.
キットハンドブック (1)
For purification of total RNA from tissue and cells using EZ1 instruments
Safety Data Sheets (1)
Certificates of Analysis (1)
Brochures & Guides (2)
Kit Handbooks (1)
For purification of total RNA from tissue and cells using EZ1 instruments

FAQ

Is training of lab personnel included with the purchase of a BioRobot EZ1?

Operation of the BioRobot EZ1 is so easy that no additional training other than the initial demo is needed. Operating the instrument requires only three major steps:

  • Insert the appropriate EZ1 Protocol Card, switch on the workstation, and, if necessary, select the appropriate protocol
  • Follow the instructions on the liquid-crystal display (LCD), which describes where the sample tubes, elution tubes, filter tips, and EZ1 Reagent Cartridges must be loaded and which indicates when to start the protocol run
  • Collect purified nucleic acids when the protocol run ends

 

FAQ ID -1239
Are the new EZ1 RNA Kits (cat. no. 958034; 959034; 956034) compatible with the previous EZ1 RNA Card (cat. no. 9015590) or EZ1 RNA Universal Tissue Card (cat. no 9016384)?

No, the new EZ1 RNA Kits can only be used with updated protocols programmed on the new EZ1 RNA Card V2.0.

The new card contains all protocols available for the isolation of RNA on the BioRobot EZ1 with the new kits:

Likewise, the former EZ1 RNA Cell Mini Kit (cat. no. 958134), EZ1 RNA Tissue Mini Kit (cat. no. 959134) and EZ1 RNA Universal Tissue Kit (cat. no. 956136) are not compatible with the new EZ1 RNA Card (cat. no. 9015590).

FAQ ID -1025
Do you have a protocol for the isolation of total nucleic acids from animal and human tissues on the BioRobot EZ1?
How can we get our BioRobot EZ1 upgraded when new protocols are launched?

Protocols for nucleic acid purification are supplied on pre-programmed EZ1 Protocol Cards. These protocols provide both on-screen instructions for the operator and operating commands for the workstation. The range of kits and protocols for the EZ1 is continuously expanding to allow nucleic acid purification from a wide range of sample types. Please browse our Supplementary Protocol Section to find specialized EZ1 protocols for a large variety of clinical sample types, in addition to the standard sample types covered in our EZ1 handbooks.

 

FAQ ID -1241
Where can I find information about the worktable setup on the BioRobot EZ1?

Loading of sample tubes, elution tubes, filter-tips, and Reagent Cartridges onto the worktable of the BioRobot EZ1 is intuitive, easy and quick. Details about worktable setup are provided with each new protocol, and are also displayed in the liquid-crystal display (LCD) of the control panel when you start setup of the worktable.

 

FAQ ID -1243
Do the foils sealing the EZ1 Reagent Cartridges have to be manually removed prior to loading the robot?

No, prefilled Reagent Cartridges can be loaded onto the BioRobot EZ1 with their seals. During operation of the instrument, the piercing unit, a row of 6 metal spikes located behind the tip adapters, will puncture the foil of the cartridges, thereby exposing the reagents.

 

FAQ ID -1240
What has to be done to an RNA sample before loading it onto an Agilent Bioanalyzer?

For RNA isolated on the BioRobot EZ1 and BioRobot M48:

The RNA can be directly applied to the Agilent Bioanalyzer, since it is being denatured in the final protocol steps of these isolation procedures.

For RNA prepared with all other QIAGEN RNA Isolation Products:

We recommend to denature the samples in a water bath for 2 min at 70°C, and then place them directly on ice prior to loading them onto the Agilent Bioanalyzer.

 

FAQ ID -528
Do you have a protocol for the isolation of total RNA from paraffin-embedded sections on the BioRobot EZ1?
FAQ ID -976
Do you have a protocol for the isolation of DNA from fungi using the BioRobot EZ1?
Yes, please follow the Supplementary Protocol 'Purification of DNA from fungi using the EZ1 DNA Tissue Kit' (MA35).
FAQ ID -992
How do I safely inactivate biohazardous flow-through material?

Always dispose of potentially biohazardous solutions according to your institution’s waste-disposal guidelines. Although the lysis and binding buffers in QIAamp, DNeasy, and RNeasy kits contain chaotropic agents that can inactivate some biohazardous material, local regulations dictate the proper way to dispose of biohazards. DO NOT add bleach or acidic solutions directly to the sample-preparation waste. Guanidine hydrochloride in the sample-preparation waste can form highly reactive compounds when combined with bleach.
Please access our Material Safety Data Sheets (MSDS) online for detailed information on the reagents for each respective kit.

FAQ ID -12
What disposables are required for nucleic acid isolations on the BioRobot EZ1?

No extra accessories need to be purchased for the BioRobot EZ1, as sample and elution tubes, as well as tips and tip holders are included with each EZ1 DNA or RNA isolation kit.

 

FAQ ID -1246