S_1563_GEF_TLLT_50

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TissueLyser LT

Cat. No. / ID:  85600

Compact bead mill, 100–240 V AC, 50–60 Hz; requires the TissueLyser LT Adapter, 12-Tube (available separately)
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InstrumentAccessories
TissueLyser LT
TissueLyser LT Adapter
TissueLyser LTは分子生物学的アプリケーション用であり、疾病の診断、予防、あるいは治療に使用することはできません。

✓ 24/7 automatic processing of online orders

✓ Knowledgeable and professional Product & Technical Support

✓ Fast and reliable (re)-ordering

Features

  • 同時に最大12 サンプルを破砕
  • 省スペースのコンパクト装置
  • 冷却可能なアダプターにより生体分子の分解を回避
  • すべてのサンプル種で再現性のある結果
  • すべてのワークフローに対応

Product Details

TissueLyser LT は同時に最大12 サンプルを迅速かつ効果的に破砕する小型ビーズミルです。このスループット数は、QIAGEN のスピンカラムキットを用いたサンプル調製の自動化を実現する QIAcubeの処理数に合っています。ステンレススチールあるいはガラスビーズと共にサンプルを2 mlのマイクロ遠心チューブ中で高速振蘯することにより同時破砕およびホモジナイズを実現します。TissueLyser LTは、破砕プロセス中にチューブを保持するTissueLyser LT Adapter(冷却可能)と組み合わせて使用します。付属品として ビーズおよび ビーズディスペンサーがあります。

Performance

TissueLyser LT でヒト、動物、植物の組織、バクテリア、酵母を効果的に破砕した場合、次に行なうDNA、RNA、タンパク質精製操作で再現性のある収量が得られます(図 “  効果的な組織破砕”)。心臓や脳などの溶解困難な組織もTissueLyser LT で破砕することができます。DNA、RNA、タンパク質は破砕後も分解されることなく(図 “ RIN値の高い高純度RNAを精製”、“ どのような解析にも使用可能なインタクトなタンパク質”、“ 動物組織から精製した高品質DNA、“ 植物組織から精製した高品質DNA、および“ 植物組織から精製したインタクトなRNA”)、ダウンストリームアプリケーションで信頼できる解析を行なえます。

TissueLyser LT による破砕は、既に確立されているTissueLyser II の破砕に匹敵します(図 “ 効果的な組織破砕”、“ どのような解析にも使用可能なインタクトなタンパク質”、“ 動物組織からの高品質DNA”、および“ 植物組織からの高品質DNA ”)。

See figures

Principle

ジェノタイピング、遺伝子発現解析、プロテオミクスにおける実験ではDNA、RNA、タンパク質を確実に高収量で得るために生体サンプルを効果的に破砕する必要があります。TissueLyser LTシステムは、生体分子を完全に遊離するため、迅速かつ完璧にサンプルを破砕し、さらにQIAGEN 製品(表 “QIAGEN 破砕システムに対応するQIAGEN 精製用キット”)を用いて容易に精製を行なえるよう、同時にサンプルをホモジナイズします。

QIAGEN はサンプル採取からDNA、RNA、タンパク質の精製および解析までのトータルソリューションを提供しており、TissueLyser LTはその破砕工程を構成しています。TissueLyser LT はQIAGEN のマニュアル用サンプル調製キットに対応し(表 “QIAGEN 破砕システムに対応するQIAGEN精製用キット”)、またミドルスループットのサンプル調製および解析のための 自動化用製品 にも対応します(表 “QIAGENによるミドルスループットの自動化”)。これには、QIAGEN スピンカラムキットの自動化調製用装置であるQIAcube ならびにDNA、RNA のマルチキャピラリーゲル電気泳動自動化装置であるQIAxcel が含まれています。

QIAGEN破砕システムに対応するQIAGEN精製用キット
精製する生体物質 サンプル/ラン QIAGEN キット
RNA 溶解が容易な組織

RNeasy Kits

RNeasy Plus Kits
RNeasy Protect Kits
RNA 繊維性組織 RNeasy Fibrous Tissue Kits
RNA 全タイプの組織 RNeasy Lipid Tissue Kits
RNeasy Universal Tissue Kits
RNA 植物組織 RNeasy Plant Mini Kit
RNA 酵母 RNeasy Kits
RNA バクテリア RNeasy Protect Bacteria Kits
microRNA 全タイプの組織 miRNeasy Kits
DNA ヒト組織 QIAamp DNA Kits
DNA 動物組織 DNeasy Blood & Tissue Kits
タンパク質 組織 Qproteome Mammalian Protein Prep Kit
リン酸化タンパク質 組織 PhosphoProtein Purification Kit
糖タンパク質 組織 Qproteome Glycoprotein Kits
DNA およびRNA 組織 AllPrep DNA/RNA Kits
DNA、RNA、タンパク質 組織 AllPrep DNA/RNA/Protein Mini Kit
QIAGENによるミドルスループットの自動化
装置 目的 スループット
QIAcube DNA、RNA、タンパク質の精製 最大12サンプル/ラン
EZ1 Advanced ヒトサンプルからDNA およびRNA の精製 最大6サンプル/ラン
EZ1 Advanced XL ヒトサンプルからDNA およびRNA の精製 最大14サンプル/ラン
QIAxcel DNAフラグメントおよびRNAの電気泳動解析 最大96サンプル/ラン
QIAgility 反応セットアップ 最大384サンプル/ラン
Rotor-Gene Q リアルタイムPCRとHRM(high-resolution melting)解析 最大100サンプル/ラン
PyroMark Q24 メチル化および突然変異解析 最大24サンプル/ラン

Procedure

TissueLyser LT はTissueLyser LT Adapter をセットして使用します。サンプルとビーズの入ったチューブ(2 ml)12 本までをアダプターにセットし、TissueLyser LT のピストンにしっかりと装着します。ピストンが上下に高速に動作することにより、ビーズがサンプルを打ち砕くと同時にすり潰し、破砕とホモジナイゼーションを一緒に行なうことが可能です。

省スペース型TissueLyser LT(15 x 27 cm)は、ほとんどのラボに設置可能です。各サンプルは個々のチューブ内で安全に密封されるので、TissueLyser LT はクロスコンタミのリスクなしに複数のサンプル破砕を実現します。新鮮あるいは凍結サンプルを破砕する場合は、TissueLyser LT Adapter をドライアイスで前もって冷却することにより核酸やタンパク質の分解を回避できます (図 “ 植物組織からの高品質DNA”および“ 植物組織からのインタクトなRNA ”)。Allprotect Tissue Reagent (DNA、RNA、タンパク質を安定化) あるいは RNAlater RNA Stabilization Reagent (RNA を安定化) で保存した組織では、アダプターを前もって冷却する必要はありません。

See figures

Applications

ランあたり最大12サンプル処理が可能なTissueLyser LTは、ゲノミクス、トランスクリプトミクス、プロテオミクスのアプリケーションに必要な生体情報を獲得する際に、初期工程で理想的なツールです。TissueLyser LTは、2 mlのサンプルチューブを12本までセット可能なTissueLyser LT Adapter中で、動物およびヒト組織、植物組織、バクテリア、酵母を迅速かつ均一に破砕します。サンプル精製はマニュアル、あるいはQIAcube、QIAsymphony SP、QIAxtractor、EZ1 Advanced、EZ1 Advanced XLでの自動化により行なえます。

Supporting data and figures

Specifications

FeaturesSpecifications
寸法Weight: Approx. 7 kg, Width: 150 mm, Depth: 270 mm, Height: 280 mm, The power supply of the TissueLyser LT is compatible with voltages of 100–240 V
Disruption principleHigh-speed shaking of samples in 2 ml microcentrifuge tubes with stainless steel or glass beads
Protocol/main application on this intrumentSample preparation/sample disruption
FeaturesSimultaneous disruption of up to 12 samples. Compact instrument with small footprint. Coolable adapter to prevent biomolecule degradation. Reproducible results with all sample types. Compatible with all laboratory workflows.
TechnologyBead Mill
Kits compatible with instrumentAll kits for purification of DNA and RNA
Typical run time40 sec - 5 min, depending on protocol

Resources

キットハンドブック (3)
For disruption of up to 12 biological samples
For disruption of up to 12 samples on the TissueLyser LT
Safety Data Sheets (1)
Certificates of Analysis (1)
Kit Handbooks (3)
For disruption of up to 12 samples on the TissueLyser LT
For disruption of up to 12 biological samples

FAQ

Are the stainless steel beads magnetic?

Stainless steel beads sold by QIAGEN are not guaranteed to be magnetic. However, you may find some lots of the beads to be magnetic, due to small variations in the chemical compositions (which do not affect the disruptive functions of the beads).

FAQ ID -9180
Can the sample tubes and the TissueLyser LT Adapter be cooled with liquid nitrogen?

No, cooling with liquid nitrogen would make the sample tubes brittle, resulting in breakage of the tubes during the disruption process. Cooling the adapter and sample tubes on dry ice or in a freezer to approx. -80°C is sufficient to prevent the samples from thawing during the disruption process.

 

See:  TissueLyser Virtual Demo for a demonstration of cooling the samples on dry ice before using the TissueLyser LT.

FAQ ID -2549
How long does the sample disruption with the TissueLyser LT take?
Depending on the downstream application and the tissue, the disruption time typically takes 40 sec to 5 min. For more information about the different applications please refer to the TissueLyser LT Handbook.
FAQ ID -2550
Are there any accessories required for sample disruption with the TissueLyser LT?

Yes, disruption and homogenization are achieved through the beating and grinding effect of beads on the sample material as they are shaken together in 2 mL sample tubes. Sample tubes and different beads can be purchased at QIAGEN (see ordering information in the TissueLyser LT Handbook).

 

See:  TissueLyser Virtual Demo for a virtual demonstration of this process.

FAQ ID -2551
Is an O-ring available to seal the lid of the TissueLyser LT Adapter to prevent aerosolization or escape of pathogens during lysis? Can I operate the TissueLyser LT in a laminar air flow hood?

The TissueLyser LT Adapter (69980) is not guaranteed to be airtight and we do not have an O-ring to seal the lid of the adapter.

If you are working with infectious agents, you will need to validate the disruption parameters on your own to make sure the pathogens do not escape outside.

You should find it possible to operate the TissueLyser LT in a laminar air flow hood.


FAQ ID -9116
Do you sell ceramic beads or glass beads for use with TissueLyser II or TissueLyser LT?

We do not sell ceramic beads or glass beads for use with our TissueLyser II or TissueLyser LT instruments. However, we still sell stainless steal beads and tungsten carbide beads. For more information, please go to this link.

FAQ ID -9189
What is the temperature tolerance of TissueLyser LT Adapter? Can I precool the adapter in liquid nitrogen?

TissueLyser LT Adapter is tolerant to temperatures between –78.5°C and +150°C.

Do not freeze the TissueLyser LT Adapter or the sample tubes in liquid nitrogen. The adapter and tubes may break and may cause injury.

However, the insert of the TissueLyser LT Adapter, the sample tubes, and grinding beads can be placed on dry ice for cooling prior to disruption.


FAQ ID -9111
What are the dimensions of the TissueLyser LT?

Height: 280 mm

Width: 150 mm

Depth: 270 mm


FAQ ID -9114
How can I improve DNA yields from very tough tissues using the DNeasy Blood & Tissue Kit or the QIAamp DNA Mini Kit?

Efficient DNA isolation requires thorough sample disruption and digestion.

Although the QIAamp and DNeasy procedures requires no mechanical disruption of the tissue sample, the lysis time will be reduced if the sample is ground in liquid nitrogen or mechanically homogenized in advance. For mechanical homogenization, a rotor–stator homogenizer, such as the QIAGEN TissueRuptor, or a bead mill, such as the QIAGEN TissueLyser, can be used.

To improve digestion of tough tissue samples, Proteinase K incubation at 56°C can be performed overnight. DNA yields may be improved by increasing the amount of Proteinase K or by adding additional proteinase K after several hours of digestion.  

FAQ ID -374
Can I use 3 mm beads with a 5 mm or 7 mm bead dispenser?
Unfortunately, this is not possible. The 3 mm beads are not compatible with the 5 mm or 7 mm TissueLyser Single Bead Dispenser, as this could result in dispensation of multiple beads, and/or the dispenser may get blocked.
FAQ ID -9185
The disruption of my sample worked well with the TissueLyser LT, but I am not satisfied with the RNA yield. What do you suggest?
The RNA yield can be improved by choosing a specialized RNeasy Kit, which is optimally adapted to the starting material (e.g., RNeasy Lipid Tissue Kits for brain tissue or RNeasy Fibrous Tissue Kit for heart).
FAQ ID -2555
My TissueLyser LT is out of warranty and does not work anymore. Do you offer repair?

We are sorry, but we do not offer repair on the TissueLyser LT.


FAQ ID -9112
Can I use Buffer ATL for disruption of my samples with TissueRuptor, TissueLyser II, or TissueLyser LT instruments?

Buffer ATL can produce foam when used in conjunction with the TissueRuptor. You can add 0.5% final volume of Reagent DX (cat. no. 19088) to prevent the formation of foam.

FAQ ID -9143
What sample numbers can be processed on the TissueLyser LT?

Any number of samples between 1 and 12 can be processed on the TissueLyser LT. If processing either 1 sample or 11 samples, load an additional, empty sample tube to balance the TissueLyser LT Adapter. For more information please see the TissueLyser LT Handbook.

 

See: TissueLyser Virtual Demo for a virtual demonstration on how the TissueLyser LT works.

FAQ ID -2547
What tubes do you recommend for the TissueLyser LT Adapter?

We recommend using 2 ml Sample Tubes RB (cat. no. 990381) with our TissueLyser LT.


FAQ ID -9113
Are the beads RNase free or sterile?

We do not guarantee the stainless steel beads to be RNase free. However, when used according to our specifications for disruption of biological samples, no special treatment is needed to make the beads RNase free. This is because our chaotropic lysis buffers will usually inactivate these and RNases contained in the samples themselves.

FAQ ID -9181
Are the stainless beads ready to use? Do I need to pretreat them with acid before use?

Our stainless steel beads and tungsten carbide beads are ready to use.

FAQ ID -9183
What are the dimensions of the TissueLyser LT?

The TissueLyser LT has the following weight and dimensions:

  • Weight: Approx. 7.35 kg (net); approx. 9 kg (gross)
  • Width: 150 mm
  • Depth: 270 mm
  • Height: 280 mm
FAQ ID -2545
I am working with very fibrous and difficult tissues such as cartilage, heart, muscle and skin. Do you have any recommendations for disruption with TissueLyser LT?

When disrupting tough or very tough samples, we recommend using one and two 7 mm stainless steel beads, respectively, instead of one 5 mm stainless steel bead, to guarantee optimal disruption.

Do not exceed the recommended amount of tissue per sample tube. Please refer to the TissueLyser LT Handbook for our recommendations of starting sample amount for purification of DNA, RNA, or proteins from various sample types.

You may need to increase the time and/or intensity of homogenization from what is recommended in the TissueLyser LT Handbook. But please be aware that this may lead to some fragmentation of gDNA.


FAQ ID -9115
What is the typical amount of tissue used for disruption with the TissueLyser LT?
The amount of starting material is dependent on the sample material that should be disrupted. For plant tissues up to 100 mg, and for human or animal tissues 25 to 30 mg, can be disrupted with the TissueLyser LT. When disrupting yeast up to 5 x 10^7 cells, and when disrupting bacteria up to 7.5 x 10^8 cells, can be used. Using more than the recommended amount of starting material will significantly decrease disruption efficiency.
FAQ ID -2552
Can the samples on the TissueLyser LT be protected from degradation of nucleic acids or proteins?

Yes, if using fresh samples that are not stabilized with a stabilization reagent (e.g., RNAprotect Tissue Reagent or Allprotect Tissue Reagent) the TissueLyser LT Adapter can be precooled on dry ice. During the disruption process the adapter will passively cool the samples to prevent heating and degradation of nucleic acids or proteins.

See:  TissueLyser Virtual Demo for a virtual demonstration of this precooling process with the TissueLyser LT.


FAQ ID -2548
Can the TissueLyser LT Adapter be removed for cleaning?

Yes, the TissueLyser LT Adapter can completely be removed from the instrument for cleaning. For more information on how to clean or decontaminate the instrument and the adapter please refer to chapter 6.1 in the TissueLyser LT User Manual on regular maintenance. The TissueLyser LT Adapter must not be autoclaved.

 

See:  TissueLyser Virtual Demo for a virtual demonstration on how the TissueLyser LT Adapter is used 

FAQ ID -2546
Can I use the TissueLyser LT for disrupting bone? Is this instrument powerful enough?

The largest and most efficient beads that can be used with the TissueLyser LT are the 7 mm stainless steel beads. We suggest pre-disrupting teeth or bone into smaller pieces (e.g., using a hammer) and then use the resulting pieces for further disruption in the TissueLyser LT. In this case, we suggest cooling the samples and the adapter on dry ice prior to disruption, rather than disruption in a lysis buffer.

Please note that the adaptor and tubes should not be cooled using liquid nitrogen, as this could lead to breakage of the TissueLyser Adapter and the sample tubes and may cause injury.


FAQ ID -9110
Can I use the 2 ml Sample Tubes RB (cat. no. 990381) with TissueLyser II and TissueLyser LT?

Yes, the 2 ml Sample Tubes RB (cat. no. 990381) can be used with both TissueLyser II and TissueLyser LT.

FAQ ID -9188
What kind of sample tubes are recommended to use with the TissueLyser LT?
The TissueLyser LT has been thoroughly tested with the Sample Tubes RB (2 ml) catalog number 990381. We do not recommend the use of any other tubes with the instrument.
FAQ ID -2553
How to clean the stainless steel beads?

Stainless steel beads can be reused for DNA extractions with DNeasy Plant Kits. Used beads can be recovered from cell-debris and cleaned using the procedure below:

  1. Close the cap of the 2 ml microtube and briefly vortex to dislodge the bead and pellet from the bottom of the tube. If using grinding jars for the disruption of large sample volumes, skip to step 2.
  2. Empty the contents of the tubes/jars into a sieve and rinse the beads thoroughly with water.
  3. Incubate beads in 0.4 M HCl for 1 min at room temperature (15–25°C) to degrade any DNA and avoid cross-contamination in future preparations.
  4. Rinse beads thoroughly with distilled water to remove the HCl.
  5. Dry beads before use.

You can also find these instructions in Appendix B of the DNeasy Plant Handbook.

FAQ ID -9179
What size bead should I use for tougher samples with the TissueLyser LT?

In general, disruption works very well using one stainless steel bead with a diameter of 5 mm. However, for tougher samples we recommend the use of two 7mm stainless steel beads to optimize the effectiveness of disruption.

 

See:  TissueLyser Virtual Demo for a demonstration on how these beads are used with the TissueLyser LT.

FAQ ID -2554
Do you sell the 3 mm stainless steel beads?

We do not sell 3 mm stainless steel beads for use with our TissueLyser instruments.

FAQ ID -9187