QIAamp DNA Kits for DNA Extraction

Name: QIAamp DNA Kit | ゲノムDNA分離 | QIAGEN
Brand: Qiagen

ゲノムDNA、ミトコンドリアDNA、寄生虫DNA、ウイルスDNAの分離に最適

✓ オンライン注文による24時間年中無休の自動処理システム

✓ 知識豊富で専門的な製品&テクニカルサポート

✓ 迅速で信頼性の高い（再）注文

QIAamp DNA Micro Kit（50）

カタログ番号 / ID. 56304

DNAプレップ50回分：QIAamp MinElute Columns50本、プロテイナーゼK、担体RNA、バッファー、Collection Tubes（2 mL）

￥43,000

キット付属品

QIAamp DNA Kit

QIAamp DNA Accessory Set

カラムタイプ

Micro

Mini

Mini QIAcube

QIAamp DNA Kitは分子生物学的アプリケーション用であり、疾病の診断、予防、あるいは治療に使用することはできません。

✓ オンライン注文による24時間年中無休の自動処理システム

✓ 知識豊富で専門的な製品&テクニカルサポート

✓ 迅速で信頼性の高い（再）注文

特徴

高品質DNAの迅速な精製
有機溶媒による抽出もアルコールの沈殿も不要
一貫性があり、高収量
汚染物質や阻害物質の完全な除去
QIAcube Connectを使った1～12サンプルの自動精製

製品詳細

QIAamp DNA Kitは、組織、スワブ、CSF、血液、体液、尿由来の洗浄細胞からシリカ膜ベースの核酸精製を可能にします。また、少量の新鮮または冷凍された血液、組織、乾燥した血痕からゲノムDNAおよびミトコンドリアDNAを精製できます。組織は酵素で溶解されるため、機械的な均質化は必要ありません。QIAcube Connectと専用のQIAamp DNA Mini QIAcube Kitを使用すれば、1～12サンプルのDNA精製を自動化できます。また、QIAamp DNA Micro KitおよびQIAamp DNA Mini Kitを使用したDNA精製もQIAcube Connectで自動化することが可能です。

QIAamp DNA Mini標準プロトコールは、TRACKMAN Connected systemを使って実行することもできます。

パフォーマンス

QIAamp DNA Micro Kitは、シリカベース膜の選択的結合特性と20～100 µLに対応可能な柔軟な溶出量とを兼ね備えています。QIAamp DNA Micro Kitを使って精製したDNAには、タンパク質、ヌクレアーゼなどの不純物が含まれておらず、real-time PCR（図「少量のサンプルからのDNAの効果的な精製」を参照）やレーザー顕微解剖（LMD）PCR（「レーザー顕微解剖PCR」を参照）のような高感度のダウンストリームアプリケーションに適しています。精製DNAは、ショートタンデムリピート（STR）ジェノタイピング、一塩基多型（SNP）ジェノタイピング、またはファーマコゲノミクスにも使用できます。

QIAamp DNA Mini Kitは、高速スピンカラム法または真空プロトコールを用いることで、組織からのDNA分離を簡略化し、最大50 kbの長さのDNAを回収します（「最大50 kbのゲノムDNAの精製」を参照）。この長さのDNAは完全に変性し、最も高い増幅効率を示します。核酸またはDNAの収量は、使用する出発物質に依存します（表「QIAamp DNA Mini Kitの標準的収量」を参照）。QIAamp DNA Mini Kitを用いて精製したDNAは、PCR、定量real-time PCR、サザンブロッティング、SNPおよびSTRジェノタイピング、ファーマコゲノミクス研究など、さまざまなダウンストリームアプリケーションに使用できます。

サンプル	量	核酸収量の合計（µg）*	DNA収量（µg）†
血液	200 µL	4～12	4～12
軟膜	200 µL	25～50	25～50
細胞	10⁶	20～30	15～20
肝臓	25 mg	60～115	10～30
脳	25 mg	35～60	15～30
肺	25 mg	25～45	5～10
心臓	25 mg	15～40	5～10
腎臓	25 mg	40～85	15～30
脾臓	10 mg	25～45	5～30

QIAamp DNA Mini QIAcube Kitを使用することで、最大50 kbのDNAを回収できます。この長さのDNAは完全に変性し、最も高い増幅効率を示します。精製されたDNAは、以下のようなさまざまなアプリケーションに使用できます

ウイルス研究
細菌研究
菌研究
がん研究
ヒト遺伝子検査研究
父子鑑定
法医学的分析

QIAamp DNA Mini QIAcube Kitは、高速スピンカラム法を用いることで組織からのDNA分離を簡略化します。キットには、あらかじめQIAampスピンカラムと溶出チューブが搭載されたローターアダプターが含まれており、時間と手間を大幅に節約できます（図「時間と手間を大幅に節約」を参照）。

原理

QIAamp DNA精製プロセスでは、DNAはQIAamp MinEluteまたはQIAampシリカゲル膜に特異的に結合し、汚染物質は通過します。フェノール–クロロホルム抽出は必要ありません。2価カチオンやタンパク質などのPCR阻害物質は、2回の効率的な洗浄工程で完全に除去され、純粋なDNAのみが残り、水またはキット付属のバッファーに溶出されます。

QIAamp DNA技術は、ヒト組織サンプルから、PCRやブロッティングにすぐに使用できるゲノムDNA、ミトコンドリアDNA、細菌DNA、寄生虫DNA、ウイルスDNAの抽出を可能にします。

QIAampサンプル調製技術は正式にライセンスを取得しており、QIAampで精製された核酸は、特許侵害の懸念なく、あらゆる分子アッセイやダウンストリームアプリケーションに使用できます。

操作手順

最適化されたバッファーとQIAamp DNA Mini Kitの酵素でサンプルを溶解し、核酸を安定化させ、QIAamp膜に対する選択的DNA吸着を強化します。アルコールを加え、溶解物をQIAampスピンカラムにロードします。

洗浄バッファーを使用して不純物を除去すると、高純度ですぐに使えるDNAが水または低塩バッファーに溶出します。

組織は酵素的に溶解されるため、機械的均質化は必要ありません。便利なスピンカラムを使用したプロセスにより、実際の調製時間はわずか20分で済みます（溶解時間はサンプルの種類によって異なります）。

サンプルはマイクロ遠心機、または血液やその他の体液を処理する場合はQIAvac 24 Plusを使用して処理できます。また、精密な溶解プロセスを採用しているため、QIAamp DNA Mini Kitは細菌や寄生虫からのゲノムDNA精製に最適です。

ゲノムDNA精製をQIAcubeで自動化することで、実際の操作時間をさらに短縮することができます。QIAamp DNA Accessory Set Aには、QIAcubeと12 x QIAamp DNA Mini Kit（50）を使用して、ゲノムDNA、ミトコンドリアDNA、細菌DNA、寄生虫DNA、ウイルスDNAを分離するために必要な追加のバッファーと試薬が含まれています。

真空プロトコール

血液やその他の体液は、遠心分離ではなく真空法で処理することができ、これによりDNA精製の速度と利便性が大幅に向上します。QIAamp Miniスピンカラムは、QIAvac 24 PlusマニホールドにVacValveおよびVacConnectorを使用して設置されています。

サンプルの流量が著しく異なる場合は、一貫した真空状態を確保するためにVacValvesを使用する必要があります。使い捨てのVacConnectorsは、クロスコンタミネーションを防止するために使用されます。さらに、VacConnectorsを使用することで、QIAvac 6SにQIAvac Luer Adaptersを取り付けてQIAampスピン処理を実施することも可能です。

QIAcube ConnectによるDNA分離の自動化

専用のQIAamp DNA Mini QIAcube Kitは、QIAcube機器で使用するために、あらかじめローターアダプターにスピンカラムと溶出チューブを搭載しており、ローターアダプターの誤搭載によるエラーのリスクを防ぎます。この専用キットはQIAcubeの要件に合わせて設計されており、廃棄物の削減にも寄与します。

QIAcube Connectにはシンプルなプロトコール（溶解、結合、洗浄、溶出）と自動化されたプロトコールが搭載されており、ユーザーが行う操作は最小限に抑えられています。

アワードを受賞したQIAcube Connectは、QIAGENスピンカラムの処理に最新技術を活用し、自動化された低スループットのサンプル調製をラボのワークフローにシームレスに組み込むことができます。精製プロセスのすべての工程は完全に自動化され、1回のランで最大12サンプルを処理できます。QIAcube Connectと専用のQIAamp DNA Mini QIAcube Kitを組み合わせることで、高速で簡単、かつ便利なDNA精製が可能となります。

アプリケーション

QIAamp DNA Micro Kitは、少量の血液、乾燥血液スポットカード、尿、レーザーマイクロダイセクションによる切片といった小さい組織サンプルなど、広範なサンプルの処理に適しています。

QIAamp DNA Mini Kitは、筋肉、肝臓、心臓、脳、骨髄などのよく使用されるヒト組織やスワブ（頬粘膜、眼、鼻腔、咽頭、その他）、CSF、血液、体液、尿由来の洗浄細胞からのDNA精製に最適です。組織は最大25 mg、液体は最大200 µLを20分で精製し、50～200 µLを溶出可能です。

QIAamp DNA Mini QIAcube Kitは、QIAcube Connectを使用して、ゲノムDNA、ミトコンドリアDNA、寄生虫DNA、ウイルスDNAの自動分離を行うためのキットです。サンプルの由来には、組織、頬粘膜スワブ、CSF、尿由来の洗浄細胞などがあります。

QIAamp DNA Kitの比較
特徴	QIAamp DNA Micro Kit	QIAamp DNA Mini Kit
アプリケーション	Real-time PCR、STR解析、LMD-PCR	PCR、サザンブロッティング
溶出量	20～100 µL	50～200 µL
フォーマット	スピンカラム	スピンカラム
主なサンプルタイプ	全血	全血、組織、細胞
処理	手動（遠心分離または真空分離）	手動（遠心分離または真空分離）
トータルRNA、miRNA、 poly A+ mRNA、DNA、タンパク質の精製	ゲノムDNA、ミトコンドリアDNA	ゲノムDNA、ミトコンドリアDNA、細菌DNA、寄生生物DNA、ウイルスDNA
サンプル量	1～100 µL	200 µL/25 mg/5 x 10⁶
技術	シリカテクノロジー	シリカテクノロジー
ランあたり、または調製あたりの時間	30分	20分
収量	3 µg未満	4～30 µg

裏付けデータと数値

QIAamp DNA Mini Kit (細胞)を使ったときの典型的な大きさの分布

QIAamp DNA Mini Kitを使用して細胞から得られたDNAの典型的なサイズ分布です。サンプルはFemto Pulse Systemで分析され、信号は最大ピークに基づいて正規化されました。

リソース

For purification of genomic DNA from small volumes of blood, dried blood spots, swabs, forensic case work samples, chewing gum, urine, tissues, laser-microdissected tissues; For cleanup of genomic DNA

Second edition — innovative tools

Download Safety Data Sheets for QIAGEN product components.

The protocol can be used for fresh or frozen semen samples with equal efficiency. Frozen samples must be thawed thoroughly before use. Please note that lysis time will vary depending on the size and density of the source material.

These procedures have been used successfully for isolation of genomic DNA from Aspergillus and Candida species, from both fungal cultures and blood.

This protocol has only been tested with ‘soft’ tissues (e.g., liver, spleen, thymus, heart, kidney, and brain) and may not work with ‘hard’ tissues (e.g., bone, teeth, and skin).

For parallel preparation of genomic, bacterial, or viral DNA or viral RNA from more than 24 samples, we recommend using QIAamp® Spin Columns with 4-6 ml collection tubes. Any tube with an inner diameter of 9-10 mm (outer diameter 11-12 mm) is suitable.

For QIAamp DNA Mini Kit (50) plus QIAamp DNA Accessory Set A, cat. no. 1048145, or QIAamp DNA Mini Kit (250) plus QIAamp DNA Accessory Set B, cat. no. 1048146

よくある質問

For DNA isolation using the QIAamp DNA Mini, or the DNeasy Blood & Tissue Kit, we recommend using carrier DNA when expected yields are below 10 ng. If possible, carrier DNAs such as poly-dA, poly-dT or poly-dA:dT should be used. Other carrier DNAs such as herring sperm DNA may interfere with subsequent PCR by binding primers nonspecifically.

Please note that poly-dA may interfere with oligo-dT primers, and, in this case, a different carrier DNA should be used. The concentration of carrier DNA should be at least 10 µg/ml. Optimal amounts need to be determined empirically for each application. The size distribution of carrier DNAs is typically in the range of 100 bp to 10 kb.

FAQ-100

Even though we have not tested this, we assume that both the QIAamp DNA Micro Kit and the AllPrep DNA/RNA Mini Kit will work to isolate DNA from mitochondria separated with the Qproteome Mitochondria Isolation Kit.

FAQ-1188

Always dispose of potentially biohazardous solutions according to your institution’s waste-disposal guidelines. Although the lysis and binding buffers in QIAamp, DNeasy, and RNeasy kits contain chaotropic agents that can inactivate some biohazardous material, local regulations dictate the proper way to dispose of biohazards. DO NOT add bleach or acidic solutions directly to the sample-preparation waste. Guanidine hydrochloride in the sample-preparation waste can form highly reactive compounds when combined with bleach.
Please access our Material Safety Data Sheets (MSDS) online for detailed information on the reagents for each respective kit.

FAQ-12

Bisulfite conversion of unmethylated cytosines into uracils with the EpiTect Bisulfite Kit works on DNA irrespective of the source organism. The DNA template needs to be of high purity for efficient conversion. We recommend to use genomic DNA extracted with our DNA isolation kits for clinical or animal and plant samples as a template for the EpiTect Bisulfite Kit.

FAQ-1209

Yes. We have used the QIAamp DNA Blood Mini Kit to purify DNA fragments as small as 168 base pairs. Our product profile for this kit shows a picture of the apoptotic banding pattern obtained after storage of blood samples at 4°C for extended periods of time prior to isolating DNA.

FAQ-149

Yes, please follow the Supplementary Protocol 'Purification of REPLI-g amplified DNA using the QIAamp DNA Mini Kit' (RG14).

FAQ-1545

Yes, please follow the User-Developed Protocol 'Purification of genomic DNA from cultured cells using the QIAamp DNA Micro Kit' (QA43).

Note: The maximum amount of cells that can be used with this protocol has not been thoroughly tested. However, we would suggest using no more than 1 x 10⁶ cells.

FAQ-1547

Dedicated QIAcube kits are the RNeasy Mini QIAcube Kit, QIAamp DNA Mini QIAcube Kit , the QIAamp DNA Blood Mini QIAcube Kit, and the QIAamp Viral RNA Mini QIAcube Kit. In addition to these kits are the QIAamp PowerFecal Pro DNA QIAcube Kit, the DNeasy PowerSoil Pro QIAcube Kit using Power technology, and the DNeasy Blood & Tissue QIAcube Kit.

FAQ-2337

Alcohol precipitation is commonly used for concentrating, desalting, and recovering nucleic acids. Since less alcohol is required for isopropanol precipitation, this is the preferred method for precipitation of DNA from large volumes. In addition, isopropanol precipitation can be performed at room temperature, which minimizes co-precipitation of salt that interferes with downstream applications.

Procedure

Adjust the salt concentration, for example, with sodium acetate (0.3 M, pH 5.2, final concentration) or ammonium acetate (2.0–2.5 M, final concentration).
Add 0.6–0.7 volumes of room-temperature isopropanol to the DNA solution and mix well.
Centrifuge the sample immediately at 10,000–15,000 x g for 15–30 min at 4°C
Carefully decant the supernatant without disturbing the pellet.
Wash the DNA pellet by adding 1–10 ml (depending on the size of the preparation) of room-temperature 70% ethanol. This removes co-precipitated salt and replaces the isopropanol with the more volatile ethanol, making the DNA easier to redissolve.
Centrifuge at 10,000–15,000 x g for 5–15 min at 4°C.
Carefully decant the supernatant without disturbing the pellet.
Air-dry the pellet for 5–20 min (depending on the size of the pellet).
Redissolve the DNA in a suitable buffer.

Tip: Use a buffer with a pH of 7.5–8.0, as DNA does not dissolve easily in acidic buffers. Often distilled water can have an acidic pH. The addition of EDTA protects the DNA from DNase digestion.

Tip: High-molecular-weight DNA, such as genomic DNA, should be redissolved very gently to avoid shearing. If the DNA pellet does not dissolve easily, heat at 55°C for 1–2 h with gentle shaking.

FAQ-2953

If you have optimized the lysis conditions for a specific sample type, you can lyse the sample in your lysis buffer, and follow the Supplementary Protocol 'Purification of total DNA from crude lysates using the DNeasy Blood & Tissue Kit' (DY15), or the corresponding protocol in the Appendix of the QIAamp DNA Mini Kit and QIAamp DNA Blood Mini Kit Handbook.

FAQ-303

Add 1/10 volume of 3 M Na-Acetate pH 5.2, and 2 to 2.5 volumes of ice-cold 100% ethanol to the DNA sample
Mix, and store at –20°C for at least 1 h to precipitate the DNA
Recover the precipitated DNA by centrifugation at full speed in a microcentrifuge for 15–20 min
Pour off the ethanol and wash the pellet twice with room-temperature 70% ethanol
Allow the DNA pellet to air-dry
resuspend the DNA in a suitable volume of sterile TE buffer or distilled water

FAQ-305

QIAGEN Protease is inactivated by incubation at 70°C for 15 minutes.

To our knowledge, Proteinase K cannot be completely heat-inactivated. Even when incubating at 95°C for 10 minutes, some enzymatic activity remains. This will not negatively affect the QIAamp Procedure, since the enzyme will be efficiently removed by the wash steps in the protocols.

FAQ-315

Fragments of approximately 200 bp can be isolated with good recovery. Smaller fragments can also be isolated but the recovery will be reduced with decreasing fragment lengths.

FAQ-3212

QIAGEN Proteinase K is stable for up to 1 year after delivery when stored at room temperature. To prolong the shelf-life of Proteinase K, storage at 2–8°C is recommended.

FAQ-3447

In QIAGEN labs, we have isolated DNA from a minimum of 1000 cells or 1 ul of whole blood sample. The expected yield from 1 ul of whole blood with 4000-7000 leukocytes is 30 ng of genomic DNA. The expected yield of genomic DNA from a single eukaryotic cell is 6 pg. However, please bear in mind that for these small quantities, we would recommend the QIAamp DNA Micro kit instead.

FAQ-3516

The size of DNA obtained with QIAamp DNA Mini and QIAamp Blood Mini kit ranges between 100 bp and 50 kb, with 30 kb fragments predominating. The 30 kb fragments are a good starting point for most of the library preparations. Furthermore, the purified DNA is free of protein, nucleases, and other contaminants and inhibitors, and therefore is suitable for NGS.

FAQ-3518

A vial of Protease with 7.5 Anson units (AU) contains approximately 167 mg protein (45 mAU/mg protein). If the content of the vial is diluted in 7 ml, the concentration would roughly be 24 mg/ml.

FAQ-3520

The MinElute spin columns included in the following kits should be stored at 2–8°C upon arrival: AllPrep DNA/RNA Micro, EpiTect Fast DNA Bisulfite, EpiTect Fast FFPE Bisulfite, EpiTect Fast LyseAll Bisulfite, EpiTect Plus DNA Bisulfite, EpiTect Plus FFPE Bisulfite, EpiTect Plus LyseAll Bisulfite, exoRNeasy Serum/plasma Maxi, exoRNeasy Serum/Plasma Midi, GeneRead DNA FFPE, GeneRead rRNA Depletion, GeneRead Size Selection, MinElute Gel Extraction, MinElute PCR Purification, MinElute Reaction Cleanup, miRNeasy FFPE, miRNeasy Micro, miRNeasy Serum/Plasma, QIAamp DNA FFPE, QIAamp DNA Investigator, QIAamp DNA Micro, QIAamp MinElute Media, QIAamp MinElute Virus Spin, QIAamp MinElute Virus Vacuum, RNeasy FFPE, RNeasy Micro, RNeasy Plus Micro.

Short-term storage (up to 4 weeks) at room temperature (15–25°C) does not affect the performance. However, for optimal performance and quality, storage temperature should not exceed 25°C.

FAQ-3560

Efficient DNA isolation requires thorough sample disruption and digestion.

Although the QIAamp and DNeasy procedures requires no mechanical disruption of the tissue sample, the lysis time will be reduced if the sample is ground in liquid nitrogen or mechanically homogenized in advance. For mechanical homogenization, a rotor–stator homogenizer, such as the QIAGEN TissueRuptor, or a bead mill, such as the QIAGEN TissueLyser, can be used.

To improve digestion of tough tissue samples, Proteinase K incubation at 56°C can be performed overnight. DNA yields may be improved by increasing the amount of Proteinase K or by adding additional proteinase K after several hours of digestion.

FAQ-374

The expected DNA yields from different tissues using the QIAamp DNA Mini Kit are as follows:

Sample	Nucleic acid yield without RNase A treatment (ug)	DNA yield with RNase A treatment (ug)
Blood (200 ul)	4–12	4–12
Buffy coat (200 ul)	25–50	25–50
Cultured cells (5 x 10e6)	20–30	15–20
Liver (25 mg)	60–115	10–30
Brain (25 mg)	35–60	15–30
Lung (25 mg)	8–20	5–10
Heart (25 mg)	25–45	5–10
Kidney (25 mg)	40–85	15–30
Spleen (10 mg)	25–45	5–30

DNA was purified with QIAamp Kits following standard protocols.

FAQ-45

When purifying small amounts of DNA using silica technology, the addition of carrier RNA or DNA enhances the recovery of DNA. Carrier prevents the small amount of target nucleic acid present in the sample from being irretrievably bound. Other typical “precipitation” carriers, such as glycogen, cannot be used. The carrier used must be a nucleic acid, and of a large enough size (>200nt) to bind to the silica membrane.

FAQ-473

The initial centrifugations are performed at 6000 x g simply to reduce centrifuge noise. The final spin with the ethanolic wash buffer and the optional drying spin are both done at full speed to enhance ethanol drying from the spin column. Earlier centrifugation steps can also be performed at full speed, and this will not negatively affect DNA yield or quality.

FAQ-474

Using QIAGEN Genomic-tips

Protocol for DNA cleanup using Genomic-tips can be found in the 'Special Applications' section of the QIAGEN Genomic DNA Handbook.

Using QIAamp DNA Micro kit

Up to 10ug of gDNA can be cleaned using the cleanup protocol in the QIAamp DNA Micro Handbook.

Using Gentra Puregene Reagents

Gentra Puregene Handbook has protocols for removal of proteins and RNA from purified gDNA in Appendix C and Appendix D respectively.

FAQ-618

The expected yield of genomic DNA isolated from bacteria with the DNeasy Blood & Tissue Kit or the QIAamp DNA Mini Kit is approximately 10 ug of DNA per 2x 10⁹ bacterial cells. Note that yields of genomic DNA will vary depending on bacterial strain, quality of the starting material, growing conditions, and the amount of material processed.

FAQ-632

Tissue is more difficult to lyse than cells in a blood sample. Therefore an additional incubation step in buffer ATL containing Proteinase K is included in the Tissue Protocol to achieve complete sample lysis. Blood cells will directly lyse by incubation in Buffer AL containing QIAGEN Protease. Buffer AL is required in both protocols to ensure appropriate conditions for DNA binding to the silica membrane. For further details on the protocols, please refer to the QIAamp DNA Mini and QIAamp DNA Blood Mini Kit Handbook.

FAQ-633

Small amounts of RNA and DNA may be difficult to measure spectrophotometrically. Fluorometric measurements, or quantitative RT-PCR and PCR are more sensitive and accurate methods to quantify low amounts of RNA or DNA.

Fluorometric measurements are carried out using nucleic acid binding dyes, such as RiboGreen® RNA Quantitation Reagent for RNA, and PicoGreen® DNA Quantitation Reagent for DNA (Molecular Probes, Inc.).

FAQ-728

The composition of Buffer AE is:

10 mM Tris-Cl
0.5 mM EDTA; pH 9.0.

FAQ-730

EDTA chelates divalent cations which are required for nuclease activity. While the genomic DNA (gDNA) extracted using QIAGEN products, should not have any nuclease activity, it is possible to introduce nucleases during repeated long-term access of the DNA. EDTA helps to prevent any nuclease activity introduced after the genomic DNA extraction procedures.

However, if the gDNA is stored frozen at -20oC or -80oC, nuclease activity is much reduced. It may be possible to leave EDTA out of the storage buffer without negative consequences when samples are kept under these conditions, and when repeated freeze-thaw cycles are avoided.

We do recommend however that gDNA be stored in a neutral to a slightly basic buffered solution (e.g. 10 mM Tris-Cl pH 8.5 to 9.0) to prevent DNA degradation by acid hydrolysis. Note that deionized water mostly has an acidic pH.

FAQ-754

QIAGEN Proteinase K is a subtilisin-type protease, which cleaves at the carboxyl side of hydrophobic, aliphatic and aromatic amino acids. It is particularly suitable for short digestion times. It possesses a high specific activity over a wide range of temperatures and pH values with substantially increased activity at higher temperature. Soluble calcium is not essential for enzymatic activity. This means that EDTA, which is used to inhibit Mg2+-dependent enzymes such as nucleases, will not inhibit Proteinase K activity.

QIAGEN Protease is a broad-specificity Serine protease with high activity, cleaving preferentially at neutral and acidic residues. It is an economical alternative to Proteinase K for isolation of native DNA and RNA from a variety of samples.

FAQ-761

Yes, we have the following protocols:

Isolation of genomic DNA from compact bone using the QIAamp DNA Mini Kit (QA02)
Isolation of genomic DNA from compact bone using the DNeasy Blood & Tissue Kit (DY01)
Purification of genomic DNA from bones using the QIAamp DNA Micro Kit (QA39)
Purification of archive-quality DNA from bone fragments using the Gentra Puregene Tissue Kit (PG38).

FAQ-908

Yes, we have the following protocols:

Isolation of genomic DNA from sperm using the QIAamp DNA Mini Kit; protocol 1 (QA03), long procedure
Isolation of genomic DNA from sperm using the QIAamp DNA Mini Kit; protocol 2 (QA04), short procedure
Purification of total DNA from animal sperm using the DNeasy Blood and Tissue kit; protocol 1 (DY02), long procedure
Purification of total DNA from animal sperm using the DNeasy Blood and Tissue kit; protocol 2 (DY03), short procedure
Purification of DNA from epithelial cells mixed with sperm cells using the QIAamp DNA Micro Kit (QA40).

FAQ-909

Yes, we have the following protocols:

Isolation of genomic DNA from fungi (culture and blood) using the QIAamp DNA Mini Kit (QA09).
Isolation of genomic DNA from plants and filamentous fungi using the QIAGEN Genomic-tip (QG08).

FAQ-911

Yes, please follow either of the User-Developed Protocols:

'Acetyl Cysteine (NALC) treatment of viscous samples' (QA13). This protocol is for use with the QIAamp DNA Mini Kit.
'Acetyl Cysteine (NALC) treatment of viscous samples using the DNeasy Blood & Tissue Kit' (DY06).

FAQ-913

Yes, please follow the User-Developed Protocol 'Detection of Bordetella pertussis DNA by PCR' (QA14). The procedure is for use with the QIAamp DNA Mini Kit. Please contact Technical Service for this protocol.

FAQ-914

Yes, we have the following protocols:

Isolation of genomic DNA from saliva and mouthwash using the QIAamp DNA Mini Kit; vacuum procedure (QA19v)
Isolation of genomic DNA from saliva and mouthwash using the QIAamp DNA Mini Kit; spin procedure (QA19s)
Isolation of genomic DNA from saliva using the DNeasy Blood & Tissue Kit; spin procedure (DY07)

FAQ-917

Yes, please follow either of the User-Developed Protocols:

'Isolation of DNA from soft tissues using the TissueLyser and QIAamp DNA Mini Kit' (QA31), for use with the QIAamp DNA Mini Kit
Purification of total DNA from soft tissues using the TissueLyser and the DNeasy Blood & Tissue Kit' (DY11), for use with the DNeasy Blood & Tissue Kit

FAQ-924

Yes, please follow the User-Developed Protocol 'Purification of DNA from epithelial cells mixed with sperm cells using the QIAamp DNA Micro Kit' (QA40).

FAQ-926

TB Management

Transplant

Infectious Disease

Oncology

Sexual & Reproductive Health

Sample Processing

Solutions for Laboratory-Developed Tests

QIAamp DNA Kits for DNA Extraction

ゲノムDNA、ミトコンドリアDNA、寄生虫DNA、ウイルスDNAの分離に最適

QIAamp DNA Micro Kit（50）

特徴

製品詳細

パフォーマンス

原理

操作手順

アプリケーション

裏付けデータと数値

QIAamp DNA Mini Kit (細胞)を使ったときの典型的な大きさの分布

リソース

よくある質問