Which EpiTect Bisulfite Plus kit do you recommend for blood samples, cells, and tissues?
FAQ ID -2411

The EpiTect Plus LyseAll Bisulfite Kit is recommended for whole blood as well as for cells. The kit includes an innovative lysis buffer for optimized lysis of up to 10E5 cells and up to 20µl whole blood. It also facilitates binding of the DNA to ensure that even small amounts of DNA are bound with high efficiency, thus reducing loss of DNA due to the DNA purification step.

For tissues:e

A) For fresh or frozen tissue the EpiTect Plus LyseAll Bisulfite Kit should be used. The kit includes an innovative lysis buffer for optimized lysis of tissue samples. It also facilitates binding of the DNA to ensure that even small amounts of DNA are bound with high efficiency, thus reducing loss of DNA due to the DNA purification step.

B) For FFPE tissue we strongly recommend the EpiTect Plus FFPE Bisulfite Kit. It includes specialized buffers for efficient deparaffinization and lysis of FFPE tissue samples. After lysis DNA is directly bisulfite conversion without prior purification of DNA thus eliminating loss of DNA due to DNA isolation.