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PyroMark Q96 MD Accessories and Reagents

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For optimal sample preparation and Pyrosequencing analysis using PyroMark Q96 MD

Reliable quantification of genetic or epigenetic DNA modifications
Solutions for quick and easy sample handling for successful results
Dedicated products for each platform and each step in the workflow
High-quality optimized reagents enable reliable Pyrosequencing results

The PyroMark Q96 MD uses proven Pyrosequencing technology for real-time, sequence-based detection and quantification for genetic analysis and epigenetic methylation studies. PyroMark Q96 MD Accessories and Reagents are high-quality reagents and products that simplify and streamline the Pyrosequencing workflow and enable successful results. PyroMark Q96 MD Accessories and Reagents are designed specifically for use with PyroMark Q96 MD instruments.

Please note, the following products will be discontinued as of January 31, 2021. We recommend the alternative product, PyroMark 48. For more information, please contact QIAGEN Technical Services.

PyroMark Q96 HS Plate (100) (cat. no. 979101)
PyroMark Q96 HS Q96 Reagent Tip (4) (cat. no. 979102)
PyroMark Q96 HS Capillary Tip (8) (cat. no. 979104)

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Cat No./ID: 9001528

PyroMark Q96 Vacuum Workstation (110 V)

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Vacuum workstation for preparing 96 samples in parallel from PCR product to single-stranded template

Cat No./ID: 9001529

PyroMark Q96 Vacuum Workstation (220 V)

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Vacuum workstation for preparing 96 samples in parallel from PCR product to single-stranded template

Cat No./ID: 9001740

PyroMark Q96 Vacuum Workstation (100 V)

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Vacuum workstation for preparing 96 samples in parallel from PCR product to single-stranded template

Cat No./ID: 9019071

PyroMark Q96 HS Sample Prep Thermoplate

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Holder for placement of PyroMark Q96 Plate on heating block during annealing step prior to PyroMark Q96 MD analysis

Cat No./ID: 9019074

PyroMark Q96 HS Tip Holder Box

HK$735.00

Box for dust-free and safe storage of tips in between runs. For use with PyroMark Q96 MD

Cat No./ID: 9019075

PyroMark Q96 HS Dispensing Tip Holder

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Reusable holder for tips, nucleotide dispensing tips, and reagent dispensing tips, for use with PyroMark Q96 MD

Cat No./ID: 9019076

PyroMark Q96 HS Capillary Tip Holder

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Reusable holder for tips, nucleotide dispensing tips, and reagent dispensing tips, for use with PyroMark Q96 MD

Cat No./ID: 972804

PyroMark Gold Q96 Reagents (5 x 96)

HK$6,200.00

For performing Pyrosequencing reactions on the PyroMark Q96 ID (5 x 96) and PyroMark Q96 MD (15 x 96)

Cat No./ID: 972807

PyroMark Gold Q96 Reagents (50 x 96)

HK$18,790.00

For performing Pyrosequencing reactions on the PyroMark Q96 MD

Cat No./ID: 972824

PyroMark Gold Q96 CDT Reagents (6 x 96)

HK$3,280.00

For performing Pyrosequencing reactions on the PyroMark Q96 MD in combination with the capillary dispensing tips (CDT)

Cat No./ID: 978703

PyroMark PCR Kit (200)

HK$3,230.00

For 200 reactions: 2x PyroMark PCR Master Mix (includes HotStarTaq DNA Polymerase and optimized PyroMark Reaction Buffer containing 3 mM MgCl₂ and dNTPs), 10x CoralLoad Concentrate, 5x Q-Solution, 25 mM MgCl₂, and RNase-Free Water.

Cat No./ID: 978705

PyroMark PCR Kit (800)

HK$11,850.00

For 800 reactions: 2x PyroMark PCR Master Mix (includes HotStarTaq DNA Polymerase and optimized PyroMark Reaction Buffer containing 3 mM MgCl₂ and dNTPs), 10x CoralLoad Concentrate, 5x Q-Solution, 25 mM MgCl₂, and RNase-Free Water

Cat No./ID: 978746

PyroMark CpG Assay (200)

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PCR and sequencing primers for Pyrosequencing analysis of gene-specific CpG methylation after DNA bisulfite conversion (200 reactions; tube format)

Cat No./ID: 978754

PyroMark CpG Assay 96 well (50)

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PCR and sequencing primers for Pyrosequencing analysis of gene-specific CpG methylation after DNA bisulfite conversion (50 reactions; plate format); minimum order of 24 assays per plate

Cat No./ID: 978756

PyroMark CpG Assay 96 well (200)

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PCR and sequencing primers for Pyrosequencing analysis of gene-specific CpG methylation after DNA bisulfite conversion (200 reactions; plate format); minimum order of 24 assays per plate

Cat No./ID: 978776

PyroMark Custom Assay (200)

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User-designed PCR and sequencing primers for Pyrosequencing analysis (200 reactions; tube format)

Cat No./ID: 978801

PyroMark OneStep RT-PCR Kit (50)

HK$2,350.00

For 50 reactions: QIAGEN OneStep RT-PCR Enzyme Mix, optimized QIAGEN OneStep RT-PCR Buffer, 10x CoralLoad Concentrate, 5x Q-Solution, dNTP Mix, and RNase-Free Water

Cat No./ID: 978803

PyroMark OneStep RT-PCR Kit (200)

HK$7,110.00

For 200 reactions: QIAGEN OneStep RT-PCR Enzyme Mix, optimized QIAGEN OneStep RT-PCR Buffer, 10x CoralLoad Concentrate, 5x Q-Solution, dNTP Mix, and RNase-Free Water

Cat No./ID: 979006

PyroMark Binding Buffer (200 ml)

HK$629.00

For binding of biotinylated PCR product to Sepharose beads

Cat No./ID: 979007

PyroMark Denaturation Sol. (500 ml)

HK$642.00

For denaturation of double-stranded PCR product into single-stranded DNA template

Cat No./ID: 979008

PyroMark Wash Buffer (conc., 200 ml)

HK$639.00

For use with the PyroMark Q24 Vacuum Workstation and PyroMark Q96 Vacuum Workstation to wash and neutralize the immobilized DNA

Cat No./ID: 979009

PyroMark Annealing Buffer

HK$629.00

For annealing of sequencing primer to DNA template

Cat No./ID: 979010

PyroMark Vacuum Prep Filter Probe (100)

HK$3,880.00

Reusable filter probes for PyroMark Vacuum Workstation Q96 and Q24

Cat No./ID: 979011

PyroMark Q96 Vacuum Prep Trough (5)

HK$544.00

Reusable plastic troughs for use with all PyroMark Q96 Vacuum Workstations

Cat No./ID: 979203

PyroMark Control Oligo

HK$5,490.00

For installation check of system

PyroMark Q96 MD Accessories and Reagents are intended for molecular biology applications. These products are not intended for the diagnosis, prevention, or treatment of a disease.

Product Details

Applications

PyroMark Q96 MD Accessories and Reagents enable Pyrosequencing analysis with the PyroMark Q96 MD instrument for applications such as:

HRM
Methylation analysis
Mutation analysis
SNP analysis

Product Resources

FAQs (38)

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	What concentration should be used for the sequencing primer in pyrosequencing? FAQ ID -2826	View
	Can I order the nucleotides from PyroMark Gold Reagents separately? FAQ ID -2827	View
	Which purity grade is recommended for pyrosequencing primers? FAQ ID -2832	View
	How many times can vacuum troughs be re-used with the PyroMark Vacuum Preparation Stations? FAQ ID -2848	View
	Where can I order the Streptavidin Sepharose beads for pyrosequencing? FAQ ID -2850	View
	How many nucleotides of a homopolymer can be resolved in pyrosequencing? FAQ ID -2871	View
	Can unused wells in a pyrosequencing plate be used in the next run? FAQ ID -2872	View
	What is the reason for signals ceasing in the middle of a pyrosequencing run? FAQ ID -2875	View
	How do I reduce background peaks in the pyrosequencing pyrogram? FAQ ID -2877	View
	How do I prevent a drifting baseline in my pyrosequencing pyrogram? FAQ ID -2878	View
	What is the reason for a high substrate peak in the pyrosequencing pyrogram? FAQ ID -2879	View
	What is the reason for split peaks appearing in between dispensations on my pyrosequencing pyrogram? FAQ ID -2881	View
	How accurate and reliable is PyroMark Q24 in mutation analysis? FAQ ID -2094	View
	Which kits can be used in combination with the PyroMark CpG Assays and PyroMark Custom Assays? FAQ ID -2822	View
	What kind of shaker should be used for the pyrosequencing binding step? FAQ ID -2837	View
	Which heating block is recommended for the pyrosequencing annealing step? FAQ ID -2838	View
	Can PyroMark Gold reagents be vortexed? FAQ ID -2844	View
	What is the concentration of PyroMark Control Oligo? FAQ ID -2846	View
	What should be the single peak height for the PyroMark Control Oligo on the different PyroMark instruments? FAQ ID -2852	View
	What is the use of the PyroMark Q24 Validation Oligo? FAQ ID -2855	View
	How many times can the cartridges for Pyromark Q24 or PyroMark Q96 ID instruments are reused? FAQ ID -2863	View
	What is the purpose of the unmethylated and unconverted control DNA of the EpiTect PCR Control DNA Set? FAQ ID -2007	View
	How do I set up a PyroMark CpG Assay? FAQ ID -2814	View
	How are the PyroMark CpG Assays shipped and stored? FAQ ID -2816	View
	How are the PyroMark CpG Assays reconstituted? FAQ ID -2817	View
	Does QIAGEN offer a design of Custom PyroMark CpG Assays? FAQ ID -2818	View
	What are the features of PyroMark CpG Assays e.g. in terms of design, validation? FAQ ID -2821	View
	Will the primer sequence for the PyroMark CpG Assay be provided? FAQ ID -2823	View
	In which format can PyroMark CpG Assays be ordered? FAQ ID -2824	View
	What is the recommended amplicon size for CpG assays? FAQ ID -2825	View
	Is the CpG software included in the PyroMark instruments to study methylation status? FAQ ID -2842	View
	What is included in a PyroMark Custom Assay? FAQ ID -2815	View
	Which end of the PCR primer for pyrosequencing should be biotinylated? FAQ ID -2839	View
	Will dUTP in a PCR reaction affect pyrosequencing? FAQ ID -2843	View
	Which PyroMark Gold Q96 Reagent should be used for which instrument and application? FAQ ID -2836	View
	What is the meaning of the abbreviations NDT and CDT and what are the differences? FAQ ID -2841	View
	How many times can the CDTs, NDTs, and RDTs be used? FAQ ID -2865	View
	Does the Pyromark Assay Design or application software give any cycling conditions for individual assay primers or a PCR setup pipetting scheme? FAQ ID -2862	View

Brochures & Guides (2)

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	Product Profile - PyroMark Q24 Advanced	Show details
	Pyrosequencing – the synergy of sequencing and quantification	Show details

Kit Handbooks (7)

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	PyroMark Control Oligo Handbook For use with PyroMark Q48 Autoprep, PyroMark Q24 Advanced, PyroMark Q24, PyroMark Q96 ID and PyroMark Q96 MD systems	Show details
	(EN) - PyroMark Gold Q96 Reagents Handbook For performing Pyrosequencing reactions on the PyroMark Q96 ID, PyroMark Q96 MD, and PyroMark Q96 MD Automated	Show details
	(EN) - PyroMark OneStep RT-PCR Handbook	Show details
	(EN) - PyroMark PCR Handbook	Show details
	(EN) - PyroMark Q96 Capillary Tip Product Sheet	Show details
	(EN) - PyroMark Q96 Nucleotide Tip Product Sheet	Show details
	(EN) - PyroMark Q96 Reagent Tip Product Sheet	Show details

Additional Resources (2)

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	(EN) - Offline Ordering Instructions for PyroMark CpG Assays	Show details
	(EN) - Offline Ordering Instructions for PyroMark Custom Assays	Show details

Scientific Posters (2)

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	(EN) - PyroMark CpG Assays: A new tool for genome-wide methylation profiling by Pyrosequencing	Show details
	Advanced DNA methylation analysis for verification of Infinium HumanMethylation450 BeadChip data Scientific poster	Show details

User-Developed Protocols (1)

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	Pyrosequencing® method for the forensic identification of tissue source using QIAGEN’s PyroMark® Q24 Advanced system - (EN)	Show details

White Papers (1)

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	(EN) - Techniques to overcome bottlenecks in epigenetics research	Show details

Application Notes (2)

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	Tissue source attribution using the PyroMark Q48 Autoprep System: Sperm identification in forensic casework	Show details
	Pyrosequencing – tissue source forensic identification using PyroMark Q24 Advanced A Pyrosequencing method for the forensic identification of tissue source using QIAGEN’s PyroMark Q24 Advanced system.	Show details

Quick-Start Protocols (2)

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	(EN) - PyroMark PCR Kit Quick-Start Protocol	Show details
	PyroMark Q96 Vacuum Workstation Quick-Start Guide - (EN)	Show details

Safety Data Sheets (18)

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	MSDS PyroMark Annealing Buffer
	MSDS PyroMark Binding Buffer (200 ml)
	MSDS PyroMark Control Oligo
	MSDS PyroMark CpG Assay (200)
	MSDS PyroMark CpG Assay 96 plate (200)
	MSDS PyroMark CpG Assay 96 plate (50)
	MSDS PyroMark Custom Assay (200)
	MSDS PyroMark Denaturation Sol. (500 ml)
	MSDS PyroMark Gold Q96 CDT Reagents (6 x 96)
	MSDS PyroMark Gold Q96 Reagents (5 x 96)
	MSDS PyroMark Gold Q96 Reagents (50 x 96)
	MSDS PyroMark OneStep RT-PCR Kit (200)
	MSDS PyroMark OneStep RT-PCR Kit (50)
	MSDS PyroMark PCR Kit (200)
	MSDS PyroMark PCR Kit (800)
	MSDS PyroMark Vacuum Prep Filter Probe (100)
	MSDS PyroMark Wash Buffer (conc., 200 ml)
	CofA

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PyroMark Gold Q24 Reagents (5 x 24)

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PyroMark Q48 Magnetic Beads (300)

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PyroMark Q24 Plate (100)

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PyroMark Q48 Discs (50)

50 discs for automated template preparation and Pyrosequencing reactions using a PyroMark Q48 Autoprep

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PyroMark Q48 Advanced Reagents (4 x 48)

Reagents for 4 x 48 PyroMark Q48 Autoprep standard reactions: PyroMark Advanced Enzyme Mix, PyroMark Advanced Substrate Mix, Denaturation Solution, Annealing Buffer, Binding Buffer, Nucleotides

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15 ml digestion buffer for use with the QIAsymphony RNA Kit (FFPE protocol), RNeasy FFPE Kit, Allprep FFPE DNA/RNA Kit or miRNeasy FFPE Kit

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