MaXtract High Density

Para una extracción de ácidos nucleicos más segura y práctica a partir de disolventes orgánicos

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MaXtract High Density (200 x 2 ml)

Cat. No. / ID:  129056

MaXtract High Density Tubes (200 x 2 ml)
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294,00 US$
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Quantity
200 x 2 ml
200 x 1.5 ml
100 x 15 ml
25 x 50 ml
This product will be discontinued as of December 31, 2024 or until stocks last.
El MaXtract High Density está concebido para su uso en aplicaciones de biología molecular. Este producto no está concebido para el diagnóstico, la prevención ni el tratamiento de enfermedades.

✓ 24/7 automatic processing of online orders

✓ Knowledgeable and professional Product & Technical Support

✓ Fast and reliable (re)-ordering

Features

  • Extracción de ácidos nucleicos más segura a partir de disolventes orgánicos
  • Reducción del arrastre de contaminantes
  • Alta recuperación de ácidos nucleicos
  • Recuperación fácil de ácidos nucleicos

Product Details

MaXtract High Density simplifica la extracción de ácidos nucleicos a partir de disolventes orgánicos (por ejemplo, fenol/cloroformo). El gel MaXtract forma una barrera estable entre el disolvente orgánico y la fase acuosa que contiene los ácidos nucleicos, lo que permite la recuperación de la fase acuosa y previene el arrastre de soluciones orgánicas, proteínas y otros contaminantes.

Performance

El MaXtract High Density permite una recuperación un 30 % mayor en comparación con los métodos tradicionales de extracción. Las proteínas y otros contaminantes migran hacia la fase orgánica y a la interfase. El gel MaXtract High Density forma una barrera lo suficientemente duradera como para que estos contaminantes queden atrapados por debajo de la barrera, lo que evita el arrastre al eliminar la fase acuosa. La fase acuosa puede eliminarse mediante decantación o pipeteo, lo que da lugar a una recuperación de hasta un 30 % más de ácidos nucleicos que los métodos tradicionales de extracción orgánica.

MaXtract High Density se suministra en varios tamaños de tubo, lo que permite la extracción de volúmenes de muestra que van desde 100 µl a 20 ml.

Separación de fases con MaXtract High Density
Fase acuosaFenol:cloroformoCloroformo
NaCI <0,5 M, <1 mg/ml de proteína
NaCl ≥0,5 M
≥1 mg/ml de proteína
Aislamiento del ADN plasmídico
Aislamiento del ADN genómico
Aislamiento de ARN

Principle

El gel MaXtract High Density proporciona una recuperación rápida y segura de ácidos nucleicos a partir de extracciones orgánicas con el uso de disolventes como el fenol/cloroformo (consulte el diagrama de flujo “ Procedimiento MaXtract”).
See figures

Procedure

Simplemente añada la solución de ácido nucleico y el disolvente orgánico al tubo que contiene el gel MaXtract. Tras mezclar y centrifugar, el gel MaXtract High Density forma una barrera estable que separa las fases orgánica y acuosa. La barrera atrapa de forma segura el disolvente orgánico peligroso y los contaminantes, lo que permite decantar o pipetear de forma fácil la fase acuosa que contiene ácidos nucleicos en un tubo nuevo (consulte la figura “ Extracción segura y fácil de ácidos nucleicos”). Si se necesita una segunda extracción, se puede realizar en el mismo tubo, siempre y cuando no se supere el volumen máximo del tubo.

La separación con el gel MaXtract High Density está basada en las diferencias de densidad entre los medios acuoso y orgánico. La densidad de la capa orgánica debe ser mayor que la del gel y la fase acuosa, y la densidad del gel debe ser mayor que la de la fase acuosa.

See figures

Applications

MaXtract High Density es especialmente adecuado para el aislamiento de ADN plasmídico, ADN genómico o ARN. También existen protocolos optimizados para aplicaciones específicas, como la recuperación de ADN a partir de agarosa de bajo punto de fusión. Los ácidos nucleicos extraídos con el gel MaXtract High Density son idóneos para muchas aplicaciones posteriores.

Supporting data and figures

FAQ

Can phenol/chloroform be placed in the MaXtract tube for extraction at a later time?

Yes. The organic phase can be placed in MaXtract Low and High Density tubes without any problem 3-4 hours before preparation of the samples. Make sure to perform the centrifugation step first, so that the gel collects at the bottom of the tube.

 

FAQ ID -1299
Can MaXtract tubes be autoclaved prior to use?

No. After autoclaving, the MaXtract gel no longer has the same properties. Since the MaXtract tubes are manufactured and filled in a fully automated process, autoclaving is not required.

 

 

FAQ ID -1305
Can DNA isolated with MaXtract tubes be used for downstream applications such as restriction digestion, labeling, blotting, PCR, and automated sequencing?

DNA purified by organic extraction can principally be used for these downstream reactions. It should be noted that phenol, like all organic solvents, alters the active centre of enzymes, thus deactivating them. An ethanol precipitation should therefore generally be carried out prior to enzyme controlled downstream reactions. Potential phenol residue is thereby effectively removed.

The use of MaXtract Low and High Density tubes has the advantage that no recontamination of the sample by organic solvents or proteins occurs.

 

FAQ ID -1303
What factors determine if denatured proteins accumulate underneath, or inside the gel of the MaXtract Low and High Density Tubes?

Generally, this is determined by the density of the denatured proteins and the gel type in the MaXtract Tubes. If proteins show a higher density than the gel, they will accumulate underneath in the organic phase. If they have the same density as the MaXtract gel, they will collect in the gel. The quantity and ratio of phenol:chloroform can also influence the behavior of the proteins.

 

FAQ ID -1312
Do you have a protocol for purification of total RNA from fatty tissues using QIAzol Lysis Reagent and MaXtract High Density?

Yes, we have the following protocols:

  • Purification of total RNA from fatty tissues using QIAzol Lysis Reagent, MaXtract High Density, and the TissueRuptor (RY29).
  • Purification of total RNA from fatty tissues using QIAzol Lysis Reagent, MaXtract High Density, and the TissueLyser (RY30).
  • Purification of total RNA from fatty tissues using the RNeasy Lipid Tissue Mini Kit and MaXtract High Density (RY31).
FAQ ID -1550
Is it possible to use MaXtract for an organic extraction with a mixture of phenol and BCP (1 bromine chlorpropane)?

Yes, this application is possible with both MaXtract Low and High Density Tubes. A sufficient quantity of BCP should be used when using MaXtract High Density, in order to achieve a stable gel phase. BCP increases the density of the organic phase in the same way as chloroform.

 

-3
Does the addition of sample and organic solution to the MaXtract Tubes require a specific pipetting sequence?

No. The functional efficiency of the MaXtract Low and High Density Tubes does not depend on the pipetting sequence.

 

FAQ ID -1307
Can frozen sample material pulverized in liquid nitrogen be directly added to MaXtract tubes?

If organic solvents are added immediately following the filling of MaXtract Low and High Density Tubes with the frozen sample, it should not have any negative effect on the functionality of the tubes.

 

FAQ ID -1301
Can the MaXtract High Density Tube be used with QIAzol to isolate RNA?
FAQ ID -1304
Which density gel type is contained in the yellow or green MaXtract Tubes?

MaXtract High Density gel is found in the yellow tubes, and MaXtract Low Density gel is contained in the green tubes.

 

FAQ ID -1315
Can several extraction steps be performed in the same MaXtract tube?

Yes, if a sufficiently large MaXtract tube has been selected for the extraction it is possible to perform multiple purification steps in this tube. Sample and organic solvent are placed in the MaXtract tube, mixed and centrifuged. Organic solvent is then added again to the aqueous phase separated by the gel, followed by mixing and centrifuging. Following the last extraction step, the sample is transferred to a new tube for storage.

For the purification of samples with high protein content, we recommend using a new MaXtract tube for each extraction step.

 

FAQ ID -1302
When isolating DNA from plant cells using CTAB, should MaXtract Low or High Density be used?

CTAB as a rule increases the density of the aqueous phase. Therefore, MaXtract High Density should be used for the organic extraction of DNA from CTAB samples.

Depending on the amount of CTAB used, the density of the sample can become too high for the MaXtract High Density Tube, leading to a gel barrier above the aqueous phase. In this case, puncture the gel with the tip of a pipette and dilute the aqueous phase with either distilled water or TE buffer. Mix again and then centrifuge. However, we recommend to repeat the separation in a new MaXtract tube.

 

FAQ ID -1308
Can a phenol-chloroform ratio different from 1:1 also be used with MaXtract Low and High Density?

A phenol-chloroform ratio of 1:1 is advantageous for the phase separation when using MaXtract Low and High Density, since the MaXtract interphase has the greatest stability at this ratio.

Methods using a phenol-chloroform ratio of as much as 6:1 are also possible with MaXtract. However, MaXtract can partly precipitate onto the bottom of the tube in this case.

If the phenol-chloroform ratio is greater than 1:1, it is necessary to use MaXtract Low Density Tubes. Note that this MaXtract type is not compatible with applications in which the aqueous phase has a high density (e.g., isolation of plasmid DNA and RNA).

 

FAQ ID -1309
If both MaXtract Low Density and High Density is suitable for an application, which type is preferable to use?

In this case, both MaXtract Low and High Density Tubes can be used with the same success. We principally recommend testing both MaXtract types.

 

 

FAQ ID -1310
Are MaXtract tubes siliconized?

No, MaXtract Low and High Density tubes are not coated in any way.

 

FAQ ID -1298