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QIAGEN Plasmid Kits

For purification of up to 10 mg transfection-grade plasmid or cosmid DNA

  • Purity equivalent to 2 x CsCl gradient centrifugation
  • High yields of plasmid DNA
  • Cost-effective preparations
  • LyseBlue for optimum lysis and maximum DNA yield

QIAGEN Plasmid Kits provide gravity-flow, anion-exchange tips for purification of transfection-grade plasmid DNA. Lysate clearing and isopropanol precipitation are achieved by centrifugation.

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Cat No./ID: 10023
QIAGEN-tip 20 (25)
25 columns
Cat No./ID: 10043
QIAGEN-tip 100 (25)
25 columns
Cat No./ID: 10063
QIAGEN-tip 500 (25)
25 columns
Cat No./ID: 10083
QIAGEN-tip 2500 (25)
25 columns
Cat No./ID: 10091
QIAGEN-tip 10000 (5)
5 columns
Cat No./ID: 12123
QIAGEN Plasmid Mini Kit (25)
25 QIAGEN-tip 20, Reagents, Buffers
Cat No./ID: 12125
QIAGEN Plasmid Mini Kit (100)
100 QIAGEN-tip 20, Reagents, Buffers
Cat No./ID: 12143
QIAGEN Plasmid Midi Kit (25)
25 QIAGEN-tip 100, Reagents, Buffers
Cat No./ID: 12145
QIAGEN Plasmid Midi Kit (100)
100 QIAGEN-tip 100, Reagents, Buffers
Cat No./ID: 12145X4
QIAGEN Plasmid Midi Kit (400)
400 QIAGEN-tip 100, Reagents, Buffers
Cat No./ID: 12162
QIAGEN Plasmid Maxi Kit (10)
10 QIAGEN-tip 500, Reagents, Buffers
Cat No./ID: 12163
QIAGEN Plasmid Maxi Kit (25)
25 QIAGEN-tip 500, Reagents, Buffers
Cat No./ID: 12165
QIAGEN Plasmid Maxi Kit (100)
100 QIAGEN-tip 500, Reagents, Buffers
Cat No./ID: 12165X4
QIAGEN Plasmid Maxi Kit (400)
400 QIAGEN-tip 100, Reagents, Buffers
Cat No./ID: 12181
QIAGEN Plasmid Mega Kit (5)
5 QIAGEN-tip 2500, Reagents, Buffers
Cat No./ID: 12183
QIAGEN Plasmid Mega Kit (25)
25 QIAGEN-tip 2500, Reagents, Buffers
Cat No./ID: 12191
QIAGEN Plasmid Giga Kit (5)
5 QIAGEN-tip 10000, Reagents, Buffers
Cat No./ID: 19046
Plasmid Buffer Set
Buffers P1, P2, P3, QBT, QC, QF, RNase A; for 100 plasmid mini-, 25 midi-, or 10 maxipreps
QIAGEN Plasmid Kits are intended for molecular biology applications. These products are not intended for the diagnosis, prevention, or treatment of a disease.

Product Details

9
QIAGEN Plasmid Kit procedures.
Neutralized bacterial lysates are cleared by centrifugation. The cleared lysate is then loaded onto the anion-exchange tip where plasmid DNA selectively binds under appropriate low-salt and pH conditions. RNA, proteins, metabolites, and other low-molecular-weight impurities are removed by a medium-salt wash, and ultrapure plasmid DNA is eluted in high-salt buffer. The DNA is concentrated and desalted by isopropanol precipitation and collected by centrifugation.
..
9
Transfection efficiency versus plasmid purification method.
Different pRSVcat DNA preparations using the methods indicated were introduced into the indicated cell lines by liposome-mediated transfection, and the efficiencies determined by measuring CAT expression levels after 40 h. Each bar represents the mean of 4 independent transfections (2 transfections with each of 2 independent plasmid preparations). The highest transfection efficiency was achieved with QIAGEN Plasmid Kits.
Performance
The QIAGEN Plasmid Kits uses gravity-flow QIAGEN anion-exchange tips for efficient purification of plasmid DNA. Up to
10 mg (giga), 2.5 mg (mega), 500 µg (maxi), 100 µg (midi), and 20 µg (mini) high-copy plasmid DNA is purified from culture (culture volumes depend on plasmid copy number, size of insert, host strain, and culture medium). Plasmid DNA purified with QIAGEN Plasmid Kits is highly suitable for use in applications such as transfection (see figure "Transfection efficiency versus plasmid purification method"), cloning, and in vitro transcription.
Principle

The unique anion-exchange resin in QIAGEN-tips is developed exclusively for the purification of nucleic acids. Its exceptional separation properties result in DNA purity equivalent or superior to that obtained by two successive rounds of CsCl gradient centrifugation. Prepacked QIAGEN-tips operate by gravity flow and never run dry, minimizing the hands-on time required for plasmid preparation. The entire QIAGEN plasmid purification system avoids the use of toxic substances such as phenol, chloroform, ethidium bromide, and CsCl, minimizing hazard both to the user and the environment.

Specifications

Features
Plasmid
Giga Kit
Plasmid
Mega Kit
Plasmid
Maxi Kit
Plasmid
Midi Kit
Plasmid
Mini Kit
Applications Transfection, cloning, sequencing, capillary sequencing, etc. Transfection, cloning, sequencing, capillary sequencing, etc. Transfection, cloning, sequencing, capillary sequencing, etc. Transfection, cloning, sequencing, capillary sequencing, etc. Transfection, cloning, sequencing, capillary sequencing, etc.
Culture volume/starting material 2.5–5 liters culture volume 500 ml – 2.5 liters culture volume 100–500 ml culture volume 25–100 ml culture volume 3–10 ml culture volume
Elution volume Variable Variable Variable Variable Variable
Plasmid type High-copy, low-copy, cosmid DNA High-copy, low-copy, cosmid DNA High-copy, low-copy, cosmid DNA High-copy, low-copy, cosmid DNA High-copy, low-copy, cosmid DNA
Processing Manual (centrifugation) Manual (centrifugation) Manual (centrifugation) Manual (centrifugation) Manual (centrifugation)
Sample per run 1 sample per run 1 sample per run 1 sample per run 1 sample per run 1 sample per run
Technology Anion-exchange technology Anion-exchange technology Anion-exchange technology Anion-exchange technology Anion-exchange technology
Time per run 320 min 220 min  160 min 150 min 80 min
Yield <10 mg <2.5 mg <500 µg up to 100 µg <20 µg
 

 

Procedure

With QIAGEN Plasmid Kits, bacterial lysates are cleared by centrifugation. The cleared lysate is then loaded onto the anion-exchange tip where plasmid DNA selectively binds under appropriate low-salt and pH conditions. RNA, proteins, metabolites, and other low-molecular-weight impurities are removed by a medium-salt wash, and pure plasmid DNA is eluted in high-salt buffer (see flowchart "QIAGEN Plasmid Kit procedures"). The DNA is concentrated and desalted by isopropanol precipitation and collected by centrifugation. 

Applications

Plasmid DNA purified with QIAGEN Plasmid Kits is highly suitable for use in applications, such as:

  • Transfection
  • Cloning
  • PCR
  • In vitro transcription 

Specifications

Features
Specifications
Applications Transfection, cloning, sequencing, capillary sequencing etc.
Culture volume/starting material 2.5–5 liters culture volume
Plasmid type High-copy, low-copy, cosmid DNA
Processing Manual (centrifugation)
Samples per run (throughput) 1 sample per run
Technology Anion-exchange technology
Time per run or prep per run 320 min
Yield <10 mg

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