QIAwave RNA Plus Mini Kit

More eco-friendly alternative to our RNeasy Plus Mini Kit for fast purification total RNA from cells and tissues and integrated gDNA removal

Want to try this solution for the first time?
Get in touch with our team today and request a quote for your QIAwave RNA Plus Mini Kit (50) trial kit.
QIAwave RNA Plus Mini Kit (50)

Cat. No. / ID:  74634

For 50 minipreps: RNeasy Mini Spin Columns, gDNA Eliminator Spin Columns, Waste Tubes, RNase-Free Water and Buffers
CHF 545.00
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For
50
250
The QIAwave RNA Plus Mini Kit (50) is intended for molecular biology applications. This product is not intended for the diagnosis, prevention, or treatment of a disease.
Want to try this solution for the first time?
Get in touch with our team today and request a quote for your QIAwave RNA Plus Mini Kit (50) trial kit.

Features

  • Phenol-free high-quality total RNA isolation in minutes using fast and simple extraction protocols
  • RNA quality, yields and performance identical to the RNeasy Plus Mini Kit
  • Efficient gDNA removal with unique gDNA Eliminator columns (no need for DNase)
  • Up to 47% less plastic and up to 42% less cardboard compared to the RNeasy Plus Mini Kits
  • Reusable Waste Tubes made from 100% post-consumer recycled plastic
  • Buffer concentrates that use up to 90% less plastic than our standard buffer bottles

Product Details

The QIAwave RNA Plus Mini Kit is a more eco-friendly version of our standard RNeasy Plus Mini Kit, combining high-quality RNA purification with effective gDNA removal using unique gDNA Eliminator columns. Total RNA of high purity and yield is obtained from a wide range of cultured cells and easy-to-lyse tissues. 


The QIAwave RNA Plus Mini Kit uses up to 47% less plastic and up to 42% less cardboard than our standard kit and offers Waste Tubes made from 100% post-consumer recycled plastic that you can reuse throughout the procedure. QIAwave buffers also come as concentrates, reducing the amount of plastic by up to 90% per bottle. To save paper, there are no printed protocols in the kit. Instead, you can download the protocols you need from the resources list or by simply scanning the QR code inside the box lid. So, while the kit packaging and components of our QIAwave RNA Plus Mini Kit may look different, it’s as easy to use as the RNeasy Plus Mini Kit, and the chemistry and performance are identical.


Please be aware that you will need sterile glass bottles to store the reconstituted buffers.

In partnership with My Green Lab, we've assessed the environmental impact of this kit. My Green Lab ACT labels evaluate and score products on several sustainability criteria:

  • Manufacturing
  • Responsible chemical management
  • Sustainable content within products and packaging materials
  • Disposal of the packaging at the end of life

Products are scored from 1 to 10 except for energy and water consumption, which are scored 1 point per kWh or gallon, respectively. A low score means a lower impact (see figures "QIAwave RNA Plus Mini Kit ACT label US  50/ 250, EU  50/ 250 and UK  50/ 250").

 

See figures

Performance

The QIAwave RNA Plus Mini Kit gives you highly reproducible yields of total RNA from cells and tissues.  The performance between our QIAwave RNA Plus Mini Kit and the RNeasy Plus Mini Kit is identical because the chemistry is the same. Further, the kit performs similarly or better compared to a competitor kit (see figure “ RNA yields obtained with QIAwave RNA Plus Mini Kit”). Total RNA isolated with the QIAwave RNA Plus Mini Kit results in similar downstream RT-qPCR performance as total RNA isolated with the RNeasy Plus Mini Kit, as well as similar or better performance compared to a competitor kit. (see figure: “ RT-qPCR Performance of total RNA isolated with QIAwave RNA Plus Mini Kit”) 

We have also compared RNA yields obtained with the QIAwave RNA Plus Mini Kit (50) preparing the buffer either by pouring or pipetting and with the RNeasy Plus Mini Kit (50) using standard buffers. All methods result in comparable RNA yields as shown in the figure “ Handling of buffer concentrates”.

See figures

Principle

The QIAwave RNA Plus Mini procedure integrates QIAGEN’s innovative technology for selective binding of double-stranded DNA with well-established RNeasy technology. Efficient purification of high-quality RNA is guaranteed, without the need for additional DNase digestion. The purified RNA is ready to use and is ideally suited for downstream applications that are sensitive to low amounts of DNA contamination, such as quantitative, real-time RT-PCR and dPCR.


Cells and easy-to-lyse tissues are first lysed and homogenized in highly denaturing guanidine-isothiocyanate–containing Buffer RLT Plus, which immediately inactivates RNases to ensure isolation of intact RNA. The lysate is then passed through a gDNA Eliminator spin column that, in combination with the high-salt buffer, selectively and efficiently removes genomic DNA. Ethanol is added to provide appropriate binding conditions for RNA, and the sample is applied to an RNeasy spin column. These specialized columns contain a silica membrane that specifically binds RNA from lysed cells.

 

Procedure

The QIAwave RNA Plus Mini Kit isolates total RNA from up to 107 cells or 30 mg tissue. A short workflow enables RNA isolation with genomic DNA removal in less than 25 minutes (see flowchart  “ QIAwave RNA Plus Mini procedure”). Samples are first lysed and homogenized. The lysate is passed through a gDNA Eliminator spin column, ethanol is added to the flow-through, and the sample is applied to an RNeasy spin column. RNA binds to the membrane and contaminants are washed away. High-quality RNA is eluted in 30 µL water.

Different protocols are available for different starting materials. The protocols differ mainly in the lysis and homogenization of the sample. Once the sample is applied to the gDNA Eliminator spin column, the protocols are similar. The procedure provides an enrichment for mRNA since most RNAs <200 nucleotides (such as rRNAs and tRNAs) are excluded.


When disrupting and homogenizing tissues in Buffer RLT Plus (supplied with the RNeasy Plus Mini Kit), excessive foaming may occur. This foaming is substantially reduced by adding Reagent DX (supplied separately) to Buffer RLT Plus before starting disruption and homogenization. Reagent DX has been carefully tested with the kit, and has no effect on RNA purity or on downstream applications.


The QIAwave RNA Plus Mini Kit can be automated on the QIAcube Connect using the RNeasy Plus Mini Kit protocol. 

See figures

Applications

RNA purified using the QIAwave RNA  Plus Mini Kit is ideally suited for downstream applications that are sensitive to low amounts of DNA contamination, such as RNA-seq and quantitative real-time RT-PCR. The purified RNA can also be used in other applications.

Supporting data and figures

Specifications

FeaturesSpecifications
ApplicationsRNA-seq, quantitative real-time RT-PCR, end-point RT-PCR, Northern dot and slot blotting, microarray analysis
Elution volume30-50 µL
TechnologySilica technology
FormatSpin column
Integrated removal of genomic DNAYes
Sample typeCells, tissues
Sample amount107 cells or 30 mg tissue
ProcessingManual or automated on QIAcube Connect
Purification of total RNA, miRNA, poly A+ mRNA, DNA or proteinTotal RNA
Time per run25 minutes

Resources

Application/Protocol Documents (1)
Step up your sustainability by recycling your labware. This handy guide will show you how to quickly and easily recycle kit components and reduce plastic waste in your lab.
Quick-Start Protocols (1)
Brochures & Guides (2)
Kit Handbooks (1)
Safety Data Sheets (1)
Certificates of Analysis (1)

FAQ

I don’t have any glassware in the lab, is the QIAwave kit still a good option for me?
The concept of QIAwave includes the reconstitution of functional buffers. We recommend the use of glass bottles for this procedure. Glass bottles are easier to clean, sterilize and reuse than plastic bottles, further reducing the plastic footprint of the kit. If you do not have the option of using clean glass or plastic bottles, we recommend using our legacy kits. 
FAQ ID - 3989

Can the QIAwave kits be recycled?
We provided an infographic describing the composition of most of our purification kits. You can use the information provided as a guide to recycle kit components and use it to reduce plastic waste in your laboratory. Depending on the specific kit and application, certain kit components may contain or come into contact with chemicals and biological samples, in this case, the components should be disposed of according to local guidelines and regulations. You can find more information on More Sustainable Products (qiagen.com).
FAQ ID - 3992
What water should I use to prepare the buffer concentrates?
We recommend using highly pure water for reconstitution. Ultrapure water (such as the one from MilliQ system, also known as type 1 water) with a resistivity of 18.2 MΩ-cm at 25°C can be used. In case you do not have access to type 1 water, QIAGEN offers Nuclease-Free Water (5 L, cat. No. 129117); and Nuclease-Free Water (1000 mL, cat. No. 129115). It is important that you do not use tap water as this may interfere with the extraction of the target analyte. 
FAQ ID - 3986

Is there a way to compare the environmental impacts of the kit?
In partnership with My Green Lab, we were able to assess the environmental impact of the kits. My Green Lab ACT (accountability, consistency and transparency) environmental impact factor labels are designed to evaluate and score products on several sustainability criteria. The products are scored from 1 to 10, except for energy and water consumption which are scored as 1 point per kWh or gallon, respectively. A low score means a lower environmental factor.  
FAQ ID - 3991

Does the reuse of the Waste Tubes increase the risk of cross-contamination?
No, we were able to prove experimentally that cross-contamination does not occur through the reuse of the Waste Tubes. The Waste Tubes are used to collect the flow-through from the lysis and washing steps, where the flow-through is discarded afterwards. If the flow-through is to be used for further processing, we recommend the use of a Collection Tube
FAQ ID - 3988

Are the QIAwave kits based on a different chemistry than the legacy kits?
No, the chemistry between the QIAwave kits and the legacy kits is the same, and so is the performance. The QIAwave buffers come as concentrates, reducing the amount of plastic per bottle while maintaining the same functionality after reconstitution. The advantage of the QIAwave kits is the reduction of materials used for the kits, such as plastics, cardboard and paper. In addition, the QIAwave kits contain Waste Tubes made from 100% post-consumer plastic. 
FAQ ID - 3990

What is the new Waste Tube made of?
The new Waste Tube is made from recycled plastic recovered from post-consumer plastic waste. Due to the slight composition differences of the raw material, the color of the tubes may differ from lot to lot. However, this has no effect on its intended use of collecting flow-through from sample binding and membrane washing. After each step, the flow-through is discarded, and the Waste Tube can be reused. The Waste Tube is only used to process waste and should never come into direct contact with the analyte of interest. For detailed instructions, you can watch our instructional video at www.qiagen.com/qiawavewastetube
FAQ ID - 3987