QIAxcel RNA QC Kit v2.0

使用QIAxcel系统进行自动化定性和定量RNA分析

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QIAxcel RNA High Sensitivity Kit (1200)

Cat. No. / ID: 929112

QIAxcel RNA High Sensitivity Gel Cartridge, Buffers, Mineral Oil, QX Intensity Calibration Marker, QX RNA Alignment Marker, QX RNA Size Marker 200–6000 nt, QX RNA Denaturation Buffer, 12-Tube Strips

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Cartridge type

High Sensitivity

RNA QC

Note: The QIAxcel High Sensitivity Kit only works with the QIAxcel Connect

QIAxcel RNA QC Kit v2.0 旨在用于分子生物学应用。这些产品不能用于疾病诊断、预防和治疗。

✓ 24/7 automatic processing of online orders

✓ Knowledgeable and professional Product & Technical Support

✓ Fast and reliable (re)-ordering

Features

采用安全、操作简单的即用型预制胶
简化的工作流程，分析时间最短化
可检测到低至5 ng/μl的总RNA，结果可靠
比琼脂糖凝胶电泳更高的灵敏度
标准化的处理过程，包括数字化数据采集

Product Details

配合QIAxcel体系，QIAxcel RNA QC Kit v2.0每批可全自动定量和定性分析多达96个RNA样本。全自动上样和分析减少了对样本的手工操作，将RNA降解和污染的风险降到最低。该体系可检测低至5 ng/μl的稀释后总RNA（原始浓度10 ng/µl）和50 ng/µl的cRNA或单链cDNA。

Performance

快速、灵敏的分析RNA

在基因表达分析和miRNA研究中，RNA的完整性是获得可靠结果的一个关键因素，用于一些敏感的技术如定量PCR和微芯片分析。由于它们会强烈影响检测方法的预测能力，监测初始总RNA样本以及整个流程的产物的完整性是至关重要的。全自动QIAxcel仪能够快速、灵敏的分析总RNA和片段或完整的cRNA的完整性以及对它们进行定量。QIAxcel仪可方便的分析样本的降解水平以及完整性（参见" Analysis of degraded RNA"）。全自动上样和分析减少了对样本的手工操作，将RNA降解和污染的风险降到最低（参见" Analysis of plant RNA"）。

在QIAxcel仪上进行RNA分析高度可靠，并获得与Agilent Bioanalyzer相当的结果（见表）。

高度可靠的结果
75°C条件下的时间（分钟）	QIAxcel 1 28S/18S比值	QIAxcel 2 28S/18S比值	Agilent Bioanalyzer 28S/18S比值
0	1.59	1.53	1.7
2	1.42	1.55	1.6
4	1.31	1.45	1.6
7	1.35	1.36	1.5
13	1.16	1.18	1.2
20	1.09	1.07	1.3
30	0.68	0.72	1.1
40	0.58	0.53	0.8
52	0.64	0.61	0.5
70	0.34	0.38	0.3

See figures

Analysis of degraded RNA.

Analysis of plant RNA.

Principle

QIAxcel体系采用毛细管凝胶电泳技术，能够依据核酸的大小进行快速分离（参见""）。不同于传统的琼脂糖凝胶电泳，分离是在含有即用型预制胶的毛细管中进行。样本自动上样到单个的毛细管中，然后加压。带负电的核酸通过毛细管移到正极。使用琼脂糖凝胶电泳时，小分子核酸比大分子核酸移动得快。待测分子在毛细管中迁移，它们会通过一个检测和衡量荧光信号的检测器。光电倍增管将荧光信号转为电子数据，然后传输到电脑上再使用QIAxcel ScreenGel软件进一步处理。处理后，可通过电泳图谱和胶图两种方式查看数据。

See figures

Sample separation process.

Procedure

QIAxcel RNA QC Kit v2.0简化了分离和分析RNA的流程，且更加安全。只需简单的几个步骤即可：装载预制胶卡夹、预装缓冲液槽及装载有样本的96孔板或PCR试管或联管、选择分离方法。无需冗长的制胶及用户培训，简化了日常工作的整个流程。运行开始几分钟内，会在电脑屏幕上实时出现第一个结果。

Applications

QIAxcel RNA QC Kit v2.0能够快速、灵敏的分析总RNA和cRNA（片段或完整的）的完整性以及对它们进行定量检测。

Supporting data and figures

Sample separation process.

Nucleic acid molecules are separated according to size in gel-filled capillaries.

Streamlined RNA analysis using the QIAxcel system.

Resources

A protocol for evaluating quality of RNA samples using the QIAxcel Advanced Instrument and ScreenGel software version 1.3 or higher

Download Safety Data Sheets for QIAGEN product components.

For automated quantitative and qualitative RNA analysis using the QIAxcel and QIAxcel Advanced instruments

For automated quantitative and qualitative RNA analysis using the QIAxcel Connect instrument

For automated quantitative and qualitative RNA analysis using the QIAxcel and QIAxcel Advanced instruments

For use with QIAxcel Advanced instruments and QIAxcel ScreenGel Software version 1.6

FAQ

The intensity calibration procedure on the QIAxcel System and QIAxcel Advanced is performed to normalize the signal intensities across all 12 channels of the gel cartridge. This corrects for natural intensity reading variations between each capillary in the cartridge. A correction factor is determined and applied for every subsequent run performed with the cartridge.

FAQ ID -1824

No, the RNA gel itself is not denaturing. It is a native polymer gel.

FAQ ID - 3366

When analysing RNA Integrity Score with the QIAxcel RNA High Sensitivity Kit - samples must be in the concentration range of 1 ng/µL - 50 ng/µL.

FAQ ID - 3984

For the BioCalculator software, the default data folder is:

C:\Program Files\QIAGEN\QIAxcel BioCalculator\Data.

For the ScreenGel software, the default data folders are:

C:\Documents and Settings\All Users\Application Data\QIAGEN\QIAxcel\ScreenGel\Data\Experiment (for a computer with Windows XP operation system) or C:\ProgramData\QIAGEN\QIAxcel\ScreenGel\Data\Experiment\ (for a computer with Windows 7 operating system).

Alternatively, you can more conveniently access the ScreenGel experiment data folder using the menu options File\Open data directory\Experiment within the ScreenGel software.

FAQ -9008

If the gel cartridge tips for the QIAxcel System and QIAxcel Advanced are in contact with air, the tips will dry out. There are 3 ways of preventing the tips from drying.

Store the cartridge in the instrument with the instrument in the “Park” position. The washing solution (8 ml) covered with mineral oil must be in the “Park” position of the Solution Tray.
Store the cartridge in the Cartridge Stand with enough mineral oil in the well of the Cartridge Stand to cover the capillary tips. The Cartridge Stand should only be used for short term storage, i.e. when changing cartridges during one working day.
Place the cartridge back into its packaging with the capillary tips gently inserted into the soft gel in the box.

FAQ ID -1823

The reference size marker table created by ScreenGel version 1.1 or earlier is incompatible with ScreenGel version 1.2. Re-analyze the size marker run in ScreenGel version 1.2 and create a new reference size marker table for future use.

FAQ -9009

When using the BioCalculator software, ensure the color of the software key matches the type of cartridge used. For example, the QIAxcel Fast Analysis cartridge requires the red software key, while the QIAxcel High Resolution cartridge requires the blue software key.

In addition, check that the communication between the computer and the instrument is established. Further, check that the smart key is inserted securely.

If the issue persists, contact QIAGEN Technical Services and provide the cartridge ID and lot number, which is printed on the packaging of QIAxcel cartridges.

FAQ -9004

When using the BioCalculator software, select Settings under File in the Instrument control panel window. In the new windows, click Check Filter in the new window and follow the on-screen prompts.

When using the ScreenGel software, log in to the ScreenGel software with an account with basic or advanced privilege. Select System Check under the Service environment. Select Filter Check and click Start.

Please send the filter test result to QIAGEN Technical Services for further assistance.

FAQ -9006

Incorrect storage can lead to QX alignment marker degradation. The QX alignment markers can be used for 50 runs or 3 days, whichever comes first. Opened QX alignment marker should be store at –20°C between uses.

FAQ -9003

Please delete the 6 obsolete method files as outlines in the Important Note QIAxcel ScreenGel Software

FAQ -9010

When using the BioCalculator software, select Detector test under File in the Instrument control panel window. Perform the Detector test with and without a cartridge latched inside the QIAxcel. Feedback is not shown on the screen when you select the Detect test. Instead, the file named detector.asc is saved at C:\Program Files\QIAGEN\QIAxcel BioCalculator. Change the file name before performing another detector test.

Note: Older versions of BioCalculator software save the detector.asc at C:\Program Files\QIAxcel BioCalculator.

When using the ScreenGel software, log in to the ScreenGel with an account with basic or advanced privilege. Select System Check under the Service environment. Select Detector Test and click Start. The file is saved at C:\Documents and Settings\All Users\Application Data\QIAGEN\QIAxcel\ScreenGel\Data\Troubleshooting\Troubleshooting_xxxxxxxx (current date in the format of YYYYMMDD) for a computer with Windows XP operating system or C:\ProgramData\QIAGEN\QIAxcel\ScreenGel\Data\Troubleshooting for a computer with Windows 7 operating system. Alternatively, you can perform a screen capture for the Detector test result.

Please send the Detector test results to QIAGEN Technical Services for further assistance.

FAQ -9006