QIAxcel RNA QC Kit v2.0

使用QIAxcel系统进行自动化定性和定量RNA分析

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QIAxcel RNA High Sensitivity Kit (1200)

Cat. No. / ID: 929112

QIAxcel RNA High Sensitivity Gel Cartridge, Buffers, Mineral Oil, QX Intensity Calibration Marker, QX RNA Alignment Marker, QX RNA Size Marker 200–6000 nt, QX RNA Denaturation Buffer, 12-Tube Strips

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Cartridge type

High Sensitivity

RNA QC

Note: The QIAxcel High Sensitivity Kit only works with the QIAxcel Connect

QIAxcel RNA QC Kit v2.0 旨在用于分子生物学应用。这些产品不能用于疾病诊断、预防和治疗。

✓ 全天候自动处理在线订单

✓ 博学专业的产品和技术支持

✓ 快速可靠的（再）订购

特点

采用安全、操作简单的即用型预制胶
简化的工作流程，分析时间最短化
可检测到低至5 ng/μl的总RNA，结果可靠
比琼脂糖凝胶电泳更高的灵敏度
标准化的处理过程，包括数字化数据采集

产品详情

配合QIAxcel体系，QIAxcel RNA QC Kit v2.0每批可全自动定量和定性分析多达96个RNA样本。全自动上样和分析减少了对样本的手工操作，将RNA降解和污染的风险降到最低。该体系可检测低至5 ng/μl的稀释后总RNA（原始浓度10 ng/µl）和50 ng/µl的cRNA或单链cDNA。

绩效

快速、灵敏的分析RNA

在基因表达分析和miRNA研究中，RNA的完整性是获得可靠结果的一个关键因素，用于一些敏感的技术如定量PCR和微芯片分析。由于它们会强烈影响检测方法的预测能力，监测初始总RNA样本以及整个流程的产物的完整性是至关重要的。全自动QIAxcel仪能够快速、灵敏的分析总RNA和片段或完整的cRNA的完整性以及对它们进行定量。QIAxcel仪可方便的分析样本的降解水平以及完整性（参见" Analysis of degraded RNA"）。全自动上样和分析减少了对样本的手工操作，将RNA降解和污染的风险降到最低（参见" Analysis of plant RNA"）。

在QIAxcel仪上进行RNA分析高度可靠，并获得与Agilent Bioanalyzer相当的结果（见表）。

高度可靠的结果
75°C条件下的时间（分钟）	QIAxcel 1 28S/18S比值	QIAxcel 2 28S/18S比值	Agilent Bioanalyzer 28S/18S比值
0	1.59	1.53	1.7
2	1.42	1.55	1.6
4	1.31	1.45	1.6
7	1.35	1.36	1.5
13	1.16	1.18	1.2
20	1.09	1.07	1.3
30	0.68	0.72	1.1
40	0.58	0.53	0.8
52	0.64	0.61	0.5
70	0.34	0.38	0.3

查看图表

Analysis of degraded RNA.

Analysis of plant RNA.

原理

QIAxcel体系采用毛细管凝胶电泳技术，能够依据核酸的大小进行快速分离（参见""）。不同于传统的琼脂糖凝胶电泳，分离是在含有即用型预制胶的毛细管中进行。样本自动上样到单个的毛细管中，然后加压。带负电的核酸通过毛细管移到正极。使用琼脂糖凝胶电泳时，小分子核酸比大分子核酸移动得快。待测分子在毛细管中迁移，它们会通过一个检测和衡量荧光信号的检测器。光电倍增管将荧光信号转为电子数据，然后传输到电脑上再使用QIAxcel ScreenGel软件进一步处理。处理后，可通过电泳图谱和胶图两种方式查看数据。

查看图表

Sample separation process.

程序

QIAxcel RNA QC Kit v2.0简化了分离和分析RNA的流程，且更加安全。只需简单的几个步骤即可：装载预制胶卡夹、预装缓冲液槽及装载有样本的96孔板或PCR试管或联管、选择分离方法。无需冗长的制胶及用户培训，简化了日常工作的整个流程。运行开始几分钟内，会在电脑屏幕上实时出现第一个结果。

应用

QIAxcel RNA QC Kit v2.0能够快速、灵敏的分析总RNA和cRNA（片段或完整的）的完整性以及对它们进行定量检测。

辅助数据和图表

Sample separation process.

Nucleic acid molecules are separated according to size in gel-filled capillaries.

Streamlined RNA analysis using the QIAxcel system.

资源

A protocol for evaluating quality of RNA samples using the QIAxcel Advanced Instrument and ScreenGel software version 1.3 or higher

Download Safety Data Sheets for QIAGEN product components.

For automated quantitative and qualitative RNA analysis using the QIAxcel and QIAxcel Advanced instruments

For automated quantitative and qualitative RNA analysis using the QIAxcel Connect instrument

For automated quantitative and qualitative RNA analysis using the QIAxcel and QIAxcel Advanced instruments

For use with QIAxcel Advanced instruments and QIAxcel ScreenGel Software version 1.6

FAQ

No, the RNA gel itself is not denaturing. It is a native polymer gel.

FAQ ID - 3366

When analysing RNA Integrity Score with the QIAxcel RNA High Sensitivity Kit - samples must be in the concentration range of 1 ng/µL - 50 ng/µL.

FAQ ID - 3984

When using the BioCalculator software, select Detector test under File in the Instrument control panel window. Perform the Detector test with and without a cartridge latched inside the QIAxcel. Feedback is not shown on the screen when you select the Detect test. Instead, the file named detector.asc is saved at C:\Program Files\QIAGEN\QIAxcel BioCalculator. Change the file name before performing another detector test.

Note: Older versions of BioCalculator software save the detector.asc at C:\Program Files\QIAxcel BioCalculator.

When using the ScreenGel software, log in to the ScreenGel with an account with basic or advanced privilege. Select System Check under the Service environment. Select Detector Test and click Start. The file is saved at C:\Documents and Settings\All Users\Application Data\QIAGEN\QIAxcel\ScreenGel\Data\Troubleshooting\Troubleshooting_xxxxxxxx (current date in the format of YYYYMMDD) for a computer with Windows XP operating system or C:\ProgramData\QIAGEN\QIAxcel\ScreenGel\Data\Troubleshooting for a computer with Windows 7 operating system. Alternatively, you can perform a screen capture for the Detector test result.

Please send the Detector test results to QIAGEN Technical Services for further assistance.

FAQ -9006