QIAxcel Advanced System

便利的DNA片段和RNA分析

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QIAxcel Advanced Instrument

Cat. No. / ID: 9001941

Capillary electrophoresis device: delivered with computer, QIAxcel ScreenGel Software, and 1-year warranty on parts and labor

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InstrumentSoftware

QIAxcel Advanced

QIAxcel ScreenGel Software

The QIAxcel Advanced Instrument has been discontinued. QIAGEN will maintain a stock and inventory of repair and spare parts for the QIAxcel Advanced Instrument, including providing associated repair services until December 31, 2029.

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The QIAxcel Advanced instrument is intended to be used only in combination with QIAxcel Kits for the applications described in the respective QIAxcel Kit handbooks.

✓ 24/7 automatic processing of online orders

✓ Knowledgeable and professional Product & Technical Support

✓ Fast and reliable (re)-ordering

Features

快速分析多达96个样本，无需人工介入
采用安全、操作简单的即用型预制胶
可检测到浓度低至0.1 ng/µl的核酸，结果可靠
标准化、准确的分析，分辨率可达3–5 bp
界面友好的分析软件，支持21 CFR part 11规章

Product Details

新颖的QIAxcel Advanced体系取代了传统的、费力的DNA和RNA胶分析，流线式工作流程，节省时间。QIAxcel Advanced体系是灵敏的、高分辨率的毛细管电泳系统，每次能全自动分析多达96个样本。可在3分钟内完成12个样本的DNA片段分析。即用型预制胶分析96个样本，使用最少的转运时间，减少手动操作的错误，无需繁琐的制胶过程。界面友好的QIAxcel ScreenGel软件简化了分析和数据采集。

Performance

简化的工作流程

QIAxcel Advanced体系无需平板胶分析过程，加快了DNA和RNA分析流程，并且简化了整个样本纯化和分析过程（参见"Streamlined workflow"）。配合经验证的QIAGEN终点PCR试剂盒，QIAxcel Advanced体系提供一体化的、预先测试的方案，用于可靠地分析PCR片段，重复性好并有效的节省时间和经费。

一个仪器，多种应用

QIAxcel Advanced体系是多重电泳系统，适合多种应用。预设的方法配合相应的预制胶，可分离和分析多种核酸，包括单个和多重PCR片段、限制性内切酶消化的DNA、总RNA和cRNA。用于基因分型时，与传统的琼脂糖凝胶电泳相比，QIAxcel Advanced体系能够更加准确和灵敏的检测（参见"Accurate, high-throughput genotyping of bacteria"）。QIAxcel Advanced体系的高通量能力使其非常适合使用96孔纯化技术的实验室，需要一个快速的解决方案用于进行质量控制（参见"Analysis of purified RNA"）。不同于其他只能同时处理12个样本的仪器，QIAxcel Advanced体系能够同时处理96个样本，而无需手工介入。

Principle

QIAxcel Advanced系统包括一系列激光二极管和内置QIAxcel预制胶卡夹的微光采集器。片段在胶中移动的过程中毛细管旁路激发并检测信号，信号通过光电倍增管传至可进行数据分析的QIAxcel ScreenGel软件（参见"Sample separation process"）。

QIAxcel Advanced体系可进行高灵敏度的检测，即使分析低浓度核酸也可获得可靠的结果。小于0.5 kb的片段分辨率为3–5 bp，相比平板胶法QIAxcel Advanced体系确保更高的精确度，同时提供更可靠的数据分析。每次分析样本消耗量少于0.1 μl，为下游应用节约了珍惜样本。全自动上样和自控组件将接触如溴化乙锭等危险化学品的风险率降至最低（参见"Ready-to-run gel cartridges"）。

一系列QIAxcel Kits适合多种下游应用，满足不同的分辨率和速度需求（见表）。

QIAxcel Kits
QIAxcel Kit	被分析物	片段范围	100 bp – 500 bp*	500 bp – 1 kb*	1 kb – 5 kb*	5 kb – 10 kb*	运行时间/12个样本†
QIAxcel DNA High Resolution Kit	DNA	15 bp – 10 kb	3–5 bp	50 bp	200–500 bp	1–1.5 kb	7–20 min
QIAxcel DNA Screening Kit	DNA	15 bp – 5 kb	20–50 bp	50–100 bp	500 bp	–	5 min
QIAxcel DNA Fast Analysis Kit	DNA	15 bp – 3 kb	50–100 bp	100–250 bp	250 bp – 1 kb‡	–	3–5 min
QIAxcel RNA QC Kit v2.0	RNA	15 b – 6 kb	–	–	–	–	10 min

Procedure

QIAxcel Advanced体系只需简单的几步操作：预制胶卡夹、预装的缓冲液槽及装载有样本的96孔板或PCR管或联管、选择分离方法。无需冗长的制胶及用户培训，简化了日常工作的整个流程。运行开始几分钟内，会在电脑屏幕上实时出现第一个结果（参见"Reduced run times"）。

Applications

QIAxcel Advanced体系提供多种优化的应用，非常适合学术研究以及药学、生物技术和生物化学实验室。预设的方法配合相应的预制胶，可分离和分析多种核酸，包括单个和多重PCR片段、限制性内切酶消化的DNA、合成的寡聚核苷酸、总RNA和cRNA。

Software

QIAxcel ScreenGel软件，更加方便

QIAxcel ScreenGel软件专为QIAxcel Advanced体系设计，是功能强大、界面友好的软件，用于数据采集和分析。可通过电泳图谱和胶图两种方式查看数据，交互式工具简化了分析，便于快速解释数据。可单独或同时查看结果，用于对比样本或数据。对于多个数据进行一体化的分析方式简化了评估过程，独特的数学计算法则可以表格形式显示各种峰的特性，包括峰的数目、高度、宽度和每个峰的面积。可简单生成数据对比报表，保存并输出满足需要的文档（参见"Simplified and standardized data collection and analysis"）。

易于安装和启动

Process Wizard简化了实验启动过程（参见"Easy-to-use software features"）。这一方便的特性可引导安装、获得较好的运行参数和选择DNA大小标记。也包含试剂的批号信息。只需单击几下鼠标即可选择样本，且运行检查也很容易。

支持21 CFR Part 11 compliance

QIAxcel ScreenGel软件支持21 CFR Part 11规章的技术要求。安全特性包括：

输入密码登陆，防止未授权访问和数据处理
审查跟踪配置文件和系统事件
自动保存和存档写保护的原始数据
安全的用户管理

不同的用户配置文件均可（Routine、Basic、Advanced和Admin），且密码保护（需用户登陆）提高安全性。简单的检索界面，用户无需培训，对于没有经验的使用者该软件特别具备吸引力。

Services

Are you thinking of upgrading to the latest QIAxcel generation, the QIAxcel Connect?

Then QIAGEN’s trade-in/trade-up program is just right for you!

QIAGEN makes it easy to keep up to speed with the latest automation and sample processing technologies. Simply trade up your old QIAxcel Advanced to the QIAxcel Connect, or trade in a competitor instrument to the QIAxcel Connect.

Learn more about trade-in/trade-up opportunities.

Resources

For calibration of signal intensity

For optimal DNA size determination

For automated quantitative and qualitative RNA analysis using the QIAxcel and QIAxcel Advanced instruments

For optimal RNA fragment size determination

For automated quantitative and qualitative RNA analysis using the QIAxcel and QIAxcel Advanced instruments

For automated analysis of DNA fragments using the QIAxcel and QIAxcel Advanced instruments

For optimal use of the QIAxcel Gel Cartridges

This release includes the first version of QIAxcel ScreenGel NGS Profiles. These profiles are intended to be used to run and analyze reactions of GeneRead QIAact AIT and BRCA 1/2 panels using the QIAxcel instrument and QIAxcel ScreenGel 1.5 Software. These profiles are composed of an Process Profile, Analysis Profile, Distribution Profile, and Report/Export Profile.

This software may only be downloaded by registered users with a valid QIAxcel ScreenGel Software license. If you do not have a valid software license, contact your QIAGEN sales representative.

These profiles are intended to be used to run and analyze samples together with the QX DNA Size Marker Large-Fragment Kit (SAP 929710) on the QIAxcel instrument and QIAxcel ScreenGel 1.6 Software.

A protocol for evaluation of samples for next-generation sequencing on the QIAGEN GeneReader platform using the QIAxcel Advanced Instrument and ScreenGel software version 1.5 of higher

A protocol for evaluating quality of RNA samples using the QIAxcel Advanced Instrument and ScreenGel software version 1.3 or higher

Addressing critical factors and new solutions

For analysis and typing of PCR products from different HLA loci using the QIAxcel Advanced and the Helmberg-SCORE software

Method file for use with QIAxcel ScreenGel Software

Alignment marker file QX15/600 bp - English (XAM)

Method file for use with BioCalculator Software

Method file for use with QIAxcel ScreenGel Software

Process Profile and Method file for use with QIAxcel ScreenGel Software

Method file for use with BioCalculator Software

The QIAxcel System was successfully used to detect DNA derived from genetically modified organisms (GMOs) at a level suitable for GMO testing according to EU standards.

For effortless DNA and RNA analysis

For use with QIAxcel Advanced instruments and QIAxcel ScreenGel Software version 1.6

For easy upgrade of QIAxcel ScreenGel Software Version 1.6.

For effortless DNA and RNA analysis

The QIAxcel System was successfully used to detect DNA derived from genetically modified organisms (GMOs) at a level suitable for GMO testing according to EU standards.

For analysis and typing of PCR products from different HLA loci using the QIAxcel Advanced and the Helmberg-SCORE software

Addressing critical factors and new solutions

A protocol for evaluation of samples for next-generation sequencing on the QIAGEN GeneReader platform using the QIAxcel Advanced Instrument and ScreenGel software version 1.5 of higher

A protocol for evaluating quality of RNA samples using the QIAxcel Advanced Instrument and ScreenGel software version 1.3 or higher

Alignment marker file QX15/600 bp - English (XAM)

Method file for use with QIAxcel ScreenGel Software

Method file for use with BioCalculator Software

Method file for use with QIAxcel ScreenGel Software

Method file for use with BioCalculator Software

Process Profile and Method file for use with QIAxcel ScreenGel Software

Method file for use with QIAxcel ScreenGel Software

For automated quantitative and qualitative RNA analysis using the QIAxcel and QIAxcel Advanced instruments

For optimal DNA size determination

For optimal RNA fragment size determination

For optimal use of the QIAxcel Gel Cartridges

For automated analysis of DNA fragments using the QIAxcel and QIAxcel Advanced instruments

For automated quantitative and qualitative RNA analysis using the QIAxcel and QIAxcel Advanced instruments

For calibration of signal intensity

For use with QIAxcel Advanced instruments and QIAxcel ScreenGel Software version 1.6

These profiles are intended to be used to run and analyze samples together with the QX DNA Size Marker Large-Fragment Kit (SAP 929710) on the QIAxcel instrument and QIAxcel ScreenGel 1.6 Software.

This software may only be downloaded by registered users with a valid QIAxcel ScreenGel Software license. If you do not have a valid software license, contact your QIAGEN sales representative.

For easy upgrade of QIAxcel ScreenGel Software Version 1.6.

FAQ

The QIAxcel System or QIAxcel Advanced works on the basis of fluorescence detection technology. Each channel has a separate excitation light source, with its own optical detection. So each gel cartridge channel is different in the fluorescence emission signal collection mode with its own background and/or baseline noise. When you use a new cartridge, calibration (intensity normalization) is necessary to equalize the variations.

FAQ ID -9019

First, check that the glass capillaries at the bottom of the cartridge are intact. You should see approximately 1 mm of glass capillary. Second, check that each tube has at least 10 µl sample volume.

Check the capillary is unblocked by performing the gel-droplet test as outlined in Appendix D in the QIAxcel DNA handbook.

Perform a detector test.

If the issue persists, please send data to QIAGEN Technical Service. The instructions for QIAxcel data folder can be found in FAQ ID-9008.

FAQ -9015

When the QIAxcel or QIAxcel Advanced is first installed and the Transport Locking assembly is removed, there is a possibility that the Transport lower stop is hitting the bottom. When the Transport Locking assembly is first removed, it is recommended to slowly pull up on the Transport assembly so that the lower stop is not hitting the bottom (be sure that the power is turned off before the Transport Lock is removed).

FAQ ID -9017

Yes, if a different method is used with the QIAxcel System or QIAxcel Advanced, a new reference marker table needs to be created because of different relative migration time.

Please follow the instructions described in the manual and run the DNA marker and the Calibration marker with the new method.

FAQ ID -1857

When running RNA samples on the QIAxcel System or QIAxcel Advanced, the standard RNA denaturation process has to be followed prior to running the sample.

Add an equal volume of QX RNA Denaturation buffer to your RNA sample and/or QX RNA size Marker.
Heat the solution at 70°C for 2 minutes on a heating block or in a PCR machine, then place on ice for 1 minute.
Bring the total sample volume to 10 μl using QX RNA Dilution Buffer and mix the solution by gently pipetting up and down a few times.
Analyze the samples immediately.

FAQ ID -1842

Make sure the Gel Cartridge has been calibrated with the computer currently connected. If not, please re-calibrate the Gel Cartridge.

Make sure the cartridge has equilibrated to room temperature (20°–25°C [68°– 77°F]) and stood upright in the cartridge stand for 20 min prior to use. Empty the buffer tray.

Wash the buffer tray in warm water and rinse thoroughly with deionized or reverse-osmosis water. Refill the buffer tray. Perform a control run with the alignment marker and bland samples (10 μl QX DNA Dilution Buffer). The marker bands should now appear within the chosen separation time.

FAQ ID -1833

Refer to Appendix D of the QIAxcel DNA Handbook for hot water soaking and gel droplet testing. The handbook can be accessed by going to the Resources tab in the link below:

http://www.qiagen.com/products/catalog/automated-solutions/detection-and-analysis/qiaxcel-dna-kits#

FAQ ID -1838

The intensity calibration procedure on the QIAxcel System and QIAxcel Advanced is performed to normalize the signal intensities across all 12 channels of the gel cartridge. This corrects for natural intensity reading variations between each capillary in the cartridge. A correction factor is determined and applied for every subsequent run performed with the cartridge.

FAQ ID -1824

Unplug the QIAxcel or QIAxcel Advanced instrument and remove the fuse holder to verify that the fuses are good. If not, please replace the fuses (Cat No. 9241178 Fuse, 4A, 250VAC, QX). If this does not correct the problem, contact QIAGEN Technical Services.

FAQ ID -1851

If you hear a 'hissing" sound when the QIAxcel System or QIAxcel Advanced is purging, remove the Gel Cartridge and verify that the Cartridge Purge Port label is removed.

FAQ ID -9018

If lower and upper alignment markers used with the QIAxcel System or QIAxcel Advanced are not aligned correctly, open the channel of the sample that was incorrectly aligned. Check whether any unexpected extra peaks have been detected or if the alignment marker peaks do have any “shoulders” that are detected as separate peaks. Delete any extra peaks if necessary and re-analyze your data.

FAQ ID -1834

The Gel Cartridge does not need to be recalibrated when using a new alignment marker and/or DNA size marker with the QIAxcel System or QIAxcel Advanced. Calibration is required only once, i.e. when the first cartridge is run or when the cartridge is used on a different QIAxcel system than the one it has been calibrated on, or when using a different PC than the one used to calibrate initially.

FAQ ID -1858

The revolutionary QIAxcel Advanced System replaces traditional, labor-intensive gel analysis of DNA and RNA.

Rapid analysis of up to 96 samples without manual intervention
Safety and convenience with ready-to-use gel cartridges
Robust results for nucleic acid concentrations as low as 0.1 ng/ul
Standardized and accurate analysis with a resolution down to 3-5 bp
User-friendly analysis software that supports 21 CFR Part 11 compliance

See: http://www.qiagen.com/Knowledge-and-Support/Videos-and-Virtual-Demos/Assay-Demos/QIAxcelAdvancedDemo/ for a virtual demonstration of the special software, features, and applications of the QIAxcel Advanced System.

FAQ ID -2650

The working alignment marker is an indication that the cartridge channel is working well. DNA samples that do not appear in all channels of the QIAxcel System or QIAxcel Advanced may be caused by:

Low sample volume (< 10 ul). The minimum required sample volume is 10 ul.
Trapped air bubble in the well. The air bubble must be removed.
High salt concentration in the sample. Dilute the sample with the QX DNA or RNA dilution buffer and rerun the sample.

FAQ ID -1844

Check that the lower and upper alignment markers are detected correctly, and that the threshold is set properly.

If the issue persists, please send data to QIAGEN Technical Service. The instructions for QIAxcel data folder can be found in FAQ ID-9008.

FAQ ID -9015

Verify that the Cartridge Purge Port label is removed. If it has been removed, verify that the alignment marker and samples are in the appropriate locations in the Buffer Tray and the 96-well Sample Tray used with the QIAxcel System or QIAxcel Advanced?.

FAQ ID -1847

When using this specific marker combination with the QIAxcel System or QIAxcel Advanced, i.e. for STR analysis, the 1.8 kb fragment lies outside of the range of detectable fragments and does not show up on the electropherogram and/or the gel view image.

FAQ ID -1835

N2 cylinders on the QIAxcel Advanced should be changed when one or both low pressure warnings are shown in the "Status Information" panel.

The left panel of the screen in the "Process" environment is called the "Status Information" panel.

"Pressure 1" status: OK/Low. OK indicates adequate pressure for the sample run. LOW indicates that the N2 pressure is sufficient for the current sample run; however, the N2 cylinder should be replaced once the run has finished.

"Pressure 2" status: OK/Low. OK indicates adequate pressure for the sample run. LOW indicates that the N2 pressure is insufficient for the current sample run and the analysis will not be performed. The N2 cylinder should be replaced.

FAQ ID -3001

The solutions used with the QIAxcel System or QIAxcel Advanced should not have to be changed for the life of the Gel Cartridge (based on the number of runs). However, if any contamination is suspected, the Buffer Tray should be cleaned and the buffers should be changed immediately.

FAQ ID -1840

If the gel cartridge tips for the QIAxcel System and QIAxcel Advanced are in contact with air, the tips will dry out. There are 3 ways of preventing the tips from drying.

Store the cartridge in the instrument with the instrument in the “Park” position. The washing solution (8 ml) covered with mineral oil must be in the “Park” position of the Solution Tray.
Store the cartridge in the Cartridge Stand with enough mineral oil in the well of the Cartridge Stand to cover the capillary tips. The Cartridge Stand should only be used for short term storage, i.e. when changing cartridges during one working day.
Place the cartridge back into its packaging with the capillary tips gently inserted into the soft gel in the box.

FAQ ID -1823

The minimum sample volume required for the QIAxcel System and QIAxcel Advanced is 10 ul to guarantee sample injection in each channel.

FAQ ID -1852

If one of the capillaries of the gel cartridge for the QIAxcel System or QIAxcel Advanced is clogged, the corresponding lane in the gel-view image is typically black.

FAQ ID -1836

When running a QIAxcel Advanced instrument with the BioCalculator software, ensure to use version v3.2.07 or higher. You can download the latest version of the software from:

BioCalculator: http://www.qiagen.com/resources/Download.aspx?id=b68d8727-f0d7-44e5-acc5-2bb6ed50b63d&lang=en&ver=1

ScreenGel: http://www.qiagen.com/Products/Catalog/Automated-Solutions/Detection-and-Analysis/QIAxcel-ScreenGel-Software#resources

FAQ ID -9016

When running RNA samples on the QIAxcel System or QIAxcel Advanced, the standard RNA denaturation process has to be followed prior to running the sample.

Add an equal volume of QX RNA Denaturation buffer to your RNA sample and/or QX RNA size Marker.
Heat the solution at 70°C for 2 minutes on a heating block or in a PCR machine, then place on ice for 1 minute.
Bring the total sample volume to 10 μl using QX RNA Dilution Buffer and mix the solution by gently pipetting up and down a few times.
Analyze the samples immediately.

FAQ ID -9020

Yes, the QIAxcel System or QIAxcel Advanced can be connected to a local network.

FAQ ID -1862

The QIAxcel Advanced has been discontinued as of October 2022. This follows the launch of the QIAxcel Connect instrument, the next generation of the QIAxcel technology. QIAGEN will maintain a stock and inventory of repair and spare parts for the QIAxcel Advanced including providing associated repair services until December 31, 2029. Please contact your customer care or sales representative if you have any questions about the discontinuation of QIAxcel Advanced.

FAQ ID - 3983

The BioCalculator (version 3.2.05) and ScreenGel software have a feature to close the pressure valve after the software is idled for more than 30 minutes. To re-open the pressure valve, execute a command with the software, such as move the buffer tray, unlatch and latch the cartridge; alternatively, you can also exit the software and re-launch it. Also see FAQ ID 3001.

FAQ -9011

The sample DNA concentration might be too low. You can increase sample injection time.

A very strong sample in the same run can cause the threshold to be set too high. As a result, the signal from weaker samples is below this threshold. Try diluting the sample with high concentration with the QX dilution buffer.

High salt concentration in the samples, such as restriction enzyme digested DNA, can interfere with sample injection. Try reducing the salt concentration by diluting such samples with the QX dilution buffer.

If the issue persists, please send data files to QIAGEN Technical Services. See <a href="/knowledge-and-support/faq/?ID=71818D37-21EF-40B0-BA30-6C8CC8CA82B9"><b>Where are the QIAxcel data stored</a></b>.

FAQ -9013

High or very low salt concentration could lead to the described appearance when running samples on the QIAxcel System and QIAxcel Advanced. Make sure QX DNA Dilution buffer has been used to dilute your samples. Dilute your samples 1:2 in QX DNA Dilution buffer and re-analyze.

FAQ ID -1837